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EC number: 212-222-7 | CAS number: 770-35-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to reproduction
Administrative data
- Endpoint:
- two-generation reproductive toxicity
- Remarks:
- based on test type (migrated information)
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2000
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP-study according to OECD guideline 416.
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 000
- Report date:
- 2000
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 416 (Two-Generation Reproduction Toxicity Study)
- Principles of method if other than guideline:
- not applicable
- GLP compliance:
- yes
- Limit test:
- no
Test material
- Reference substance name:
- 1-phenoxypropan-2-ol
- EC Number:
- 212-222-7
- EC Name:
- 1-phenoxypropan-2-ol
- Cas Number:
- 770-35-4
- Molecular formula:
- C9H12O2
- IUPAC Name:
- 1-phenoxypropan-2-ol
- Reference substance name:
- 1-phenylpropan-2-ol
- EC Number:
- 211-821-0
- EC Name:
- 1-phenylpropan-2-ol
- Cas Number:
- 698-87-3
- IUPAC Name:
- 1-phenylpropan-2-ol
- Details on test material:
- Identity: Protectol PP
Synonyms: Propylene Glycol Phenyl Ether, PPh
Purity: Isomeric mixture (85/15). Presumably, this means 85% 1-phenoxy-2-propanol (secondary alcohol, CAS No. 770-35-4) and 15% 2-phenoxy-1-propanol (primary alcohol, CAS No. 41593-38-8).
The stability of the test material in drinking water was established over a period of 96 hrs at room temperature and dose concentrations.
Constituent 1
Constituent 2
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: not specified
- Age at study initiation: (P) x approximately 6 weeks; (F1) (continuous exposure)
- Weight at study initiation: (P) Males: 102 - 140 g; Females: 90 - 122g; (F1) Males: x-x g; Females: x-x g
- Fasting period before study: none
- Housing: individual
- Diet (e.g. ad libitum): not specified
- Water (e.g. ad libitum): not specified
- Acclimation period: not specified
ENVIRONMENTAL CONDITIONS
- Temperature (°C): standard conditions
- Humidity (%): standard conditions
- Air changes (per hr): standard conditions
- Photoperiod (hrs dark / hrs light): standard conditions
Administration / exposure
- Route of administration:
- oral: drinking water
- Type of inhalation exposure (if applicable):
- other: not applicable
- Vehicle:
- water
- Details on exposure:
- PREPARATION OF DOSING SOLUTIONS: Propylene glycol phenyl ether was continuously administered to male & female F0 and F1 animals with drinking water at concentrations of 0, 100, 1000, 5000 ppm, respectively, until animals were terminated. Solutions were prepared once or twice a week. Propylene glycol phenyl ether concentrations were checked at start and at 3-monthly intervals during the administration period, and at its end
- Details on mating procedure:
- - M/F ratio per cage: 1:1 (male:female)
- Length of cohabitation: 3 weeks
- Proof of pregnancy: [sperm in vaginal smear] referred to as [day 0] of pregnancy
- For those pairs failing to mate, replacement of first male by another male with proven fertility was not done
- Further matings after two unsuccessful attempts: [no]
- After successful mating each pregnant female was caged (how): individual
- Any other deviations from standard protocol: not specified - Analytical verification of doses or concentrations:
- yes
- Details on analytical verification of doses or concentrations:
- Propylene glycol phenyl ether concentrations were checked at start and at 3-monthly intervals during the administration period, and at its end. The stability of the test material in drinking water was established over a period of 96 hrs at room temperature and dose concentrations.
- Duration of treatment / exposure:
- Exposure period: 26 weeks
Premating exposure period (males): 77 days
Premating exposure period (females): 77 days
Duration of test: 40 weeks - Frequency of treatment:
- 7 days/week
- Details on study schedule:
- - F1 parental animals not mated until [approximately 10-13] weeks after selected from the F1 litters.
- Selection of parents from F1 generation when pups were [21] days of age.
