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EC number: 258-508-5 | CAS number: 53378-51-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- supporting study
- Justification for type of information:
- Please refer to Read Across Statement attached in Section 13
- Reason / purpose for cross-reference:
- read-across source
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 1 026 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 800 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 1 800 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- EC50: 1.7 mg/l which is not considered significantly different from the results obtained in an international ring test (EC50 = 1.0 - 2.2mg/l) - Validity criteria fulfilled:
- yes
- Remarks:
- Cell density increased by a factor of more than 16 during test, variation in pH < 1, results for reference substance within acceptable range, 1.7 (1.0-2.2) mg/L
- Conclusions:
- EC50(72 h) for the test substance is >1800 mg/L for Skeletonema costatum
- Executive summary:
The test was performed according to the ISO 10253 under GLP compliance. Skeletonema costatum was exposed to the test item for 72 hours. EC50(72 h) for the test substance is >1800 mg/L
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 08.10.2012 - 19.12.2013
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Qualifier:
- according to guideline
- Guideline:
- ISO 8692 (Water Quality - Fresh Water Algal Growth Inhibition Test with Scenedesmus subspicatus and Selenastrum capricornutum)
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- (Not the chemical analyses)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: (Nominal) 0 (control); 20; 50; 100; 200; 500; 1,000 and 2,000 mg/L
- Sampling method: Samples were collected at test start, every 24 hours and at the end of the test for chemical analysis of the test product. Duplicate samples of 15 mL were collected in 20-mL plastic vials for later analysis. Only one of these samples were analysed. The spare samples were discarded after sponsor approved the report.
- Sample storage conditions before analysis: at - 20 ˚C ± 2.0˚C - Vehicle:
- no
- Details on test solutions:
- A stock solution of 2,400 mg/L was prepared by weighing out 2,400 mg of the test product and dissolving it in 1 L of algal test medium. pH in the stock solutions was 9.3 and was therefore adjusted to 8.2 by adding 4 droplets of 1 M HCl. The test solutions were prepared by diluting the appropriate amounts of stock solution in algal test medium.
The test product was tested at the following nominal concentrations: 0 (control); 20; 50; 100; 200; 500; 1,000 and 2,000 mg/L. - Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
Clone: NIVA, CHL 1
The alga Pseudokirchnerella subcapitata is cultured at DHI under the test conditions prior to the start of the test. Algae from this culture were used in the study. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 23.2 ± 0.0˚C
- pH:
- 8.0-8.1 at the beginning and 7.8-10.2 at the termination of the test.
- Nominal and measured concentrations:
- The results from the analysis showed a measured IBP1-Na concentration of 88-102% of the estimated nominal concentration of IBP1-Na. As the tested concentraions were maintained within ± 20% of the nominal concentrations, the statistical calculaiton were based on the nominal concentrations.
- Details on test conditions:
- TEST SYSTEM
- Material, size, headspace, fill volume: 250 mL glass flasks with wide neck, containing 100 mL of test solution
- Renewal rate of test solution (frequency/flow rate):
- Initial cells density:
- Control end cells density:
- No. of organisms per vessel:
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6 (flasks with algae but no test product) + a blank of each concentration ( flasks with the test product but no algae)
Furthermore, one flask of the control and one flask of each test concentration without algae were used to cellect samples for chemical analysis.
OTHER TEST CONDITIONS
- Adjustment of pH: Test media: from 9.3 to 8.2 by adding 1 M HCl
- Photoperiod: 72 hours
- Light intensity and quality: fluorescent light with an intensity of approx 60-120 μmol x m-2 x sec-1
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: Beckman Coulter Multisizer and in-vivo fluorescence, Turner TD-700 Laboratory Fluorometer
TEST CONCENTRATIONS
- Test concentrations (nominal): 0 (control); 20; 50; 100; 200; 500; 1,000 and 2,000 mg/L. - Reference substance (positive control):
- yes
- Remarks:
- K2Cr2O7, CAS no 7778-50-9
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 261 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 245-278
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- EC50: 1.09 mg/L - Validity criteria fulfilled:
- yes
- Remarks:
- Growth rate = 1.9/day (> 1.4/day). Variation coefficient of control growth rates = 1.4 (< 5%). Mean varion coefficen = 13.4% (<35%). Increase of pH > 1.5 unit from start to end for control, not considered significant. EC50 for ref. sub. = 1.09 (0.65-1.73)
- Conclusions:
- ErC50(72h) of test item is 261 mg/L for Pseudokirchneriella subcapitata.
Remark: the test solution contains NaOH due to the production method. - Executive summary:
The toxicity to aquatic algae and cyanobacteria test was performed according to OECD Guideline 201 and ISO 8692 under GLP compliance. Pseudokirchneriella subcapitata, algae was exposed to the test item for 72 hours. ErC50(72h) of the test item value is determinded 261 mg/L.
Referenceopen allclose all
Description of key information
OECD 201, freshwater: ErC50(72h) of the test item value is determinded 261mg/L
Read-across, OECD 201, marine water: ErC50(72h) of the test item value is determinded 261mg/L, ErC10(72h) is determined 1026 mg/L
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 261 mg/L
- EC50 for marine water algae:
- 1 800 mg/L
- EC10 or NOEC for marine water algae:
- 1 026 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.