Registration Dossier
Registration Dossier
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EC number: 225-533-8 | CAS number: 4904-61-4
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1989-05-03 to 1989-05-31
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 997
- Report date:
- 1997
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)
- Version / remarks:
- Cited as Directive 84/449/EEC, C.6
- GLP compliance:
- no
Test material
- Reference substance name:
- 1,5,9-cyclododecatriene
- IUPAC Name:
- 1,5,9-cyclododecatriene
- Details on test material:
- - 1,5,9-cyclododecatriene of Hüls AG
- Sample ID 892/890303
- no data on purity
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- sewage, predominantly domestic (adaptation not specified)
- Details on inoculum:
- INOCULUM
- Sampling site: predominantly domestic WWTP Marl-Ost, sampled 03 May 1989
- Preparation of inoculum: filtration (aerobic), the first 200 ml of the filtrate were discarded
- Initial inoculum concentration: 0.5 ml/l - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 2 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST SYSTEM
- Culturing apparatus: 300 ml precision glass bottles
- Number of culture flasks per concentration: 2 per sampling time (0, 5, 15, and 28 days)
- Measuring equipment: oxygen electrode WTW
- Closed vessels used: yes
TEST CONDITIONS
- Test temperature: 20 degree C
SAMPLING
- after 0, 5, 15 and 28 days
ANALYTICAL PARAMETER: dissolved oxygen (WTW probe)
Reference substance
- Reference substance:
- benzoic acid, sodium salt
- Remarks:
- Purity > 99.5% (Fluka)
Results and discussion
% Degradation
- Parameter:
- % degradation (O2 consumption)
- Value:
- 0
- Sampling time:
- 28 d
- Details on results:
- Degradation products: not measured
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- The test substance was observed to be not ready biodegradable in the present test.
- Executive summary:
The test substance was observed to be not ready biodegradable in the "Closed Bottle Test" conducted by Hüls AG (1997) according to EU method C.4 -E. 0% degradation was observed with in a period of 28 days. The study was assessed as "reliable without restrictions".
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