- Age at mating of the mated animals in the study: [10-13] weeks
Doses / concentrationsopen allclose all
- Remarks:
- Doses / Concentrations:
0 ppm
Basis:
nominal in water
- Remarks:
- Doses / Concentrations:
100 ppm (nominal in water)
Basis:
other: 11.3 mg/kg body weight/day (mean dose)
- Remarks:
- Doses / Concentrations:
1000 ppm (nominal in water)
Basis:
other: 113.9 mg/kg body weight/day (mean dose)
- Remarks:
- Doses / Concentrations:
5000 ppm (nominal in water)
Basis:
other: 477.5 mg/kg body weight/day (mean dose)
- No. of animals per sex per dose:
- 25 rats/sex/dose
- Control animals:
- yes
- Details on study design:
- - Dose selection rationale: not specified
- Rationale for animal assignment: random - Positive control:
- not applicable
Examinations
- Parental animals: Observations and examinations:
- CAGE SIDE OBSERVATIONS: Yes
- Time schedule: daily
DETAILED CLINICAL OBSERVATIONS: No data
BODY WEIGHT: Yes
- Time schedule for examinations: Body weights of parental animals were recorded weekly with the following exceptions for females:
-during mating periods females were weighed on day 0 and on days 7, 14, 20 post coitum
-females without evidence of sperm were not weighed during the mating period
-females with litters were weighed on days 1,4,7,14 and 21 post partum
-females without litters were not weighed during lactation period
-F0 and F1 females were weighed after weaning of the last F1 or F2 pups, parallel to the F0 and F1 males, once weekly until termination
FOOD CONSUMPTION: Yes
- Time schedule for examinations: Food consumption was determined once a week during the premating phase of both F0 and F1, and weekly during
gestation. During lactation it was determined weekly on days 1, 4, 7 and 14, but not during 14-21 as required by the guideline since the pups start to consume considerable amounts of solid food. Food consumption was not determined for F0 and F1 males after the premating phase and for females without evidence of sperm or without litters in the lactation phase
WATER CONSUMPTION AND COMPOUND INTAKE (if drinking water study): Yes
- Time schedule for examinations: Water consumption of F0 and F1 parental animals was determined once a week during the premating periods. After premating (10th week), water consumption of females during gestation was determined for days 0-1, 6-7, 13-14 and 19-20 post coitum, and during lactation period for days 1-2, 4-5, 7-8 and 14-15 pp (post parturition). After day 15 pp water consumption of the F0 and F1 dams was not determined since from then onwards the pups begin to consume considerable amounts of water. Water consumption was not determined for F0 and F1 males after the premating phase and for females without evidence of sperm or without litters in the lactation phase. Intake of PPh was calculated from the daily water consumption.
- Oestrous cyclicity (parental animals):
- Estrous cycle length and normality were evaluated for all F0 and F1 females for a minimum of 3 weeks prior to mating; this was continued throughout the mating period. Vaginal smear was examined at necropsy to determine the stage of the estrous cycle for each F0 and F1 female rats
- Sperm parameters (parental animals):
- Parameters examined in [all] male parental generations: Sperm parameters were determined (sperm motility, morphology, sperm head count in testis and in cauda epididymis) immediately after necropsy and weighing the right testis and cauda epididymis. Sperm motility examinations were randomized; sperm morphology and sperm head count were evaluated in control and highest dose animals only
- Litter observations:
- STANDARDISATION OF LITTERS
- Performed on day 4 postpartum: [yes]
- If yes, maximum of [8] pups/litter ([4]/sex/litter as nearly as possible); excess pups were killed and discarded. Also, litters with less than 8 pups were not standardized
PARAMETERS EXAMINED
The following parameters were examined in [F1 / F2 / F3] offspring: [number and sex of pups, stillbirths, live births, postnatal mortality, presence of gross anomalies, weight gain, physical or behavioural abnormalities]
GROSS EXAMINATION OF DEAD PUPS:
[yes, for external and internal abnormalities; possible cause of death was determined for pups born or found dead.] - Postmortem examinations (parental animals):
- SACRIFICE
- Male animals: All surviving animals [as soon as possible after the last litters in each generation were produced.]
- Maternal animals: All surviving animals [after the last litter of each generation was weaned.]
GROSS NECROPSY
- Gross necropsy consisted of [external and internal examinations including the cervical, thoracic, and abdominal viscera.] Organ weights of all F0 and F1 parental animals terminated at schedule were determined: body weight, liver, kidneys, adrenals, testes, epididymides (total, cauda), prostate
gland, seminal vesicles with coagulation glands, ovaries, uterus (with cervix and oviducts), thymus, spleen, brain, pituitary gland
HISTOPATHOLOGY / ORGAN WEIGHTS
- The following organs were fixed or embedded for histopathology: vagina, cervix uteri, uterus, ovaries, oviducts, left testicle and epididymides, seminal vesicles, coagulating glands, prostate gland, pituitary gland, liver, kidneys, urinary bladder, thymus, spleen, brain, adrenal glands, and all gross lesions. In ovaries, a Differential Ovarian Follicle Count (DOFC) was also included - Postmortem examinations (offspring):
- SACRIFICE
- The F1 offspring not selected as parental animals and all F2 offspring were sacrificed at [21] days of age.
