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EC number: 220-237-5 | CAS number: 2680-03-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Specific investigations: other studies
Administrative data
- Endpoint:
- biochemical or cellular interactions
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Acceptable publication which meets basic scientific principles.
Data source
Reference
- Reference Type:
- publication
- Title:
- Effect of acrylamide and related compounds on glycolytic enzymes in rat sciatic nerve in vivo
- Author:
- Sakamoto J, Hashimoto K
- Year:
- 1 985
- Bibliographic source:
- Arch Toxicol (1985) 57: 282-284
Materials and methods
Test guideline
- Qualifier:
- no guideline required
- GLP compliance:
- not specified
- Type of method:
- in vivo
Test material
- Reference substance name:
- N,N-dimethylacrylamide
- EC Number:
- 220-237-5
- EC Name:
- N,N-dimethylacrylamide
- Cas Number:
- 2680-03-7
- Molecular formula:
- C5H9NO
- IUPAC Name:
- N,N-dimethylacrylamide
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Weight at study initiation: 192±8 g
- Housing: three to four per plastic cage containing wooden flakes
- Diet (e.g. ad libitum): laboratory chow
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 23±2
Administration / exposure
- Route of administration:
- oral: drinking water
- Vehicle:
- unchanged (no vehicle)
- Analytical verification of doses or concentrations:
- not specified
- Duration of treatment / exposure:
- 15, 30 and 45 days
- Frequency of treatment:
- daily
Doses / concentrations
- Remarks:
- Doses / Concentrations:
15 mM
Basis:
nominal in water
- No. of animals per sex per dose:
- 5
- Control animals:
- yes, concurrent no treatment
- Details on study design:
- Preparation of sciatic nerve for enzyme assay. Rats were killed by decapitation after the three different dosing periods, and the sciatic nerves of both lower extremities were quickly removed from the sciatic notch to the ankle region of the tibial nerve with a part of the common peroneal nerve, weighed, frozen in liquid N2, and stored at -80ºC until use. The frozen nerves were crushed with a stainless steel tissue crusher pre-chilled in liquid N2, suspended in 10 vol ice-cold 0.05 M tris-phosphate buffer, pH 7.4, containing 1 mM EDTA and 1 mM dithiothreitol, and gently homogenized using a Potter-Elvehjem homogenizer with teflon pestle. The homogenates were centrifuged at 100,000 g for 60 min at 4ºC and the resultant supernatants were used for enzyme assays.
Enzyme assays. Activity of both GAPDH and total enolase in sciatic nerve samples was determined as described in the report by Sakamoto and Hashimoto (1985). The protein content of the assay samples was determined by the method Lowry et al. (1951).
Statistical method. Comparisons of mean values in the test groups versus the controls were performed using the Student's t-test.
Results and discussion
- Details on results:
- After 15 days of treatment, N,N-dimethylacrylamide, a non-neurotoxic analogue of acrylamide, produced moderate body weight loss without causing any change in the activity of either enzyme (GAPDH and enolase). After 30 days of treatment N,N-dimethylacrylamide produced body weight loss and reduction in GAPDH activity. After 45 days of treatment, N,N-dimethylacrylamide also produced body weight loss and inhibition of GAPDH.
N,N-dimethylacrylamide produced inhibition of GAPDH as well as body weight loss after 30 days without causing any signs of neuropathy. This compound did not inhibit enolase even after 45 days' treatment, although it has a strong inhibitory effect on mouse brain total enolase (Sakamoto and Hashimoto 1985). From the present observations, it might be suggested that even non-neurotoxic acrylamide analogues have an inhibitory effect on GAPDH in vivo.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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