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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
toxicity to reproduction: other studies
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
3 (not reliable)
Rationale for reliability incl. deficiencies:
other: Significant methodological deficiences.

Data source

Reference
Reference Type:
publication
Title:
Toxicity of rubber chemicals towards three-day chicken embryos.
Author:
Korhonen A, Hemminki K, Vainio H.
Year:
1983
Bibliographic source:
Scand-J-Work-Health, 9 : 115-119.

Materials and methods

Test guideline
Qualifier:
no guideline available
Principles of method if other than guideline:
Three­day chicken embryos were exposed to DTDM.
GLP compliance:
not specified
Type of method:
other: Chicken embryos

Test material

Constituent 1
Chemical structure
Reference substance name:
Di(morpholin-4-yl) disulphide
EC Number:
203-103-0
EC Name:
Di(morpholin-4-yl) disulphide
Cas Number:
103-34-4
Molecular formula:
C8H16N2O2S2
IUPAC Name:
4-(morpholin-4-yldisulfanyl)morpholine

Test animals

Species:
other: Chicken embryos
Strain:
other: White Leghorn
Details on test animals or test system and environmental conditions:
Chicken eggs were obtained from the hatchery of Siipikarjanhoitajain Liito ry. Hämeenlinna, Finland.
Age : 3 days embryos.

Administration / exposure

Route of administration:
other: injection
Vehicle:
other: water or acetone (not precised for DTDM)
Details on exposure:
3 days old White Leghorn chicken embryos were injected by dropping the test substance (0.06-0.96 umol/egg) into the air chamber of the egg (20-30 eggs/dose); 10 control eggs were injected with the vehicle (5 µl acetone) only.
Analytical verification of doses or concentrations:
not specified
Duration of treatment / exposure:
3 days
Frequency of treatment:
continuously
Duration of test:
11 days after the treatment (on day 14)
No. of animals per sex per dose:
20-30 eggs/dose
Control animals:
yes, concurrent vehicle
Details on study design:
no
Statistics:
no

Results and discussion

Effect levels

Dose descriptor:
other: ED50
Effect level:
0.09 other: µmol/egg
Basis for effect level:
other: concentration that induced malformations or death in 50 % of the embryos

Observed effects

no data

Any other information on results incl. tables

Table : Median effective doses (ED50) and the slopes of the dose-response curves for total effect consisting of deaths and malformations caused by DTDM.

Chemical

Total effect

Maximum percentage of malformed embryos (treated)

ED50 (dose/egg)

(tan alpha)

DTDM

0.09 µmol

1.2

67

Applicant's summary and conclusion

Conclusions:
The ED50 (concentration that induced malformations or death in 50 % of the embryos) of DTDM was 0.09 µmol /egg.
Executive summary:

Three­day chicken embryos were exposed to DTDM dissolved in either acetone or water. Dose-response curves and median effective doses (ED50) were calculated for mortality in 2 days after the treatment, for total mortality in 11 days after the treatment, and for the total effect, including deaths and malformations 11 days after the treatment, when the test was finished. Sixteen of the chemicals had no effect on the embryos when injected into the air chamber.