- These animals were subjected to postmortem examinations (macroscopic examination) as follows: all pups with scheduled termination, i.e. those culled on day 4 pp, and those terminated at day 21, were examined externally and eviscerated; organs were assessed macroscopically, and additionally, if this was deemed necessary due to notable findings and abnormalities. The same procedure was applied to all stillborn pups and all pups that died up to weaning. After scheduled termination, of the pups organs brain, spleen and thymus were weighed of one pup/sex and litter from F1 and F2 pups.
GROSS NECROPSY
- Gross necropsy consisted of [external and internal examinations including the cervical, thoracic, and abdominal viscera.]
HISTOPATHOLOGY / ORGAN WEIGTHS
- not specified - Statistics:
- Extensive statistical evaluation of the clinical data included the use of the Dunnett-test for comparison with the control group, Fisher's exact test, Wilcoxon test and Kruskal-Wallis-test. Statistical evaluation of the organ weight parameters involved Kruskal-Wallis test and Wilcoxon test, if p was equal to or < 0.05. Follicle data from DOFC were evaluated using a Wilcoxon test
- Reproductive indices:
- Male reproduction indices (mating and fertility index) were calculated. For females, indices pertaining to mating, fertility, gestation were calculated.
- Offspring viability indices:
- For F1 and F2 litters, live birth index was calculated (percentage of liveborn pups). Postimplantation loss was calculated after termination of females from the number of implantations and pups delivered
Results and discussion
Results: P0 (first parental generation)
General toxicity (P0)
- Clinical signs:
- no effects observed
- Body weight and weight changes:
- effects observed, treatment-related
- Food consumption and compound intake (if feeding study):
- effects observed, treatment-related
- Organ weight findings including organ / body weight ratios:
- no effects observed
- Histopathological findings: non-neoplastic:
- no effects observed
- Other effects:
- no effects observed
Reproductive function / performance (P0)
- Reproductive function: oestrous cycle:
- no effects observed
- Reproductive function: sperm measures:
- no effects observed
- Reproductive performance:
- no effects observed
Details on results (P0)
BODY WEIGHT AND FOOD CONSUMPTION (PARENTAL ANIMALS) - F0 animals - In the 5000 ppm group, compared with controls significant reductions in consumption of water during premating (-20% males, -22% females), gestation (-21%) and lactation (-19%) and in food consumption (significant on some days) during premating (-6% males, -5% females), gestation (-5%) and lactation (-8%). This was paralleled by a clearly decreased body weight (bw) and body weight change (bwc) in males (-10% each). In females, reduced values were also seen during premating, gestation and lactation (bw: -6, -7, -11%; bwc: -8, -14, -8%)
F1 animals - Significantly decreased water consumption during premating (up to -20% males, -29% females), gestation (-25%) and lactation (-33%). Mainly significantly reduced food consumption during premating (-8% males and females), gestation (-12%) and lactation (-18%). Clearly decreased bw and bwc in males (bw -19%, bwc ca. -10%). In females, reduced values were seen during premating, gestation and lactation (bw: -22, -15, -16%; bwc: +/-0, -25, -31%). Though clear effects on body weight were seen in both, males and females, level of significance was achieved only for single periods
TEST SUBSTANCE INTAKE (PARENTAL ANIMALS) - F0 animals - In the 5000 ppm group, compared with controls significant reductions in consumption of water during premating (-20% males, -22% females), gestation (-21%) and lactation (-19%) and in food consumption (significant on some days) during premating (-6% males, -5% females), gestation (-5%) and lactation (-8%). This was paralleled by a clearly decreased body weight (bw) and body weight change (bwc) in males (-10% each). In females, reduced values were also seen during premating, gestation and lactation (bw: -6, -7, -11%; bwc: -8, -14, -8%)
F1 animals - Significantly decreased water consumption during premating (up to -20% males, -29% females), gestation (-25%) and lactation (-33%). Mainly significantly reduced food consumption during premating (-8% males and females), gestation (-12%) and lactation (-18%). Clearly decreased bw and bwc in males (bw -19%, bwc ca. -10%). In females, reduced values were seen during premating, gestation and lactation (bw: -22, -15, -16%; bwc: +/-0, -25, -31%). Though clear effects on body weight were seen in both, males and females, level of significance was achieved only for single periods
REPRODUCTIVE FUNCTION: ESTROUS CYCLE (PARENTAL ANIMALS) - No substance-related adverse effects seen with respect to reproductive function
REPRODUCTIVE FUNCTION: SPERM MEASURES (PARENTAL ANIMALS) - No substance-related adverse effects seen with respect to reproductive function
REPRODUCTIVE PERFORMANCE (PARENTAL ANIMALS) - No substance-related adverse effects seen with respect to reproductive performance
ORGAN WEIGHTS (PARENTAL ANIMALS) -No substance-related adverse effects seen with respect to organ weights
GROSS PATHOLOGY (PARENTAL ANIMALS) -No substance-related adverse effects seen with respect to pathology
HISTOPATHOLOGY (PARENTAL ANIMALS) - No substance-related adverse effects seen with respect to histopathology
OTHER FINDINGS - Propylene glycol phenyl ether intake by premating F0 and F1 females was slightly enhanced compared with values for males (total treatment period) in all dose groups. PPh intake was markedly enhanced during gestation and lactation when compared with premating animals (up to ca. 1.5 fold in F0 females). Intake of PPh was also enhanced in F1 parental animals compared with F0 parents
Effect levels (P0)
open allclose all
- Dose descriptor:
- NOAEL
- Effect level:
- 5 000 ppm
- Based on:
- test mat.
