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EC number: 207-306-5 | CAS number: 460-19-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 870.1300 (Acute inhalation toxicity)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 403 (Acute Inhalation Toxicity)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Oxalonitrile
- EC Number:
- 207-306-5
- EC Name:
- Oxalonitrile
- Cas Number:
- 460-19-5
- Molecular formula:
- C2N2
- IUPAC Name:
- carbononitridic cyanide
- Test material form:
- gas
Constituent 1
- Specific details on test material used for the study:
- Purity: 97.5% w/w (0.4% w/w – hydrogen cyanide, inert gases-balance)
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- Source
Received from SAGE® Labs on August 24 and November 2, 2016.
Sex
5 Males and 5 Females. Females assigned to test were nulliparous and non-pregnant.
Age/weight at study initiation
Young adult (9–10 weeks)/males 288–376 g and females 190–252 g at experimental start
Administration / exposure
- Route of administration:
- inhalation: gas
- Type of inhalation exposure:
- nose only
- Vehicle:
- clean air
- Duration of exposure:
- 4 h
- Remarks on duration:
- postexposure period 14 days
- Concentrations:
- Exposure concentrations: 63.14 ppm, 128.02 ppm, 181.56 ppm
- No. of animals per sex per dose:
- 25 animals totally, three concentrations totally – For concentration 128.02 ppm – 5 animals (males only), for two other concentrations – 10 animals for each concentration
- Control animals:
- yes
Results and discussion
Effect levelsopen allclose all
- Key result
- Sex:
- male
- Dose descriptor:
- LC50
- Effect level:
- 136.45 ppm
- Key result
- Sex:
- female
- Dose descriptor:
- LC50
- Effect level:
- > 181.56 ppm
Applicant's summary and conclusion
- Interpretation of results:
- other: CLP Acute tox. 2 H220
- Conclusions:
- Acute inhalation toxicity GLP study, based on 4-hours nose-only exposure of albino rats, was performed. In this study 3 concentrations (63.14, 128.02, and 181,56 ppm) of ethanedinitrile were tested. Both males and females were tested only for two of the concentrations – 63.14 and 181.56 ppm, and males were tested also for the concentration 128.02 ppm. While at concentration of 63.14 ppm all of tested animals survived, all tested males and one female died at concentration 181.56 ppm within one day. One male died within one day at concentration 128.02 ppm. Based on these data, LC50 for ethanedinitrile was determined to 136.46 ppm for males and greater than 181.56 ppm for females.
- Executive summary:
Materials and Methods
Test material
Ethanedinitrile (NCCN)
Specification
Ethanedinitrile–gas
Purity
97.5% w/w (0.4% w/w–hydrogen cyanide, inert gases-balance)
Test Animals
Rat
Strain
Albino rat – Sprague-Dawley derived
Source
Received from SAGE® Labs on August 24 and November 2, 2016.
Sex
5 Males and 5 Females. Females assigned to test were nulliparous and non-pregnant.
Age/weight at study initiation
Young adult (9–10 weeks)/males 288–376 g and females 190–252 g at experimental start
Number of animals per group
25 animals totally, three concentrations totally – For concentration 128.02 ppm – 5 animals (males only), for two other concentrations – 10 animals for each concentration
Control animals
No
Administration/Exposure
Inhalation
Postexposure period
14 days
Concentration of test substance
Exposure concentrations: 63.14 ppm, 128.02 ppm, 181.56 ppm
Type of exposure
Nose-only
Duration of exposure
4 hours
Results and Discussion
First tested concentration – 63.14 ppm
All animals survived exposure to the test atmosphere. Following exposure,three animals exhibited nasal discharge and four animals exhibited irregular respiration. In addition, three males and one female exhibited ano-genital staining. However, the animals recovered by Day 3 and appeared active and healthy for the remainder of the 14-day observation period. No gross abnormalities were noted for the animals when necropsied at the conclusion of the 14-day observation period.
Second tested concentration – 128.02 ppm
One male died within one day of exposure to the test atmosphere. Prior to death, the animal was hypoactive and exhibited irregular respiration and prone posture. Following exposure, thesurviving animals were hypoactive, exhibited irregular respiration and/or prone posture. However, the animals recovered by Day 4 and appeared active and healthy and gained weight for the remainder of the 14-day observation period.Gross necropsy of the decedent revealed discoloration of the lungs and liver and distention of the intestines and stomach. No gross abnormalities were noted for the surviving animals when necropsied at the conclusion of the 14-day observation period
Third tested concentration – 181.56 ppm
Five males and one female died within one day of exposure to the test atmosphere.Prior to death, three animals were hypoactive and exhibited irregular respiration and prone posture. There were no toxic effects noted prior to death of the three remaining animals. Following exposure, the surviving animals were hypoactive exhibited irregular respiration and/or prone posture. However, the animals recovered by Day 3 and appeared active and healthy for the remainder of the 14-day observation period. Gross necropsy of the decedents revealed discoloration of the lungs and/or distention of the intestines and stomach. No gross abnormalities were noted for either of the surviving animals when necropsied at the conclusion of the 14‑day observation period.
LC50
Under the conditions of this study, the acute inhalation defined LC50 of ethanedinitrile calculatedby Probit Analysis was 136.46 ppm of the test substance per liter of air in male rats with 95% Confidence Limits of 170.84 (upper) and 109.01 ppm (lower). The data does not permit calculation of the LC50 for females by Probit Analysis. The LC50 for females is greater than 181.56 ppm.
Summary and conclusion
An acute inhalation toxicity test was conducted with rats to determine the potential for ethanedinitrile to produce toxicity from a single exposure via the inhalation (nose-only exposure) route. Experiments were performed according to guidelines mentioned above and in accordance with good laboratory practice (GLP). Under the conditions of this study, the acute inhalation defined LC50 of ethanedinitrile calculatedby Probit Analysis was 136.46 ppm of the test substance per liter of air in male rats with 95% Confidence Limits of 170.84 (upper) and 109.01 ppm (lower). The data does not permit calculation of the LC50 for females by Probit Analysis. The LC50 for females is greater than 181.56 ppm.
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