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EC number: 271-678-5 | CAS number: 68603-87-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with national standard methods with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
- Version / remarks:
- , Draft 1984
- GLP compliance:
- not specified
- Analytical monitoring:
- no
- Test organisms (species):
- activated sludge, industrial
- Details on inoculum:
- Trockensubstanz: 5g/L
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 30 min
- Test temperature:
- room temperature: 20-25°C
- Nominal and measured concentrations:
- nominal
- Duration:
- 30 min
- Dose descriptor:
- other: EC20
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Conclusions:
- An EC20 of >100 mg/L was observed.
- Executive summary:
A test with activated sludge with a duration of 0.5 hours was performed according to the ISO method 8192. An EC20 of >100 mg/L was observed.
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- other: test material was adipic acid residue
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- no
- Analytical monitoring:
- no
- Test organisms (species):
- activated sludge
- Details on inoculum:
- - Initial biomass concentration: 6.0 g/L (dry weight)
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- pH:
- 7.3 - 8.0
- Nominal and measured concentrations:
- nominal concentrations: 1000, 1800, 3200, 5600 and 10000 mg/l
- Details on test conditions:
- Type: aquatic
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 7 910 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Conclusions:
- An EC50 of 7910 mg/L was observed.
- Executive summary:
A test with activated sludge with a duration of 3 hours was performed according to the OECD TG 209. An EC50 of 7910 mg/L was observed.
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Justification for type of information:
- Read-Across from glutaric acid, which is a structural analogue and serves well as a representative substance for the carboxylic acid mixture.
- Principles of method if other than guideline:
- Growth Impairment Test
Test was performed according to the method described by Schultz TW (1997) TETRATOX: Tetrahymena pyriformis population growth impairment endpoint. A surrogate for fish lethality. Toxicol. Methods 7, 289-309 - GLP compliance:
- not specified
- Analytical monitoring:
- no
- Vehicle:
- yes
- Details on test solutions:
- The solvent DMSO has low toxicity to tetrahymena, low volatility, and high ability to dissolve most organic chemicals. (Concentration : 0.75 % DMSO) This concentration shows no effect on Tetrahymena growth.
- Test organisms (species):
- Tetrahymena pyriformis
- Details on inoculum:
- pH 5.0 - 8.6 (optimum: 7.35)
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 40 h
- Test temperature:
- 27+/- 1°C
- pH:
- 7.4 (Control cultures: pH 5.5)
- Nominal and measured concentrations:
- A minimum of 5 different concentration with duplicate flasks of each concentration.
- Details on test conditions:
- - 250 ml Erlenmeyer flask (foam-stoppered)
- containing 50 mL sterile, semidefined proteose-peptone-based medium
- inoculated with an initial density: 1000 - 5000 cells/mL, currently ca. 2500 cells/mL
- duplicate controls
- blank - Duration:
- 40 h
- Dose descriptor:
- other: inhibitory growth concentration IGC50
- Effect conc.:
- 591.02 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks on result:
- other: Adipic acid
- Duration:
- 40 h
- Dose descriptor:
- other: inhibitory growth concentration IGC50
- Effect conc.:
- 574.98 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks on result:
- other: Glutaric acid
- Duration:
- 40 h
- Dose descriptor:
- other: inhibitory growth concentration IGC50
- Effect conc.:
- 1 027.32 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks on result:
- other: Succinic acid
- Details on results:
- The IGC50 is calculated by probit analysis.
- percent control-normalized absorbance used as dependent variable
- toxicant concentration in mg/L used as the independent variable
- recording of slope and intersept of probit regression equation, Chi-squared value
- normaly Chi-squared value > 0.9 - Validity criteria fulfilled:
- yes
- Conclusions:
- Tests on growth inhibition with Tetrahymena pyriformis (Schultz, 1997) show
the following inhibitory growth concentrations (IGC50) after 40 hours:
Adipic acid: IGC50 = 591.02 mg/L
Glutaric acid: IGC50 = 574.98 mg/L
Succinic acid: IGC50 = 1027.32 mg/L - Executive summary:
Tests on growth inhibition with Tetrahymena pyriformis (Schultz, 1997) show
the following inhibitory growth concentrations (IGC50) after 40 hours:
Adipic acid: IGC50 = 591.02 mg/L
Glutaric acid: IGC50 = 574.98 mg/L
Succinic acid: IGC50 = 1027.32 mg/L
Referenceopen allclose all
- population density is measured spectrophotometrically
- The 50 % inhibitory growth concentration in mg/L (IGC50) and the 95% fiducial interval are determined for each test compound.
Description of key information
An IC50 of 574.98 mg/L was determined for glutaric acid, one of the constituent of the dicarboxylic acids, in a growth inhibition test with Tetrahymena pyriformis (Schultz, 1997).
Key value for chemical safety assessment
- EC50 for microorganisms:
- 574.98 mg/L
Additional information
Concerning the toxicity to microorganisms, there are tests with mixed inoculum and single species avaialbale using the dicarboxylic acids mixture as test substance as well as the single acids.
A test with activated sludge with a duration of 3 hours using a residue from adipic acid manufacturing is available. The test substance contains the following composition: 60 % adipic acid, 14 % glutaric acid, 6 % succinic acid, 10 % carbon, 6.5 % vanadium pentoxide, 3 % copper nitrate and 5 % copper (Bayer, 1988b). The result of EC50 = 7911 mg/l, shows that the compounds other than the dicarboxylic acids did not highly affect the microorganisms.
Another test with activated sludge with a duration of 0.5 hours was performed according to the ISO method 8192 using a dicarboxylic acids mixture without further specification as test substance. An EC20 of >100 mg/L was observed (BASF, 1988).
In a 17 hours test with the dicarboxylic acid mixture towards Pseudomonas putida according to the German standard method DIN-38412 Part 8 (Cell Multiplication Inhibition Test), an EC50 of 91 mg/l was observed (BASF AG 1990). But in this test the pH values in the test solutions ranged from 4.7 (125 mg/l) to 5.5 (3.91 mg/l). As at higher concentrations tested the pH was always <5, it cannot be excluded that the toxicity observed was due to pH effects. Therefore, the study is stated to be reliable, but should not be used for the hazard assessment.
Tests on growth impairment with Tetrahymena pyriformis (Schultz, 1997) using the single acids, adipic-, succinic- and glturaic acid as test substance, results in the following growth inhibitory
concentrations: an IC50 of 591.02 mg/L after 40 hours was found for adipic acid, for glutaric acid an IC50 of 574.98 mg/L was observed and an IC50 of 1027.32 mg/L was determined for succinic acid.Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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