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EC number: 205-182-7 | CAS number: 135-19-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: A well documented study according to OECD and GLP guidelines. In the OECD SIDS on 2-Naphthol (2002) a similar reliability was assigned.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 005
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 474 (Mammalian Erythrocyte Micronucleus Test)
- GLP compliance:
- yes
- Type of assay:
- micronucleus assay
Test material
- Reference substance name:
- 2-naphthol
- EC Number:
- 205-182-7
- EC Name:
- 2-naphthol
- Cas Number:
- 135-19-3
- Molecular formula:
- C10H8O
- IUPAC Name:
- 2-naphthol
- Details on test material:
- Purity:99.5%
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- other: BDF1 (C57BL/6 x DBA/2)
- Sex:
- male
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- 2-naphthol was given suspended in 0.5% methylcellulose solution.
Dosage volume was 0.1 mL/kg bw in the dose-finding and the main tests. - Duration of treatment / exposure:
- Exposure period: 2days
- Frequency of treatment:
- Once daily for consecutive two days with 24-hour intervals.
- Post exposure period:
- Mice were observed for the mortality for 24 hrs after the second administration.
Doses / concentrations
- Remarks:
- Doses / Concentrations:
0 (control), 62.5, 125 or 250 mg/kg bw
Basis:
other: The doses in the main test were determined based on the results of the dose-finding test where the following doses were tested 62.5, 125, 250, 500 or 1000 mg/kg bw.
- No. of animals per sex per dose:
- Dose finding test: male and female mice (three/sex/group) were given 2-naphthol suspended in 0.5% methylcellulose solution at 62.5, 125, 250, 500 or 1000 mg/kg bw
Main test: Six male mice at 0 (control), 62.5 and 125 mg/kg bw, and eight male mice at 250 mg/kg bw.
Five specimens per group were examined. - Control animals:
- yes, concurrent vehicle
- Positive control(s):
- Control animals were given 0.5% methylcellulose solution only by gavage.
Dosage volume was 0.1 mL/kg bw in the dose-finding and the main tests.
Positive control animals were once injected intraperitoneally with mitomycin C at 0.5 mg/kg bw.
Examinations
- Tissues and cell types examined:
- The animals were killed 24 hours after the second administration, and slides were prepared for bone marrow cells obtained from the femurs. Five specimens per group were examined.
Results and discussion
Test results
- Sex:
- male
- Genotoxicity:
- negative
- Toxicity:
- not specified
- Vehicle controls validity:
- valid
- Negative controls validity:
- not specified
- Positive controls validity:
- valid
- Additional information on results:
- RESULTS OF RANGE-FINDING STUDY
In the dose-finding test (62.5, 125, 250, 500 or 1000 m/kg bw, p.o.), mortalities were in 3/3 (dead mice/mice treated) at 500 and 1000 mg/kg bw in males, and in 1/3 at 250 mg/kg bw and 3/3 at 500 and 1000 mg/kg bw in females. There was no difference between sexes in mortality in the dosefinding test.
RESULTS OF DEFINITIVE STUDY
In the main test (micronucleus assay), this chemical showed no induction of micronuclei in born marrow polychromatic erythrocytes. The results of micronucleus assay were negative overall.
Dose (mg/kg bw) 0 62.5 125 250
No. of animals examined 5 5 5 5
Frequency of MNPCE (%) Mean 0.18 0.27 0.22 0.29
SD 0.08 0.10 0.04 0.09
Range of MNPCE/2000PCE 2-6 3-8 3-5 3-8
Ration of PCE(%) Mean 57.6 54.6 54.8 48.7
SD 5.8 2.1 9.8 7.9
Note: MNPCE: Micronucleated polychromatic erythrocyte
PCE : Polychromatic erythrocyte
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information): negative
An in vivo micronucleus test of 2-naphthol was performed with bone marrow cells of male BDF1 mice. 2-Naphthol did not induce micronuclei in the bone marrow cells at up to 250 mg/kg.
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