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Diss Factsheets
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EC number: 254-100-6 | CAS number: 38720-66-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 23 SEP 1982 to 27 OCT 1982
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Comparable to guideline study with acceptable restrictions (MMAD not calculated, no necropsy performed, no observation on the weekends, individual raw data not included in report, only 6 males/group)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 983
- Report date:
- 1983
Materials and methods
- Principles of method if other than guideline:
- - acute inhalation toxicity (4 h exposure)
- GLP compliance:
- no
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- 5,12-dihydroquino[2,3-b]acridine-7,14-dione
- EC Number:
- 213-879-2
- EC Name:
- 5,12-dihydroquino[2,3-b]acridine-7,14-dione
- Cas Number:
- 1047-16-1
- IUPAC Name:
- 5,12-dihydroquino[2,3-b]acridine-7,14-dione
Constituent 1
Test animals
- Species:
- rat
- Strain:
- other: Crl:CD
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Age at study initiation: 7 to 8 weeks
- Weight at study initiation: 226 to 250 g
- Housing: stainless steel wire mesh cages (8" x 8" x 14") in pairs
- Diet: Purina certified chow #5002; ad libitum
- Water: ad libitum
Administration / exposure
- Route of administration:
- inhalation: dust
- Type of inhalation exposure:
- head only
- Vehicle:
- other: unchanged (no vehicle)
- Details on inhalation exposure:
- GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- System of generating particulates: Dust atmosphere of the test item was generated with a vertical two-stage glass generator consisting of a dust reservoir and a cyclone elutriator. An electric steel motor and steel rod with plastic paddles agitated dust in the generator. Air introduced at the reservoir carried dust particles upward to the elutriator. Additional houseline air carried airborne dust into the chamber.
TEST ATMOSPHERE
- Brief description of analytical method used: Samples from chamber were analyzed at 30-minute intervals. Calibrated volumes of test atmosphere were passed through preweighed glass fiber filters (Gelman Type AE, 25 mm) and atmospheric concentrations determined from filter weight gain. Airborne particle size measurements were obtained using Sierra 8-stage cascade impactor.
TEST ATMOSPHERE (if not tabulated)
- Particle size distribution:
at concentration of 3.1 mg/L: 21 % have an aerodynamic diameter < 13 µm
at concentration of 2.6 mg/L: not determined
at concentration of 2.4 mg/L: not determined
at concentration of 1.6 mg/L: 17 % have an aerodynamic diameter < 13 µm
at concentration of 1.5 mg/L: 12 % have an aerodynamic diameter < 13 µm
- MMAD (Mass median aerodynamic diameter) / GSD (Geometric st. dev.): not calculated
- Analytical verification of test atmosphere concentrations:
- yes
- Remarks:
- gravimetric measurement
- Duration of exposure:
- 4 h
- Concentrations:
- 1.5, 1.6, 2.4, 2.6, 3.1 mg/L
- No. of animals per sex per dose:
- 6
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: daily (already one week before treatment, but the weekends excepted)
- Necropsy of survivors performed: no
Results and discussion
Effect levels
- Sex:
- male
- Dose descriptor:
- LC50
- Effect level:
- > 3.1 mg/L air (analytical)
- Remarks on result:
- other: no animals died within the 14 day observation period; exposure concentration of 3.1 mg/L corresponds to about 0.65 mg/L respirable dust (21% of dust particles had an aerodynamic diameter < 13 µm)
- Mortality:
- - no deaths occurred
- Clinical signs:
- other: - after exposure all animals had red stained fur
- Body weight:
- - immediately after exposure mild but transient weight loss but resumed a normal weight gain rate two days later
- Gross pathology:
- - not performed
Applicant's summary and conclusion
- Interpretation of results:
- not classified
- Remarks:
- Migrated information Criteria used for interpretation of results: other: CLP Regulation
- Conclusions:
- In an acute inhalation toxicity test male rats were exposed to dust concentrations up to 3.1 mg/L test item for 4 hours. Under the conditions tested no animal died during a 14 day observation period, resulting in a LC50 value of > 3.1 mg/L (corresponding to 0.651 mg/L respirable test item).
- Executive summary:
Acute inhalation toxicity of the test item has been investigated in male rats. They were exposed to 3.1 mg test substance per litre for 4 h. The test atmosphere contained only about 21% particles with an aerodynamic diameter (corresponding to 0.651 mg test item of respirable size). All animals survived the 14 day observation period, resulting in a LC50 value of > 3.1 mg/L (corresponding to 0.651 mg/L respirable test item) for the inhalation of dust.
Classification for acute inhalation toxicity is not necessary according to Regulation (EC) No 1272/2008.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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