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EC number: 429-530-4 | CAS number: 96662-24-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 10 September 1998 to 11 September 1998
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study conducted to EU & OECD test guidance in compliance with GLP.
- Qualifier:
- according to guideline
- Guideline:
- other: Commission Directive 88/302/EEC; Official Journal of the EC L 133, Part C: Biodegradability: Test for inhibition of oxygen consumption (corresponds for the most part to the test method OECD 209)
- Deviations:
- no
- Principles of method if other than guideline:
- A defined quantity of activated sludge is mixed with synthetic medium and the respiratory rate is measured. This rate is compared to those measured in test preparations with various concentrations of the test substance. In order to check the sensitivity of the activated sludge used, 3.5-dichlorophenol is used as a reference substance.
Deviations from the SOPs with reasons : Incubation time of 3 hours with permanent aeration instead of 30 minutes incubation time. Reason: Recommendation of the German member state authority. - GLP compliance:
- yes
- Analytical monitoring:
- no
- Details on sampling:
- The test concentrations where not confirmed by analytical techniques or sampling.
- Vehicle:
- no
- Details on test solutions:
- The test substance has been added to about 130 ml deionized water and stirred overnight before testing (equilibration phase).
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Type: activated sludge (mixed population of different microorganisms)
Origin: aeration tank of a waste water plant treating predominantly domestic sewage (Wupper area water authority)
Pretreatment: none - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Post exposure observation period:
- No post exposure observation period
- Hardness:
- Not reported.
- Test temperature:
- 20.9 - 21.0
- pH:
- 7.5 - 8.0
- Dissolved oxygen:
- Start of study 5.8 - 6.3 mg O2/l
End of study 2.6 - 2.9 mg O2/l - Salinity:
- Not applicable.
- Nominal and measured concentrations:
- Test concentrations (mg/l): 100, 1000, 10000
Test substance concentration in physico-chemical oxygen consumption control (mg/l): 10000 - Details on test conditions:
- A defined quantity of activated sludge is mixed with synthetic medium and the respiratory rate is measured. This rate is compared to those measured in test preparations with various concentrations of the test substance. In order to check the sensitivity of the activated sludge used, 3.5-dichlorophenol is used as a reference substance.
Incubation time of 3 hours with permanent aeration instead of 30 minutes incubation time. Reason: Recommendation of the Environmental Federation Office.
The study is carried out with nominal weights. The test concentrations are not confirmed by chemical analysis.
Test concentrations (mg/l): 100, 1000, 10000
** Test substance concentration in physico-chemical oxygen consumption control (mg/l): 10000
Type of application: direct weighing
Test concentration of the activated sludge: 320 mg/l
Test temperature: 20±2°C
Incubation time : 3 hours with permanent aeration
** The physico chemical oxygen consumption control is carried out, since some substances can also consume oxygen by chemical reactivity. In order to be able to tell the difference between physico chemical oxygen consumption and biological oxygen consumption (respiration), at least the maximum concentration of the test substance is tested without activated sludge. - Reference substance (positive control):
- yes
- Remarks:
- 3.5-dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 10 000 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- Details under any other information
- Results with reference substance (positive control):
- Details under any other information
- Reported statistics and error estimates:
- No data
- Validity criteria fulfilled:
- yes
- Conclusions:
- Toxicity of T-9601 to activated sludge: EC 50 > 10000 mg/l
- Executive summary:
Study conducted to Commission Directive 88/302/EEC; Official Journal of the EC L 133, Part C: Biodegradability: Test for inhibition of oxygen consumption (corresponds for the most part to the test method OECD 209). Study in compliance with GLP.
A defined quantity of activated sludge is mixed with synthetic medium and the respiratory rate is measured. This rate is compared to those measured in test preparations with various concentrations of the test substance. In order to check the sensitivity of the activated sludge used, 3.5-dichlorophenol is used as a reference substance.
Toxicity of T-9601 to activated sludge : EC 50 > 10000 mg/l
Reference
Measured values of test substance / control / reference substance
|
Test concentration [mg/l] |
Initial concentration* [mg/250 ml] |
O2-start [mg O2/l] |
O2-end [mg O2/l] |
Time [min.] |
Temp. [°C] |
pH |
Test substance |
100 |
25 |
6.1 |
2.6 |
6 |
20.9 |
8.0 |
1000 |
250 |
6.3 |
2.8 |
6 |
20.9 |
7.9 |
|
10000 |
2500 |
5.8 |
2.98 |
6 |
21.0 |
7.8 |
|
Control 1 |
|
|
6.1 |
2.9 |
6 |
21.0 |
7.9 |
Control 2 |
|
|
6.2 |
2.6 |
6 |
20.9 |
8.0 |
Physio-chemical oxygen consumption control |
10000 |
2500 |
9.2 |
9.2 |
9 |
21.0 |
7.5 |
Reference substance |
5 |
2.5 |
6.0 |
3.1 |
6 |
21.0 |
8.0 |
10 |
5 |
6.4 |
4.5 |
5 |
20.9 |
8.0 |
|
20 |
10 |
8.6 |
7.8 |
7 |
20.9 |
8.1 |
Comments: Because of strong respiration of the activated sludge only 0.32 g/l ss were used.
The test substance has been added to about 130ml deionised water and stirred overnight before testing (equilibration phase).
* Volume added in ml of 3.5-Dichlorophenol stock solution (500 mg/l), total volume 250 ml.
STUDY EVALUATION
Test Substance: T-9601
Reference substance: 3.5-Dichlorophenol
|
Test concentration [mg/l] |
Respiratory rate [mg/l h] |
Phys. chem. O2-consumption [mg/l h] |
Respiratory rate- phys. chem. O2-consumption [mg/l h] |
Inhibition [%] |
Test substance |
100 |
35.0 |
0.0 * |
35.0 |
0.0 |
1000 |
35.0 |
0.0 * |
35.0 |
0.0 |
|
10000 |
29.0 |
0.0 |
29.0 |
14.7 |
|
Control, mean |
|
34.0 |
|
||
Control 1 |
36.0 |
||||
Control 2 |
36.0 |
||||
Reference substance |
5 |
29.0 |
- |
- |
14.7 |
10 |
22.8 |
- |
- |
32.9 |
|
20 |
6.9 |
- |
- |
79.7 |
Comments relevant to result:
Purity of test substance: 94.7%
Purity of the reference substance: 97%
Test concentrations are given as nominal concentrations and have not been confirmed by analytical methods.
* The physico-chemical oxygen consumption is determined at 10000 mg/l test substance. No physic-chemical oxygen consumption has been determined. Therefore lower concentrations of the test substance cause no physic-chemical oxygen consumption (deduced values).
Description of key information
Toxicity of T-9601 to activated sludge: EC 50 > 10000 mg/L
Key value for chemical safety assessment
- EC50 for microorganisms:
- 10 000 mg/L
Additional information
Study conducted to Commission Directive 88/302/EEC; Official Journal of the EC L 133, Part C: Biodegradability: Test for inhibition of oxygen consumption (corresponds for the most part to the test method OECD 209). Study in compliance with GLP.
A defined quantity of activated sludge is mixed with synthetic medium and the respiratory rate is measured. This rate is compared to those measured in test preparations with various concentrations of the test substance. In order to check the sensitivity of the activated sludge used, 3.5-dichlorophenol is used as a reference substance.
Toxicity of T-9601 to activated sludge : EC 50 > 10000 mg/L
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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