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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in mammalian cells
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: In spite of a lack of some information, the study is comparable to guideline study with acceptable restrictions

Data source

Reference
Reference Type:
publication
Title:
Genetic toxicology of lead compounds
Author:
J. T. Zelikoff, J. H. Li, A. Hartwig, X. W. Wang, M. Costa and T. G. Rossman
Year:
1988
Bibliographic source:
Carcinogenesis vol.9 n°10 pp. 1727-1732, 1988

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 476 (In Vitro Mammalian Cell Gene Mutation Test)
Deviations:
yes
Qualifier:
according to guideline
Guideline:
OECD Guideline 479 (Genetic Toxicology: In Vitro Sister Chromatid Exchange Assay in Mammalian Cells)
Deviations:
yes
GLP compliance:
not specified
Type of assay:
mammalian cell gene mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
Lead sulphide
EC Number:
215-246-6
EC Name:
Lead sulphide
Cas Number:
1314-87-0
Molecular formula:
PbS
Constituent 2
Reference substance name:
Lead sulfide
IUPAC Name:
Lead sulfide

Method

Species / strain
Species / strain / cell type:
Chinese hamster lung fibroblasts (V79)
Details on mammalian cell type (if applicable):
- Type and identity of media: Ham's F12 media supplemented with 10% heat-inactivated Fetal Bovine Serum
Additional strain / cell type characteristics:
not specified
Metabolic activation:
not specified
Controls
Untreated negative controls:
not specified
Negative solvent / vehicle controls:
not specified
True negative controls:
not specified
Positive controls:
yes
Positive control substance:
other: N-methyl-N'-nitro-N-nitrosoguanidine
Remarks:
0.5µg/mL
Details on test system and experimental conditions:
METHOD OF APPLICATION: in F12 medium

DURATION
- Preincubation period: 24H
- Exposure duration: 24H
- Expression time (cells in growth medium):
- Selection time (if incubation with a selection agent): 2
- Fixation time (start of exposure up to fixation or harvest of cells): 10Days

NUMBER OF CELLS EVALUATED: 500
Evaluation criteria:
clonal survival

Results and discussion

Test results
Species / strain:
Chinese hamster lung fibroblasts (V79)
Metabolic activation:
without
Genotoxicity:
positive
Cytotoxicity / choice of top concentrations:
not specified
Vehicle controls validity:
not specified
Untreated negative controls validity:
not specified
Positive controls validity:
not specified
Remarks on result:
other: strain/cell type: HPRT
Remarks:
Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information):
positive

Under the conditions of the study lead sulphide was found mutagenic at the HPRT locus, in V79 cells, at concentrations that do not induce SCE.