- Sex:
- male/female
- Basis for effect level:
- other: based on reproductive performance and fertility
- Dose descriptor:
- NOAEL
- Effect level:
- 1 000 ppm
- Based on:
- test mat.
- Sex:
- male/female
- Basis for effect level:
- other: see 'Remark'
Results: F1 generation
General toxicity (F1)
- Clinical signs:
- no effects observed
- Mortality / viability:
- no mortality observed
- Body weight and weight changes:
- effects observed, treatment-related
- Sexual maturation:
- effects observed, treatment-related
- Organ weight findings including organ / body weight ratios:
- effects observed, treatment-related
- Gross pathological findings:
- no effects observed
- Histopathological findings:
- no effects observed
Details on results (F1)
CLINICAL SIGNS (OFFSPRING) - No substance-related adverse effects seen with respect to clinical examination, sexual maturation (F1 pups only), pup organ weights, pathology
BODY WEIGHT (OFFSPRING) - F1 pups - Significantly lower bw at weaning on d 21 (-11%, both sexes combined) and lower bwc (-13%). Concomitantly a retardation in sexual maturation as evidenced by delayed preputial separation and vaginal opening in the selected F1 male and female animals
F2 pups - Significantly lower mean bw in male pups from day 4 pp (post parturition) onwards. During d 7-21 pp lowered by 23% (both sexes combined). Significantly impaired body weight change in male and female pups (-26%; d 4-21 pp)
SEXUAL MATURATION (OFFSPRING) - F1 pups - Significantly lower bw at weaning on d 21 (-11%, both sexes combined) and lower bwc (-13%). Concomitantly a retardation in sexual maturation as evidenced by delayed preputial separation and vaginal opening in the selected F1 male and female animals
ORGAN WEIGHTS (OFFSPRING) - F2 pups - During pathological examinations, organ weight changes (both sexes combined) were seen as follows: significantly lower mean absolute weights of brain (-6%), thymus (-23%) and spleen (-34%) compared to controls. Significant relative organ weight changes were noted for brain (+30%) and spleen (-18%). The relationship to treatment of reduced relative spleen weights to body weights in the progeny of high dose animals was unclear, since a similar effect was not noted in the parental animals
GROSS PATHOLOGY (OFFSPRING) - No substance-related adverse effects seen with respect to clinical examination, sexual maturation (F1 pups only), pup organ weights, pathology
OTHER FINDINGS - Propylene glycol phenyl ether intake by premating F0 and F1 females was slightly enhanced compared with values for males (total treatment period) in all dose groups. PPh intake was markedly enhanced during gestation and lactation when compared with premating animals (up to ca. 1.5 fold in F0 females). Intake of PPh was also enhanced in F1 parental animals compared with F0 parents
Effect levels (F1)
- Dose descriptor:
- NOAEL
- Generation:
- F1
- Effect level:
- 1 000 ppm
- Based on:
- test mat.
- Sex:
- male/female
- Basis for effect level:
- other: see 'Remark'
Results: F2 generation
Effect levels (F2)
- Dose descriptor:
- NOAEL
- Generation:
- F2
- Effect level:
- 1 000 ppm
- Based on:
- test mat.
- Sex:
- male/female
- Basis for effect level:
- other: see 'Remark'
Overall reproductive toxicity
- Reproductive effects observed:
- not specified
Any other information on results incl. tables
none
Applicant's summary and conclusion
- Conclusions:
- Propylene glycol phenyl ether was continuously administered with drinking water to
rats over two parental generations at concentrations of 0,
100, 1000 and 5000 ppm.
