2-methylbutan-2-ol

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1968-09-20 to 1968-09-24

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study predating guidelines and GLP, but meeting the principles of OECD TG 401.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1968

Report date:

1968

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Deviations:

not applicable

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

2-methylbutan-2-ol

EC Number:

200-908-9

EC Name:

2-methylbutan-2-ol

Cas Number:

75-85-4

Molecular formula:

C5H12O

IUPAC Name:

2-methylbutan-2-ol

Details on test material:

- Name of test material: tertiary amyl alcohol

Test animals

Species:

rat

Strain:

other: "US-rats"

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 142 - 240 g (males); 120 - 174 g (females)

ENVIRONMENTAL CONDITIONS
- no data

IN-LIFE DATES: From: 1968-09-20 To: 1968-10-07

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

other: aqueous tragacanth emulsion

Details on oral exposure:

VEHICLE
- Vehicle concentration: 30% (6400, 3200 mm³/kg bw) , 20% (1600 mm³/kg bw), 2% (200 mm³/kg bw)

Doses:

6400, 3200, 1600 or 200 mm³/kg bw

No. of animals per sex per dose:

10 males and 10 females

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: body weight taken at study start only for dose determination, but not reported
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs monitored but frequency unknown, gross necropsy on fatalities

Statistics:

not performed

Results and discussion

Effect levels

Effect levels 1

Sex:

male/female

Dose descriptor:

LD50

Effect level:

6 400 other: mm³/kg bw

Based on:

test mat.

Remarks on result:

other: 5184 mg/kg bw

Effect levels 2

Sex:

male

Dose descriptor:

LD50

Effect level:

> 6 400 other: mm³/kg bw

Based on:

test mat.

Remarks on result:

other: > 5184 mg/kg bw

Effect levels 3

Sex:

female

Dose descriptor:

LD50

Effect level:

ca. 5 000 other: mm³/kg bw

Based on:

test mat.

Remarks on result:

other: 4050 mg/kg bw

Mortality:

Totals after 14-days:
6400 mm³/kg bw: 10/20
3200 mm³/kg bw: 1/20
1600 mm³/kg bw: 0/20
200 mm³/kg bw: 0/20

Males:
6400 mm³/kg bw: 2/10 (1/10 within 1 h, 2/10 within 24 h)
3200 mm³/kg bw: 1/10 (0/10 within 1 h, 1/10 within 24 h)
1600 mm³/kg bw: 0/10
200 mm³/kg bw: 0/10

Females:
6400 mm³/kg bw: 8/10 (7/10 within 1 h, 8/10 within 24 h)
3200 mm³/kg bw: 0/10
1600 mm³/kg bw: 0/10
200 mm³/kg bw: 0/10

Clinical signs:

other: 3200 and 6400 mm³/kg bw Immediately after application: prone or lateral position, irregular respiration, narcosis; 1 day: panting, ruffled fur; 2 days: fully recovered 1600 mm³/kg bw Immediately after application: dyspnoea, watery secretion from snout, wa

Gross pathology:

Organs: no abnormalities detected
Perished animals: sepsis

Other findings:

none