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EC number: 242-768-1 | CAS number: 19035-79-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2013-01-08 to 2013-02-05
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- The study is regarded as reliable without restrictions because it was conducted in compliance with GLP regulation and guideline.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- Duplicate samples from the freshly prepared test medium of the only test concentration and the control were taken at the start of the test.
For the determination of the stability of the test item under the test conditions and of the maintenance of the test item concentration during the test period, duplicate samples from the test medium of the only test concentration and the control were collected at the end of the test (after 48 hours). - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
The test item was not well soluble in test water. To avoid physical effects of undissolved test item on the daphnids, no concentration above the solubility limit of the test item in test water was tested. Therefore, a supersaturated stock solution of nominal 100 mg test item/L was prepared by suspending 53.6 mg of test item in 536 mL test water. The stock solution was stirred for 23.75 hours in the dark to dissolve as much test item as possible. Then, the undissolved test item was separated by filtration (0.45 µm cellulose nitrate filter) and the filtrate was used as test medium. The test medium was prepared just before introduction of the daphnids (= start of the test). - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Source: The daphnids introduced in the test were taken from in house laboratory culture.
- Age at study initiation: From 2.5 to 18.5 hours old
- Method of breeding: The daphnids were bred under similar temperature and light conditions as used in the test. The cultivation of the parental daphnids was performed in the same reconstituted water as the test water used in the test. The test organisms were not first brood progeny. The daphnids in the stock culture were fed at least on all working days with green algae (Desmodesmus subspicatus).
- Feeding during test: none
ACCLIMATION
Was not necessary, since the test was performed in the same medium as the culturing. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Test temperature:
- 19 to 21 °C at test start
19 to 20 °C at test end - pH:
- 7.8 to 7.9 at test start
7.8 at test end - Dissolved oxygen:
- 8.8 to 9.1 mg/L at test start
8.3 to 8.5 mg/L at test end - Nominal and measured concentrations:
- A filtrate of a supersaturated stock suspension of nominal 100 mg/L. No measured concentrations are available (below LOD).
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 mL
- No. of organisms per vessel: 5
- No. of vessels per concentration: 4
- No. of vessels per control: 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Analytical grade salts and additives were added at the following nominal concentrations in deionised water (conductivity < 5 µS.cm-1):
Main Compounds
CaCl2x2 H2O 293.80 mg/L;
MgSO4x7 H2O 123.30 mg/L;
KCl 5.80 mg/L;
NaHCO3 64.80 mg/L;
Na2SiO3x9 H2O 10.00 mg/L;
NaNO3 0.27 mg/L;
KH2PO4 0.14 mg/L;
K2HPO4 0.18 mg/L
Trace Elements
H3BO3 2860 µg/L;
MnCl2x4 H2O 361 µg/L;
LiCl 306 µg/L;
RbCl 71 µg/L;
SrCl2x6 H2O 152 µg/L;
NaBr 16 µg/L;
Na2MoO4x2 H2O 63 µg/L;
CuCl2x2 H2O 17 µg/L;
ZnCl2 13 µg/L;
CoCl2x6 H2O 10 µg/L;
KI 3.3 µg/L;
Na2SeO3 2.2 µg/L;
NH4VO3 0.6 µg/L;
Na2EDTAx2 H2O 2.5 mg/L,
FeSO4x7 H2O 1.0 mg/L
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light : 8 h dark
- Light intensity: The light intensity was 690 - 750 lux (measured once during the test). - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: up to the water solubility of the test item no mortality of the test animals occurred.
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: up to the water solubility of the test item no mortality of the test animals occurred.
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: up to the water solubility of the test item no mortality of the test animals occurred.
- Duration:
- 24 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: up to the water solubility of the test item no mortality of the test animals occurred.
- Details on results:
- After 48 hours of exposure no immobilisation of the test animals was observed in the control and in the test group of nominal 100 mg/L.
- Results with reference substance (positive control):
- - Results with reference substance valid: yes (potassium dichromate)
- EC50: 0.706 mg/L
- NOEC: 0.28 mg/L - Validity criteria fulfilled:
- yes
- Conclusions:
- Up to the water solubility of the test item no mortality of the test animals occurred. Thus, the 48-h EC50 value based on mortality was determined to be greater than 100 mg/L and the 48-h NOEC was determined to be greater than 100 mg/L.
- Executive summary:
The purpose of this study was to evaluate the influence of the test item potassium hexadecyl hydrogen phosphate on the mobility of Daphnia magna according to OECD TG 202 and Regulation (EC) No 440/2008 method C.2. The test was performed as static limit test. Young daphnids were exposed 48 hours to test water containing the test item at the concentration of nominal 100 mg test item/L. This limit test was performed in compliance with the test guidelines to demonstrate that the test item has no toxic effect on daphnids up to at least this concentration.
