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EC number: 939-717-7 | CAS number: 1474044-79-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
The oral LD50 is > 2500 mg/kg.
The dermal LD50 is > 10,000 mg/kg.
The use as additive is unlikely to result in significant human exposure
to inhalable droplets. In addition, a 1 hour exposure at 9 mg/L did not
cause mortality.
Key value for chemical safety assessment
Acute toxicity: via oral route
Link to relevant study records
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- February - April 1978
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Methods generally follow accepted procedures for acute oral toxicity studies. The number of animals tested (10) exceed current requirements and the result is considered statistically valid.
- Qualifier:
- according to guideline
- Guideline:
- other: FHSLA, CFR Title 21, para. 191.1
- Deviations:
- not specified
- GLP compliance:
- no
- Test type:
- other:
- Limit test:
- yes
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- The rats were fasted for 18 hours and then individually and singly dosed by gavage
with 5 ml /kg body weight of test material. The rats were individually caged and observed
for mortal ity or other signs of gross toxicity for 14 days. Food and water were provided ad libitum. - Route of administration:
- oral: gavage
- Vehicle:
- unchanged (no vehicle)
- Doses:
- Limit dose of 5000 ml/kg body weight
- No. of animals per sex per dose:
- 5 males and 5 females.
- Control animals:
- no
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 5 mL/kg bw
- Based on:
- test mat.
- Mortality:
- One male rat died on day 1.
- Clinical signs:
- other: All animals appeared active and healthy.
- Gross pathology:
- No remarkable findings for animal 63 that died on day 1.
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- The rats were fasted for 18 hours and then individually and singly dosed by gavage
with 5 ml /kg body weight of test material. The rats were individually caged and observed
for mortal ity or other signs of gross toxicity for 14 days. Food and water were provided ad libitum.
The test material is not toxic at a single oral dose of 5ml/kg. The oral LD50 is > 5g/kg. On an active ingredient basis, the LD50 is > 2.5 mg/kg. - Executive summary:
An oral LD50 was conducted with NASUL 729, 50% Calcium DNNSA (CAS 57855-77-3). Five male and five female Sprague Dawley derived albino rats were fasted for 18 hours and then individually and singly dosed by gavage with 5 ml /kg body weight of test material. No clinical symptoms were reported. One male rat died on day 1. The test material was considered not toxic at a single oral dose of 5ml/kg. The oral LD50 is > 5g/kg. On an active ingredient basis, the LD50 is > 2.5 mg/kg.
Reference
Rat-No. |
Sex |
BodyWeight-Initial grams |
Dose ml |
Death Day |
63 |
M |
210 |
1.05 |
1 |
64 |
M |
200 |
1.0 |
Survived |
65 |
M |
225 |
1.12 |
Survived |
66 |
M |
205 |
1.02 |
Survived |
67 |
M |
200 |
1.0 |
Survived |
47 |
F |
228 |
1.14 |
Survived |
48 |
F |
210 |
1.05 |
Survived |
49 |
F |
213 |
1.06 |
Survived |
50 |
F |
219 |
1.10 |
Survived |
51 |
F |
200 |
1.0 |
Survived |
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed
- Dose descriptor:
- discriminating dose
- Value:
- 2 500 mg/kg bw
Acute toxicity: via inhalation route
Link to relevant study records
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: An appropriate number of animals were exposed to a concentration (~18 mg/L) greatly exceeding the standard limit concentration (5 mg/L).
- Qualifier:
- according to guideline
- Guideline:
- other: FHSLA, CFR, Title 21 J para. 191.10.
- Deviations:
- not specified
- GLP compliance:
- no
- Test type:
- fixed concentration procedure
- Limit test:
- yes
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- A group of five male and five female Wistar rats were exposed to the test
material. - Route of administration:
- inhalation: vapour
- Type of inhalation exposure:
- whole body
- Vehicle:
- not specified
- Details on inhalation exposure:
- Rats were exposed to NASUL 729 (vaporized) in an inhalation chamber for 1 hour. The chamber was saturated with
18 mg/liter nominal of the test material prior to the period of exposure.
The rats were observed for 14 days following exposure. Feed and water were provided ad libitum. - Analytical verification of test atmosphere concentrations:
- no
- Duration of exposure:
- ca. 1 h
- Concentrations:
- 18 mg/liter nominal
- No. of animals per sex per dose:
- 5 male and 5 female rats
- Control animals:
- no
- Statistics:
- None required. No mortality.
- Sex:
- male/female
- Dose descriptor:
- LC50
- Effect level:
- > 18 mg/L air (nominal)
- Based on:
- test mat.
- Exp. duration:
- 1 h
- Mortality:
- No mortality.
- Clinical signs:
- other: No untoward reactions.
- Body weight:
- See table.
- Gross pathology:
- None reported.
- Interpretation of results:
- other: relatively harmless
- Conclusions:
- The Inhalation LC50 of rats exposed for 1 hour to the test material is greater than 18 mg/liter.
- Executive summary:
A group of five male and five female Wistar rats were exposed to NASUL 729. The rats were exposed to NASUL 729 (vaporized) in an inhalation chamber for 1 hour. The chamber was saturated with 18 mg/liter nominal of the test material prior to the period of exposure. The rats were observed for 14 days following exposure. Feed and water were provided ad libitum. No mortalities or clinical symptoms observed. The Inhalation LC50 of rats exposed for 1 hour to the test material is greater than 18 mg/liter. On an active ingredient basis the LC50 > 9 mg/L.
