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EC number: 474-870-9 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 09 July 2007 to 10 August 2007
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- Sampling schedule:
Control : at 0 and 48 hours
Test concentrations : at 0 and 48 hours - Vehicle:
- no
- Details on test solutions:
- 105.1 mg of the test item were added to 1 litre of dilution water.
Culture and dilution water
Reconstituted water (so-called 'M4 medium', originally described in Water Research 24 (9), September 1990: 1157-1167), prepared according to the recommendations of Bundesgesundheitsamt Berlin. This standard dilution water is used for both, the maintenance of the test animals and the preparation of stock and test solutions of the test item. The total hardness of the dilution water, measured at test start, was 15.4°dH (= 275 mg/L CaCO3). - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Name : Daphnia magna STRAUS, parthenogenetic females
Source : Strain of Bundesgesundheitsamt Berlin
Maintenance and Acclimatisation : A population of parthenogenetic females of synchronised age structure is maintained since more than 15 years in the test facility under constant temperature conditions (20 +/- 1 °C) at a 16 : 8 hour light-dark photoperiod (illumination: < 1000 lux). The culture water (so-called 'M4 medium') is partly renewed once a week. The Daphnia are exclusively fed with unicellular green algae (Desmodesmus subspicatus) 'ad libitum'. Mortalities of parent Daphnia during the culture period are recorded daily in a semi-quantitative way. The neonates are separated from their parent Daphnia by filtration prior to the acute test. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Post exposure observation period:
- No post exposure observation period
- Hardness:
- 275 mg CaCO3/L
- Test temperature:
- 20.9C
- pH:
- 7.7
- Dissolved oxygen:
- 8.6 mg/l
- Salinity:
- Not applicable.
- Nominal and measured concentrations:
- 0 and 100 mg/l
- Details on test conditions:
- Test vessels : 50 ml glass beakers holding 10 neonates in 20 ml of test medium
Experimental design : 1 test concentration plus 1 control
10 neonates per vessel, 2 replicates per concentration/control
no feeding during the exposure period
static system
Method of initiation : neonates were placed in prepared media
Photoperiod : 16 h light : 8 h dark
Temperature : mean +/- 1 °C (in the temperature range 18-22 °C)
Aeration : none
Test item concentration/s : 100 mg/l
Method of administration : direct weighing
Medium renewal : none
Duration of exposure : 48 hours
Criteria of effects : The criterion of adverse effects used in this study was the item-induced alteration of the normal mobility behaviour and the loss of locomotory actions of the neonates, observed at 24 and 48 hours. - Reference substance (positive control):
- no
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- No toxic effects against daphnia at a test concentration of 100 mg/l.
The results are expressed in terms of nominal concentrations. Recovery rates correspond to 104.7% of nominal values at 0 hours, and to 99.8% of nominal values at 48 hours, respectively. - Results with reference substance (positive control):
- Not applicable.
- Reported statistics and error estimates:
- The results are expressed in terms of nominal concentrations. Recovery rates correspond to 104.7% of nominal values at 0 hours, and to 99.8% of nominal values at 48 hours, respectively.
- Validity criteria fulfilled:
- yes
- Conclusions:
- Analysis of the immobilisation rates gives the following results:
Time EC 50
[h] [mg/l]
24 > 100
48 > 100
No toxic effects against daphnia at a test concentration of 100 mg/l.
The results are expressed in terms of nominal concentrations. Recovery rates correspond to 104.7% of nominal values at 0 hours, and to 99.8% of nominal values at 48 hours, respectively. - Executive summary:
A study was performed to assess the acute toxicity of Gelb-sulfato to Daphnia magna STRAUS under static conditions.
The study was conducted in accordance with the OECD Guideline for Testing of Chemicals No. 202 'Daphnia sp., Acute Immobilisation Test' which is in most parts equivalent to the EEC Methods for Determination of Ecotoxicity Annex to Directive 92/69/EEC Part C, Method 2 'Acute toxicity for Daphnia'. Study performed in accordance with the Principles of Good Laboratory Practice (GLP) and reported with a GLP certificate.
The Daphnia were exposed to a limit test concentration of nominally 100 mg/l of Gelb-sulfato dissolved in water.
Observations were made on the swimming ability and the immobilisation rate, respectively, after 24 and 48 hours of exposure. The following values were determined:
Time EC 50
[h] [mg/l]
24 > 100
48 > 100
No toxic effects against daphnia at a test concentration of 100 mg/l.
The results are expressed in terms of nominal concentrations. Recovery rates correspond to 104.7% of nominal values at 0 hours, and to 99.8% of nominal values at 48 hours, respectively.
Reference
RESULTS
Immobilisation forDaphnia magna (initial population: 20) |
24h |
48h |
Test item concentration [mg/l]: Control |
||
Absolute |
0 |
0 |
Cumulative |
0 |
0 |
Cumulative [%] |
0 |
0 |
Test item concentration [mg/l]: 100 |
||
Absolute |
0 |
0 |
Cumulative |
0 |
0 |
Cumulative [%] |
0 |
0 |
Abnormalities |
24h |
48h |
Test item concentration [mg/l]: Control |
||
Abnormal colouration |
0/20 |
0/20 |
Abnormal swimming behaviour |
0/20 |
0/20 |
Test item concentration[mg/l] 100 |
||
Abnormal colouration |
0/20 |
0/20 |
Abnormal swimming behaviour |
0/20 |
0/20 |
Daphnids with effects / daphnids mobile += all daphnids immobile
Description of key information
There was no toxic effects against daphnia at a test concentration of 100 mg/L. The EC0 and EC50 are above 100 mg/L.
Key value for chemical safety assessment
Additional information
A study was performed to assess the acute toxicity of Gelb-sulfato (= Reactive Yellow 176 Ester) to Daphnia magna STRAUS under static conditions. The study was conducted in accordance with the OECD Guideline for Testing of Chemicals No. 202 'Daphnia sp., Acute Immobilisation Test' which is in most parts equivalent to the EEC Methods for Determination of Ecotoxicity Annex to Directive 92/69/EEC Part C, Method 2 'Acute toxicity for Daphnia'. Study performed in accordance with the Principles of Good Laboratory Practice (GLP) and reported with a GLP certificate.
The Daphnia were exposed to a limit test concentration of nominally 100 mg/L of the test substance dissolved in water.
Observations were made on the swimming ability and the immobilisation rate, respectively, after 24 and 48 hours of exposure. The following values were determined:
Time EC50
[h] [mg/l]
24 > 100
48 > 100
The results are expressed in terms of nominal concentrations. Recovery rates correspond to 104.7% of nominal values at 0 hours, and to 99.8% of nominal values at 48 hours, respectively.
No toxic effects against daphnia at a test concentration of 100 mg/L The EC50 (48 h): > 100 mg/L test mat. based on: mobility
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