This study was conducted using two treatment groups: one control and a filtrate of a supersaturated stock suspension of nominal 100 mg/L was tested (no concentrations above the solubility limit of the test item in the used test water were tested to avoid physical effects of undissolved test item onto the daphnids). Each treatment group contained 20 individuals. The mobility of the daphnids was determined in a static 48-hour test by visual observation after 24 and 48 hours. The collected samples analysed via LC-MS/MS-method. As the solubility of the test item in aquatic test media is very low, the concentrations of dissolved test item were below the Limit of Detection (0.2 µg test item/L). However, all reported results refer to nominal loading rate. The EC50 for the reference substance potassium dichromate was determined to be 0.706 mg/L. All validation criteria according to OECD TG 202 were met. The 48-hour NOEC was determined to be 100 mg test item/L (nominal). The 48-hour EC50 value was determined to be greater than 100 mg test item/L (nominal). Thus, no toxic effects on daphnia magna were observed up to the water solubility of the test item.
Reference
Table 1 Influence of the test item on the Mobility of Daphnia magna
Nominal Concentration |
No. of daphnids tested |
No. of immobilised daphnids after |
% of immobilised daphnids after |
||
[mg test item/L] |
|
24 h |
48 h |
24 h |
48 h |
Control |
20 |
0 |
0 |
0 |
0 |
100 |
20 |
0 |
0 |
0 |
0 |
Table 2 Biological endpoints after 48 hours exposure period
EC50 [mg test item/L]: |
>100 |
95 % CI [mg test item/L]: |
n.d. |
EC20 [mg test item/L]: |
>100 |
95 % CI [mg test item/L]: |
n.d. |
EC10 [mg test item/L]: |
>100 |
95 % CI [mg test item/L]: |
n.d. |
NOEC [mg test item/L]: |
100 |
LOEC [mg test item/L]: |
>100 |
Values refer to nominal test concentrations
CI: Confidence interval
n.d.: not determinable
All Endpoints were determined directly from the raw data.
Table 3 Results for the Determination of the Test Item in the Test Samples
sample description |
age |
test item found [µg/L]* |
D.F. |
calculated test item [µg/L]* |
|
day |
[h] |
||||
control |
0 |
0 |
<LOD |
100 |
n.a. |
control |
2 |
48 |
<LOD |
100 |
n.a. |
Filtrate of 100 mg test item/L |
0 |
0 |
<LOD |
100 |
n.a. |
Filtrate of 100 mg test item/L |
0 |
0 |
<LOD |
100 |
n.a. |
Filtrate of 100 mg test item/L |
2 |
48 |
<LOD |
100 |
n.a. |
Filtrate of 100 mg test item/L |
2 |
48 |
<LOD |
100 |
n.a. |
* The tabulated results represent rounded results calculated on the exact raw data
** The results represent rounded values
LOD: Limit
of Detection = 1.6
µg test item/L
n.a.: not
applicable
D.F.: Dilution factor
Description of key information
Up to the water solubility of the test item no mortality of the test animals occurred. Thus, the 48-h EC50 value based on mortality was determined to be greater than 100 mg/L.
Key value for chemical safety assessment
Additional information
The purpose of this study was to evaluate the influence of the test item potassium hexadecyl hydrogen phosphate on the mobility of Daphnia magna according to OECD TG 202 and Regulation (EC) No 440/2008 method C.2. The test was performed as static limit test. Young daphnids were exposed 48 hours to test water containing the test item at the concentration of nominal 100 mg test item/L. This limit test was performed in compliance with the test guidelines to demonstrate that the test item has no toxic effect on daphnids up to at least this concentration.
This study was conducted using two treatment groups: one control and a filtrate of a supersaturated stock suspension of nominal 100 mg/L was tested (no concentrations above the solubility limit of the test item in the used test water were tested to avoid physical effects of undissolved test item onto the daphnids). Each treatment group contained 20 individuals. The mobility of the daphnids was determined in a static 48-hour test by visual observation after 24 and 48 hours. The collected samples analysed via LC-MS/MS-method. As the solubility of the test item in aquatic test media is very low, the concentrations of dissolved test item were below the Limit of Detection (0.2 µg test item/L). However, all reported results refer to nominal loading rate. The EC50 for the reference substance potassium dichromate was determined to be 0.706 mg/L. All validation criteria according to OECD TG 202 were met. The 48-hour NOEC was determined to be 100 mg test item/L (nominal). The 48-hour EC50 value was determined to be greater than 100 mg test item/L (nominal). Thus, no toxic effects on daphnia magna were observed up to the water solubility of the test item.
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