Under the conditions of this study, NOAELs were established as follows: NOAEL for reproductive performance and fertility: 5000 ppm (about 477.5 mg PPh/kg body weight/day) for the F0 and F1 parents; NOAEL for developmental toxicity: 1000 ppm (about 113.9 mg PPh/kg body weight/day) for the F1 and F2 progeny; NOAEL for general systemic toxicity: 1000 ppm (about 113.9 mg PPh/kg body weight/day) for the F0 and F1 parents. Thus, developmental toxicity was seen only at a dose which was also toxic to the parent animals. No sign of teratogenicity was seen at either dose in this study.
Reproductive performance or fertility was not affected in F0 or F1 parental animals of either dose group. Estrous cycle, mating behavior, conception, gestation, parturition, lactation and weaning as well as sperm parameters, sexual organ weights, and gross and histopathological findings of these organs were similar between control and treated animals.
Signs of general, systemic toxicity were noted in both parental generations (F0 and F1) in groups receiving 5000 ppm, but not in others. Toxicity was characterized by decreased water and food consumption, decreased body weight and body weight gain in parental F0 an F1 males and females. Pathology and histopathology did not reveal substance-related adverse effects in F0 and F1 parental animals.
The clinical, gross and histopathological examinations in F0 and F1 parental animals from the low and intermediate dose groups did not yield any indication of systemic toxicity.
Substance-related signs of developmental toxicity were seen in progeny of the high dose (5000 ppm) F0 and F1 parents in terms of reduced pup body weight and body weight gain. This is directly related to lower absolute weights of the thymus, spleen and brain in pups and delayed sexual maturation. Moreover, reproduction parameters of these animals were not adversely affected after gaining sexual maturity. This supports the view that delayed preputial separation and vaginal opening resulted from a general retardation of physical development. No signs of developmental toxicity were seen in pups from groups receiving medium or low doses (1000 or 100 ppm, respectively.).
Under the conditions of this study, NOAELs were established as follows:
NOAEL for reproductive performance and fertility: 5000 ppm (about 477.5 mg PPh/kg body weight/day) for the F0 and F1 parents
NOAEL for developmental toxicity: 1000 ppm (about 113.9 mg PPh/kg body weight/day) for the F1 and F2 progeny
NOAEL for general systemic toxicity: 1000 ppm (about 113.9 mg PPh/kg body weight/day) for the F0 and F1 parents
Thus, developmental toxicity was seen only at a dose which was also toxic to the parent animals. No sign of teratogenicity was seen at either dose in this study. - Executive summary:
In this study, Propylene glycol phenyl ether was continuously administered with drinking water to rats over two parental generations at concentrations of 0, 100, 1000 and 5000 ppm.
Reproductive performance or fertility was not affected in F0 or F1 parental animals of either dose group. Estrous cycle, mating behavior, conception, gestation, parturition, lactation and weaning as well as sperm parameters, sexual organ weights, and gross and histopathological findings of these organs were similar between control and treated animals.
Signs of general, systemic toxicity were noted in both parental generations (F0 and F1) in groups receiving 5000 ppm, but not in others. Toxicity was characterized by decreased water and food consumption, decreased body weight and body weight gain in parental F0 an F1 males and females. Pathology and histopathology did not reveal substance-related adverse effects in F0 and F1 parental animals.
The clinical, gross and histopathological examinations in F0 and F1 parental animals from the low and intermediate dose groups did not yield any indication of systemic toxicity.
Substance-related signs of developmental toxicity were seen in progeny of the high dose (5000 ppm) F0 and F1 parents in terms of reduced pup body weight and body weight gain. This is directly related to lower absolute weights of the thymus, spleen and brain in pups and delayed sexual maturation. Moreover, reproduction parameters of these animals were not adversely affected after gaining sexual maturity. This supports the view that delayed preputial separation and vaginal opening resulted from a general retardation of physical development. No signs of developmental toxicity were seen in pups from groups receiving medium or low doses (1000 or 100 ppm, respectively.).
Under the conditions of this study, NOAELs were established as follows:
NOAEL for reproductive performance and fertility: 5000 ppm (about 477.5 mg PPh/kg body weight/day) for the F0 and F1 parents
NOAEL for developmental toxicity: 1000 ppm (about 113.9 mg PPh/kg body weight/day) for the F1 and F2 progeny
NOAEL for general systemic toxicity: 1000 ppm (about 113.9 mg PPh/kg body weight/day) for the F0 and F1 parents
Thus, developmental toxicity was seen only at a dose which was also toxic to the parent animals. No sign of teratogenicity was seen at either dose in this study.
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