Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 413-010-9 | CAS number: 6613-44-1 DMBC
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 13 October 1992 - 8 March 1993
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study conducted to GLP in accordance with generally accepted scientific principles, possibly with incomplete reporting or methodological deficiencies, which do not affect the quality of the relevant results.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 993
- Report date:
- 1993
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Qualifier:
- according to guideline
- Guideline:
- EPA OPP 84-2
- Principles of method if other than guideline:
- Only 4 strains of Salmonella typhimurium were tested.
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 3,5-dimethylbenzoyl chloride
- EC Number:
- 413-010-9
- EC Name:
- 3,5-dimethylbenzoyl chloride
- Cas Number:
- 6613-44-1
- Molecular formula:
- C9H9ClO
- IUPAC Name:
- 3,5-dimethylbenzoyl chloride
- Details on test material:
- DMBC
Constituent 1
Method
- Target gene:
- S. typhimurium Histidine locus
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Details on mammalian cell type (if applicable):
- Strains were characterised for nutritional requirements, crystal violet sensitivity, and ampicillin resistance no more than 6 months prior to initiation of the study.
- Metabolic activation:
- with and without
- Metabolic activation system:
- S-9 derived from the livers of rats induced with Aroclor 1254.
- Test concentrations with justification for top dose:
- Definitive Assay: 0, 50, 200, 500, 2000 and 5000 µg/plate.
Confirmatory Assay: 0, 160, 300, 500, 900 and 1600 µg/plate.
All concentrations were adjusted for active ingredient. - Vehicle / solvent:
- Acetone
Controlsopen allclose all
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 2-anthramine
- Remarks:
- In the presence of metabolic activation for all 4 strains
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 2-nitrofluorene
- Remarks:
- In the absence of metabolic activation for TA98.
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- Remarks:
- In the absence of metabolic activation for TA100 and TA1535
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- Remarks:
- In the absence of metabolic activation for TA1537
- Details on test system and experimental conditions:
- Metabolic Activation
The S-9 used for metabolic activation was obtained from rats induced with Aroclor 1254. The S-9 mix consisted of the following:
Nicotinamide-adenine dinucleotide phosphate (NADP) 4 mM
Glucose-6-phosphate 5 mM
Magnesium chloride (MgCl2) 8 mM
Potassium chloride (KCl) 33 mM
Sodium phosphate buffer, pH 7.4 100 mM
Liver homogenate (S-9) from Aroclor 1254 induced rats 10 %
The mix used in the non-activated portion of the assay consisted of the above with equal amounts of saline substituted for NADP and S-9.
Mutagenesis Assay
The tester strains were sub-cultured in Oxoid Nutrient Broth #2 for approximately 10 h at 37 ± 1 degrees Celsius. The fresh inoculum was used when bacterial growth was approximately 10^8 to 10^9 cells per mL.
Control plates were run to check for sterility, to determine the background reversion rate, and to measure the response of each tester strain to a positive control compound.
For the activated portion of the assay the following were added, in order, to sterile test tubes: 2 mL of top agar, 0.1 mL of the bacteria inoculum, 0.1 mL of the appropriate concentration of test compound, and 0.5 mL of phosphate buffer mix (with S-9 and NADP). For the non-activated portion of the assay, the above procedure was followed, except that the 0.5 mL of phosphate buffer mix (without S-9 or NADP) was added to the tubes directly after addition of the top agar. Each test article concentration was tested in triplicate, in minimal plates (minimal-glucose agar medium). The controls were tested in six replicates in minimal plates. The contents of the tubes were mixed and poured onto petri dishes containing approximately 19 mL of the appropriate agar. Plates were allowed to set for several minutes then placed in covered plastic boxes and incubated at 37 ± 1 degrees Celsius for approximately 72 hours prior to colony counting. Results were confirmed in an independent assay. - Evaluation criteria:
- Following the incubation period, sterility plates were checked for contamination. Following the sterility check, the number of colonies on each plate was determined. The mean and standard deviation for each concentration was calculated. Background growth was checked for each experimental point to observe any toxic response.
A mutagenicity assay is considered valid if the following conditions are met. First, the spontaneous reversion rate, with and without metabolic activation, must be reasonably consistent with the expected range for the strain being used; i.e., 12 - 60 colonies per plate for TA98, 50 - 150 for TA100, 13 - 50 for TA1535, and 7 - 20 for TA1537. Second, the positive control materials must elicit a positive response. And third, strains must have maintained characteristics, i.e., nutritional requirements, crystal violet sensitivity and ampicillin resistance.
A test article is considered positive (mutagenic) if it elicits in independent assays a number of revertants per plate at least 2 times that observed in the solvent control (background). A response that does not meet this criteria but elicits a potential biologically significant response (e.g., a dose related increase in revertants per plate over 3 concentrations) is considered an equivocal response and requires further evaluation.
A test article is considered negative (non-mutagenic) if the criteria for a positive assay were not met and the test article was tested up to 5000 µg/plate or the limit of solubility or toxicity, whichever was lower. Toxicity is defined as the elimination of a uniform background lawn. - Statistics:
- Statistical methods beyond the calculation of the mean and standard deviation are not considered necessary for the interpretation of this study.
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Additional information on results:
- In the definitive assay, the test article was evaluated at doses of 50, 200, 500, 2000, and 5000 µg/plate (all concentrations adjusted for active ingredient) in the presence and absence of S-9 (see Table 1 for mean summary data).
