Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
other: Although less details on test conditions and on analytical method endpoint results are used for classifation
Rationale for reliability incl. deficiencies:
other: Not enough details on test conditions, no analytical method.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1983
Report Date:
1983

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
other: AFNOR norm T 90301 of april 1974, modified according to AFNOR doc T 95 B for reconstitued river water
Deviations:
not specified
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Details on test material:
o-TFMA used was the commercial quality.

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
Preparation and application of test solution:
The test substance was diluted in bidistilled water at a concentration slightly higher than expected toxicity treshold.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
no data

Study design

Test type:
not specified
Water media type:
freshwater
Limit test:
no
Total exposure duration:
24 h

Test conditions

Details on test conditions:
WATER PARAMETERS
- Source/preparation of dilution water: reconstituted river water

Other test conditions: no data
Reference substance (positive control):
no
Remarks:
preliminary tests were done to determine level of toxicity in the main test

Results and discussion

Effect concentrations
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
10 mg/L
Nominal / measured:
not specified
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95% CL: 8-12 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
The EC50 24h was 10 mg/L based on mobility. 
Therefore, the EC50 48h is between 1 and 10 mg/l, and the substance considered as toxic to aquatic organisms.

Since the substance is not readily biodegradable it is classified as:

Directive 67/548/EEC: R51/R53
Regulation (EC) No. 1272/2008: H411
Executive summary:

The ortho-trifluoromethylaniline has been tested for acute toxicity to Daphnia magna Strauss (strain IRCHA) according to the AFNOR norm T 90301 of april 1974.

The daphnids, obtained from the laboratory breeding, were assayed during 24 hours, in the dark, at 20 +/-1 °C, in a range of substance concentrations prepared in the assay medium (20 daphnids per concentration).

The EC50 24h was 10 mg/L based on mobility.

 

Therefore, the EC50 48h is between 1 and 10 mg/l, and the substance considered as toxic to aquatic organisms.