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Diss Factsheets
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EC number: 941-876-2 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2012-03-09 - 2012-05-15
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- The Department of Health of the Government of the United Kingdom
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: All concentrations at 0 and 72 hr.
- Sample storage conditions before analysis: Stored at -20 degrees C until analysed. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Serial dilution of stock solution directly in culture medium
- Controls: Culture medium without test substance added
- Evidence of undissolved material (e.g. precipitate, surface film, etc): None noted - Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Strain: CCAP 278/4
- Source (laboratory, culture collection): Culture Collection of Algae and Protozoa, SAMS Research Services Ltd, Scottish Marine Institute, Oban, Argyll, Scotland.
- Method of cultivation: Periodic replenishment of culture medium
ACCLIMATION
- Acclimation period: None
- Culturing media and conditions (same as test or not): Same as test - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 24 +/- 1 degree C
- pH:
- Range 7.7-8.0
- Nominal and measured concentrations:
- Nominal concentrations: 0, 0.010, 0.032, 0.10, 0.32 and 1.0 mg/L.
Measured concentrations were 58-92%, with a concentration dependant decline. It was considered that the decline was caused by adsorbtion to the algal cells; therefore near nominal exposure was assumed. - Details on test conditions:
- TEST SYSTEM
- Test vessel: Flask
- Type (delete if not applicable): closed with polyurethane foam bungs
- Material, size, headspace, fill volume: glass, 250 mL, 100 mL test volume
- Aeration: orbital shaker only
- Control end cells density: 10^4-10^5 cells/mL
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: RO purified deionized water
- Culture medium different from test medium: No
- Intervals of water quality measurement:
OTHER TEST CONDITIONS
- Sterile test conditions: no
- Adjustment of pH: medium adjusted to 7.5 +/- 0.1 with 0.1N NaOH or HCl
- Photoperiod: Constant 24 hr
- Light intensity and quality: Warm white 7000 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : cell density, 72 hr
- Determination of cell concentrations: electronic particle counter
TEST CONCENTRATIONS
- Spacing factor for test concentrations: approximately 3
- Range finding study
- Test concentrations: 0.0010, 0.010, 0.10 and 1.0 mg/L
- Results used to determine the conditions for the definitive study: yes - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.16 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95 % CL- 0.14-0.19 mg/L
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.032 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 0.071 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: None noted
- Effect concentrations exceeding solubility of substance in test medium: None - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- EC50: 1.4 mg/L; 95 % CL 1.2-1.7 mg/L based on rate. 0.59 mg/L; 95% CL 0.53-0.65 mg/L based on yield.
- Other: Results within normal ranges - Reported statistics and error estimates:
- Statistical analysis was conducted via ANOVA, incorporating Bartlett's test for homogeneity, as well as Dunnett's multiple comparison test. (P>/= 0.05.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 72 hour EC50 of the test substance to P. subcapitata was found to be 0.16 mg/L (95% CL 0.14-0.19 mg/L) based on growth rate, and 0.084 mg/L (95% CL 0.077-0.092 mg/L) based on yield.
Reference
Description of key information
In this study, the acute toxicity of the test substance to Pseudokirchneriella was investigated in a 72-h static study (nominal concentrations between 0-1 mg/L). This results in a 72-h ErC50 of 0.16 mg/L and a 72 -h ErC10 of 0.071 mg/L.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 0.16 mg/L
- EC10 or NOEC for freshwater algae:
- 0.071 mg/L
Additional information
The algae growth inhibition study of Vrijenhoef (2012) was used as key study for PNEC derivation. The 72-h test was conducted according to OECD guideline 201. The nominal test concentrations were between 0 -1 mg/L. A 72-h ErC50 of 0.16 mg/L for Pseudokirchnerialla subcapitata was obtained in this study. The 72-h ErC10 was 0.071 mg/L. This indicates that the test substance is very toxic to aquatic algae.
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