Reference
Rat No. |
Sex |
Body Weight - grams |
Mortality |
1 |
M |
229 |
No |
2 |
M |
265 |
No |
3 |
M |
229 |
No |
4 |
M |
256 |
No |
5 |
M |
263 |
No |
6 |
F |
291 |
No |
7 |
F |
271 |
No |
8 |
F |
256 |
No |
9 |
F |
261 |
No |
10 |
F |
278 |
No |
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed
- Dose descriptor:
- discriminating conc.
- Value:
- 9 000 mg/m³ air
Acute toxicity: via dermal route
Link to relevant study records
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Methods generally follow accepted procedures for acute dermal toxicity studies. The applied dose exceeds current requirements and the result is considered valid.
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- other: FHSLA, CFR. Title 21. para. 191.10 and Appraisal of the Safety of 'Chemicals in Foods, Drugs and Cosmetics, Association of Food and Drug Officials of the U.S.
- GLP compliance:
- no
- Test type:
- other: limit test
- Limit test:
- yes
- Species:
- rabbit
- Strain:
- New Zealand White
- Sex:
- male/female
- Type of coverage:
- occlusive
- Vehicle:
- unchanged (no vehicle)
- Details on dermal exposure:
- Five male and 5 female rabbits were prepared by cl ipping the skin of the trunk
free of hair. Epidermal abrasions were made over a 5-6 cm2 area on 2 males and 3
females. A patch containing 20 g/kg body weight of the test material was placed over a
5-6 cm2 area on all rabbits and secured with an elastic sleeve. The rabbits were immobil ized
in head stocks for 24 hours at which time the patches were removed and the rabbits returned
to their cages. Feed and water were provided ad libitum. The rabbits for observed for 14 days. - Duration of exposure:
- 24 hours
- Doses:
- 20 g/kg bw
- No. of animals per sex per dose:
- 5
- Control animals:
- no
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 20 other: g / kg bw
- Mortality:
- No mortality.
- Clinical signs:
- other: Anorexia - first 5 days, some weight loss, reversed after 9 days.
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- The dermal LD50 > 20g/kg. The dermal LD50 on an active ingredient basis is > 10 g/kg (10,000 mg/kg).
- Executive summary:
A dermal acute toxicity study was conducted with NASUL 729. This formulation contains 50% Calcium DNNSA.
Five male and 5 female rabbits were prepared by cl ipping the skin of the trunk free of hair. Epidermal abrasions were made over a 5-6 cm2 area on 2 males and 3 females. A patch containing 20 g/kg body weight of the test material was placed over a 5-6 cm2 area on all rabbits and secured with an elastic sleeve. The rabbits were immobil ized in head stocks for 24 hours at which time the patches were removed and the rabbits returned to their cages. Feed and water were provided ad libitum. The rabbits for observed for 14 days.
No mortalities occurred. Anorexia was reported for the first 5 days, some weight loss occurred but reversed after 9 days. NASUL 729 is practically non-toxic by dermal exposure.
Reference
Rabbit-No. |
Sex |
BodyWeight kg |
Dose grams |
Mortality - Day - |
1-abraded |
M |
3.0 |
60 |
Survived |
2-abraded |
M |
3.0 |
60 |
Survived |
3 |
M |
2.92 |
59 |
Survived |
4 |
M |
2.88 |
57 |
Survived |
5 |
M |
2.92 |
58 |
Survived |
6-abraded |
F |
2.33 |
47 |
Survived |
7-abraded |
F |
2.46 |
49 |
Survived |
8-abraded |
F |
2.60 |
52 |
Survived |
9 |
F |
2.75 |
55 |
Survived |
10 |
F |
2.68 |
54 |
Survived |
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed
- Dose descriptor:
- discriminating dose
- Value:
- 10 000 mg/kg bw
Additional information
Justification for selection of acute toxicity – oral endpoint
An oral LD50 was conducted with NASUL 729, a 50% formulation of
Calcium DNNSA. No clinical symptoms were reported. One male rat died on
day 1. The oral LD50 is > 5000 mg/kg. On an active ingredient basis, the
LD50 is > 2500 mg/kg.
Justification for selection of acute toxicity – inhalation endpoint
The use as additive is unlikely to result in significant human
exposure to inhalable droplets.
Rats were exposed to NASUL 729 (vaporized) in an inhalation chamber for
1 hour. The chamber was saturated with
18 mg/liter nominal of the test material prior to the period of
exposure. The rats were observed for 14 days following exposure.
Justification for selection of acute toxicity – dermal endpoint
A dermal acute toxicity study was conducted with NASUL 729 at a high
dose of 20 grams/kg. This formulation contains 50% Calcium DNNSA.
Five male and 5 female rabbits were tested. Epidermal abrasions were
made over a 5-6 cm2 area on 2 males and 3 females to increase dermal
exposure.
Justification for classification or non-classification
Based on finding in acute oral, dermal and inhalative studies with the reference substance it can be concluded that no classification for acute toxicity by oral, dermal or inhalative route is required according to CLP (Regulation EC No 1272/2008) or DSD (Directive 67/548/EEC). Given the high viscosity and the fact that Ca-DNNSA is not a pure hydrocarbon, classification for aspiration hazards is not required. No specific target organ toxicity was observed in any of the acute studies and thus STOT single exposure classification is not required.
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