The study was designed to evaluate the mutagenic potential of the test article up to the limits of solubility, toxicity, or 5000 µg/plate (whichever was lower). A precipitate was observed in all strains with metabolic activation at doses of 2000 µg/plate and greater, and in TA98 and TA1535 without metabolic activation at 2000 µg/plate. Precipitate and toxicity were observed in all strains without metabolic activation, at 5000 µg/plate and also at 2000 µg/plate in strain TA1537.
An independent confirmatory assay was performed at lower concentrations ranging from 160, 300, 500, 900 and 1600 µg/plate (see Table 2 for mean summary data). A precipitate was observed in strains TA98, TA1535 and TA1537 at 1600 µg/plate with metabolic activation, and in TA98 and TA1537 without metabolic activation. A mutagenic response was not detected in the tester strains in any of the experiments conducted.
At test article concentrations not exhibiting precipitate or toxicity, a mutagenic response was not detected in any of the Salmonella tester strains examined. - Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Table 1 Definitive Assay Mean Summary Data
Data reported as: Mean (Standard Deviation)
Controls |
|||||
|
Mean Revertants/Plate |
||||
S-9 |
TA98 |
TA100 |
TA1535 |
TA1537 |
|
Solvent Controls Acetone Acetone |
+ - |
24 (4) 40 (7) |
125 (18) 126 (12) |
14 (4) 17 (5) |
8 (2) 13 (4) |
Positive Controls 2-anthramine 2 µg/plate 2-nitrofluorine 3 µg/plate Sodium azide 2 µg/plate 9-aminoacridine 100 µg/plate |
+ - - - |
766 (113) * 377 (58)* |
1314 (74)**
754 (55) * |
134 (6) *
633 (53)* |
86 (42)*
109 (66)* |
Test Compound |
|||||
Dose Level (µg/plate) |
Mean Total Revertant Colonies/Plate |
||||
S-9 |
TA98 |
TA100 |
TA1535 |
TA1537 |
|
5000 2000 500 200 50 5000 2000 500 200 50 |
+ + + + + - - - - - |
-† -† 27 (6) 20 (3) 19 (2) -§ -† 25 (12) 36 (13) 37 (2) |
-† 92 (30)‡ 121 (29) 101 (9) 124 (21) -§ 63 (12) 102 (24) 108 (10) 126 (12) |
-† -† 18 (8) 19 (2) 15 (3) -§ -† 11 (2) 19 (1) 17 (3) |
-† -† 2 (0) 8 (3) 9 (1) -§ -§ 7 (0) 14 (1) 17 (6) |
*Positive response: ≥2 x solvent
**Misdosed
†Precipitate on plate
‡Precipitate on plate (1 out of 3)
§Toxicity and precipitate
**†‡§Observations excluded from calculations.
Table 2 Confirmatory Assay Mean Summary Data
Data reported as: Mean (Standard Deviation)
Controls |
|||||
|
Mean Revertants/Plate |
||||
S-9 |
TA98 |
TA100 |
TA1535 |
TA1537 |
|
Solvent Controls Acetone Acetone |
+ - |
21 (3) 32 (6) |
177 (6)# 184 (7)# |
24 (4) 20 (4) |
8 (2) 19 (1) |
Positive Controls 2-anthramine 2 µg/plate 2-nitrofluorine 3 µg/plate Sodium azide 2 µg/plate 9-aminoacridine 100 µg/plate |
+ - - - |
961 (121)* 698 (154)* |
1343 (116)*
669 (48)* |
183 (30)
602 (64)* |
121 938)*
143 (43) |
Test Compound |
|||||
Dose Level (µg/plate) |
Mean Total Revertant Colonies/Plate |
||||
S-9 |
TA98 |
TA100 |
TA1535 |
TA1537 |
|
1600 900 500 300 160 1600 900 500 300 160 |
+ + + + + - - - - - |
-† 21 (7) 28 (7) 24 (7) 34 (8) -† 19 (1) 23 (10) 33 (9) 24 (4) |
125 (43) 141 (13) 135 (18) 168 (11) 166 (20) 103 (20) 142 (8) 166 (1) 172 (10) 178 (20) |
-† 11 (5) 15 (2) 20 (6) 16 (5) 13 (4) 17 (5) 14 (3) 17 (1) 23 (3) |
-† 6 (2) 4 (2) 7 (3) 9 (2) -† 11 (3) 21 (5) 20 (4) 22 (6) |
#Outside of historical database (3.0 Standard Deviations) (Due to a limited database: n = 6)
*Positive response: ≥2 x solvent
†Precipitate on plate. Observations excluded from calculations.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
Under the conditions of this study, DMBC is not mutagenic in the Salmonella, gene mutation assay. - Executive summary:
DMBC was evaluated for mutagenic activity in the Salmonella typhimurium gene mutation assay (Ames test) in accordance with EPA 40 CFR Part 158.340 Guideline 84-2 and OECD Guideline 471.
Tester strains were TA98, TA100, TA1535, and TA1537 ± S-9. The test article was evaluated in a definitve assay at concentrations ranging from 50 to 5000 µg/plate (all concentrations adjusted for active ingredient) and the number of revertants was determined. An independent confirmatory assay was performed using doses ranging from 160 to 1600 µg/plate
The test article did not induce an increase in revertants when compared to solvent controls. This was true for all tester strains both with and without metabolic activation. Toxicity and/or precipitation of the test article were observed at concentrations of 2000 to 5000 µg/plate in the definitive assay. Precipitation of the test article was observed at a dose of 1600 µg/plate in strains TA98, TA1535 and TA1537 but not in TA100.
Under the conditions of this study, DMBC is not mutagenic in the Salmonella gene mutation assay.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.