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Diss Factsheets
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EC number: 604-714-9 | CAS number: 149968-48-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study (OECD)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 006
- Report date:
- 2006
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- N-[3-dimethylamino)propyl]docosonamide, lactic acid salt
- IUPAC Name:
- N-[3-dimethylamino)propyl]docosonamide, lactic acid salt
- Test material form:
- not specified
- Details on test material:
- - Name of test material (as cited in study report): APA-220
- Molecular weight (if other than submission substance): 514.8 g/mol
- Structural formula attached as image file (if other than submission substance): see Figure 1
- Physical state: white solid
- Analytical purity: 99.2% (w/w)
- Impurities (identity and concentrations): behenic acid (0.6%), water (0.2%)
- Stability under test conditions: stable in water, and other solvent, to heat and light etc.
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Strain: no data
- Source: University of Sheffield (Sheffield S10 2UQ, United Kingdom)
- Age at study initiation: less than 24-hour old daphnids were used for the study
- Feeding during test: test organisms were not fed during the exposure
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Test temperature:
- 20±1°C
- pH:
- 7.9
- Dissolved oxygen:
- At least 60% or more of the saturate concentration at the water temperature
- Nominal and measured concentrations:
- Definitive test - Nominal test concentration of the test media: 0.313 - 0.625 - 1.25 - 2.50 - 5.00 - 10.0 mg/L
Definitive test - Geometric mean concentration of the untreated test media: 0.245 - 0.497 - 0.999 - 1.99 - 3.74 - 7.82 mg/L
Definitive test - Geometric mean concentration of the centrifugated test media: 0.194 - 0.390 - 0.775 - 1.52 - 2.04 - 6.38 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 mL glass beaker
- Type: closed
- Aeration: no
- Renewal rate of test solution: test solutions renewed at 24 hours after the start of the exposure
- No. of organisms per vessel: 20
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted test water (ISO 6341)
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16-hour light/8-hour dark photoperiod daily with room lamp
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.0 (geometrical series)
- Range finding study
- Test concentrations:
- Results used to determine the conditions for the definitive study: the test concentrations and the factor were decided based on the results of preliminary tests - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 1.41 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- act. ingr.
- Remarks:
- (untreated test media)
- Basis for effect:
- mobility
- Remarks on result:
- other: (95% confidence limits: 1.12-1.76)
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 1.09 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- act. ingr.
- Remarks:
- (centrifugated test media)
- Basis for effect:
- mobility
- Remarks on result:
- other: (95% confidence limits: 0.871-1.36)
- Details on results:
- - Mortality of control: 0%
- Abnormal responses: no abnormal response observed in the control - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- EC50 (48h): 0.262 mg/L
- Other: test conducted in May 2006
Any other information on results incl. tables
Table 1. Immobility
Geometric mean of the measured concentration (mg/L) |
Mean % immobility (after 48 hours of exposure) |
Control |
0 |
0.245 (0.194) |
0 |
0.497 (0.390) |
0 |
0.999 (0.775) |
40 |
1.99 (1.52) |
70 |
3.74 (2.94) |
90 |
7.82 (6.38) |
100 |
The value in parenthesis was geometric mean of the measured concentrations by treatment of centrifugation (10,000 g, 30 min.).
Table 2. Observed abnormal response
Geometric mean of the measured concentration (mg/L) |
Observed abnormal response (after 48 hours of exposure) |
Control |
No abnormal response |
0.245 |
No abnormal response |
0.497 |
No abnormal response |
0.999 |
IM LETH RA |
1.99 |
IM LETH RA |
3.74 |
IM LETH RA |
7.82 |
IM LETH |
Abbreviation of symptoms
IM: Immobilization
LETH: Lethargic
RA: Reduced activity
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- According to a GLP-compliant guideline study (OECD 202), the test material (N-[3-(dimethylamino)propyl]docosonamide, lactic acid salt) is toxic to aquatic invertebrates (D. magna: EC50 (48h) = 1.09-1.41 mg/L; based on geometric mean measured test concentrations).
- Executive summary:
A study was performed to assess the effect of the test material on the mobility of the water flea Daphnia magna. The study followed GLP and was performed according to the OECD Guideline for Testing of Chemicals, Section 2 : Effects on biotic systems, 202 "Daphnia sp., Acute Immobilisation Test (Guideline 202, April 13, 2004)".
Following preliminary tests, Daphnia magna was exposed to an aqueous dispersion of the test material at nominal concentrations of 0.313, 0.625,1.25, 2.50, 5.00 and 10.0 mg/L (four replicates per test concentration) for 48 hours, under 16-hour light/8-hour dark photoperiod at a temperature of 20±1°C. The concentrations of test solutions were measured both in dispersed condition (no treatment) and in dissolved condition (treatment by centrifugation [10,000 g, 30 min.]).
Based on the geometric mean measured test concentration of the untreated test media, the EC50 (48h) value was 1.41 mg/L (95% CL: 1.12 -1.76 mg/L). Based on the geometric mean measured test concentration of the centrifugated test media, the EC50 (48h) value was 1.09 mg/L (95% CL: 0.871 -1.36 mg/L).
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