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EC number: 700-610-3 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2008/06/10
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- The reliabitity is rated 2 because the study followed a standard guideline of reference (NF EN ISO 6341), which describes a procedure designed to evaluate this endpoint. The results were reviewed and assessed as valid. However the study was not conducted under GLP conditions.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 008
- Report date:
- 2008
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))
- GLP compliance:
- no
Test material
- Reference substance name:
- Bis{tris[4-(mono and dimethylamino)phenyl]methylium} 2-(bis{4-[ethyl(3- sulfonatobenzyl)amino]phenyl}methyliumyl)benzenesulfonate
- IUPAC Name:
- Bis{tris[4-(mono and dimethylamino)phenyl]methylium} 2-(bis{4-[ethyl(3- sulfonatobenzyl)amino]phenyl}methyliumyl)benzenesulfonate
- Reference substance name:
- Bis{tris[4-(mono and dimethylamino)phenyl]methylium} 2-(bis{4-[ethyl(3-sulfonatobenzyl)amino]phenyl}methyliumyl)benzenesulfonate
- EC Number:
- 700-610-3
- Molecular formula:
- UVCB substance - not applicable
- IUPAC Name:
- Bis{tris[4-(mono and dimethylamino)phenyl]methylium} 2-(bis{4-[ethyl(3-sulfonatobenzyl)amino]phenyl}methyliumyl)benzenesulfonate
- Details on test material:
- - Name of test material (as cited in study report): Sepisol Fast Blue 2BR
- Substance type:organic salt
- Physical state and appearance: dark blue powder
- Lot/batch No.: 905536
- Stability under test conditions: stable
- Storage condition of test material: at ambient temperature
Constituent 1
Constituent 2
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
PHYSICO-CHEMICAL PROPERTIES
- Water solubility (under test conditions): 73 mg/l and corresponding pH = 3,8 (with pure water)
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48
Test conditions
- Test temperature:
- Room temperature
- pH:
- 7.5 to 7.9 from To to T48h
- Dissolved oxygen:
- 8.1 to 8.4 mg/L at To
7.4 to 8.1 mg/L at 48h - Nominal and measured concentrations:
- 8.0 - 4.6 - 2.7 - 1.6 - 0.9 - 0.55 - 0.34 - 0.19 - 0.11 - 0.061 - 0.039 - 0.023 mg/L and control
- Details on test conditions:
- TEST SYSTEM
- Type of flow-through (e.g. peristaltic or proportional diluter): static
- No. of organisms per vessel: 20
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST CONCENTRATIONS
- Spacing factor for test concentrations: square root of 3
- Range finding study: 65.7 - 25.6 - 7.3 - 2.6 - 0.73 - 0.26 - 0.073 mg/L
- Test concentrations: 8.0 - 4.6 - 2.7 - 1.6 - 0.9 - 0.55 - 0.34 - 0.19 - 0.11 - 0.061 - 0.039 - 0.023 mg/L - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate (K2Cr207)
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24
- Dose descriptor:
- EC50
- Effect conc.:
- 1.1
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% CI : 0.88 mg/L - 1.32 mg/L
- Duration:
- 48
- Dose descriptor:
- EC50
- Effect conc.:
- 0.13
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% CI: 0.10 mg/L - 0.17 mg/L
- Duration:
- 48
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.023
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: Williams' test
- Results with reference substance (positive control):
- - Results with reference substance valid? Results with reference substance, K2Cr2O7, are valids
- EC50/LC50: 1.0 mg/L - Reported statistics and error estimates:
- EC50 calculated using the Log-Probit statistical model (Toxcalc software)
NOEC calculated using the Willams' Test (Toxcalc software).
Any other information on results incl. tables
Main Studies :
T = 24 h |
|||||||||||||
Concentrations mg/L |
8.0 |
4.6 |
2.7 |
1.6 |
0.9 |
0.55 |
0.34 |
0.19 |
0.11 |
0.061 |
0.039 |
0.023 |
Control |
I |
0 |
1 |
1 |
1 |
2 |
4 |
4 |
5 |
5 |
5 |
5 |
5 |
5 |
II |
0 |
0 |
0 |
1 |
3 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
III |
0 |
0 |
0 |
1 |
3 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
IV |
0 |
0 |
1 |
2 |
2 |
4 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
Total moving |
0 |
1 |
2 |
5 |
10 |
18 |
19 |
20 |
20 |
20 |
20 |
20 |
20 |
Immobilisation rate (%) |
100% |
95% |
90% |
75% |
50% |
10% |
5% |
0% |
0% |
0% |
0% |
0% |
0% |
Analytic serial number: 2469
Dissolved O2at 8 mg/L: 8.0 mg/L
T = 48 h |
|||||||||||||
Concentrations mg/L |
8.0 |
4.6 |
2.7 |
1.6 |
0.9 |
0.55 |
0.34 |
0.19 |
0.11 |
0.061 |
0.039 |
0.023 |
Control |
I |
0 |
0 |
0 |
0 |
0 |
1 |
1 |
3 |
3 |
4 |
5 |
5 |
5 |
II |
0 |
0 |
0 |
0 |
1 |
0 |
0 |
0 |
3 |
4 |
3 |
5 |
5 |
III |
0 |
0 |
0 |
0 |
0 |
1 |
1 |
1 |
3 |
3 |
5 |
5 |
5 |
IV |
0 |
0 |
0 |
0 |
0 |
0 |
1 |
2 |
4 |
4 |
3 |
5 |
5 |
Total moving |
0 |
0 |
0 |
0 |
1 |
2 |
3 |
6 |
13 |
15 |
16 |
20 |
20 |
Immobilisation rate (%) |
100% |
100% |
100% |
100% |
95% |
90% |
85% |
70% |
35% |
25% |
20% |
0% |
0% |
Analytic serial number: 2469
Dissolved O2at 1.6 mg/L: 7.4 mg/L
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the experimental conditions:
* 24h - EC50 = 1.1 mg/L (CI 95% : 0.88 - 1.32 mg/L)
* 48h - EC50 = 0.13 mg/L (CI 95% : 0.10 - 0.17 mg/L)
* 48h - NOEC = 0.023 mg/L - Executive summary:
The short-term toxicity (48h) on invertebrate (Daphnia magna) of the test item Sepisol Fast Blue 2BR was assessed by determining the nominal effective concentration (EC50) at which 50% of the Daphnia are immobilized. The method followed the standard NF EN ISO 6341 under static condition.
48h-EC50 values and associated confidence limits were calculated using a Log-Probit statistical model (software Toxcalc). The NOEC value was determined by using the Williams' test (software toxcalc).
The test substance was prepared in distilled water to obtain a stock solution of 73 mg/L. Immobilisation and viable abnormalities were recorded at 24h and 48h as well as pH and dissolved oxygen.
Validity criteria were fulfilled and the study was considered as valid.
A preliminary assay was performed at the following concentrations: 65.7 - 25.6 - 7.3 - 2.6 - 0.73 - 0.26 - 0.073 mg/L
The main assay was performed at the following test concentrations: 8.0 - 4.6 - 2.7 - 1.6 - 0.9 - 0.55 - 0.34 - 0.19 - 0.11 - 0.061 - 0.039 - 0.023 mg/L + a control, in quadruplicate, with 20 daphnia per test and control groups.
Conclusion:
* 24h - EC50 = 1.1 mg/L (CI 95% : 0.88 - 1.32 mg/L)
* 48h - EC50 = 0.13 mg/L (CI 95% : 0.10 - 0.17 mg/L)
* 48h - NOEC = 0.023 mg/L
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