2,5-dimethylhexyne-2,5-diol

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Study performed according to OECD guideline and in compliance with GLP criteria.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1998

Report date:

1998

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

2,5-dimethylhexyne-2,5-diol

EC Number:

205-533-4

EC Name:

2,5-dimethylhexyne-2,5-diol

Cas Number:

142-30-3

Molecular formula:

C8H14O2

IUPAC Name:

2,5-dimethylhex-3-yne-2,5-diol

Details on test material:

- Name of test material (as cited in study report): 2,5-Dimethyl-3-hexin-2,5-diol
- Physical state: white homogeneous solid

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Test substance analysis was performed for nominal concentrations of 0, 12.5 and 100 mg/L. Samples were stored in a refrigerator at 4°C until analysis.

Test solutions

Vehicle:

no

Details on test solutions:

The test substance was stirred in M4-medium for about 10 minutes at 22±1°C to obtain a stock solution of 100 mg/L (nominal). The stock solution was further diluted with water to obtain the required test substance concentrations.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Strain: STRAUS
- Source: Institut National de Recherche Chimique Appliquee, France. Cultured under standard conditions in the Laboratory of Ecotoxicology BASF AG (Ludwigshafen) since the beginning of 1978.
- Age at study initiation: 2-24 hours

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

2.47 mmol/L

Test temperature:

20-21.2°C

pH:

7.9-8.0

Dissolved oxygen:

8.3-8.5 mg/L

Nominal and measured concentrations:

- Nominal: 0 (control), 12.5, 25, 50 and 100 mg/L
- Measured: >80% recovery.

Details on test conditions:

TEST SYSTEM
- Test vessel: 20 mL test tubes containing 10 mL test medium
- Type: open
- Aeration: yes,
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 0.5/mL

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: day:night = 16h:8h
- Light intensity: ca. 5 - 6 µE/(m²·s) at a wavelength rate of 400-700 nm

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

Results are expressed as nominal concentrations as mean measured concentrations were >80% of nominal.