4-({[4-(acryloyloxy)butoxy]carbonyl}oxy)benzoic acid

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study in compliance with GLP-criteria.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2003

Report date:

2003

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

4-({[4-(acryloyloxy)butoxy]carbonyl}oxy)benzoic acid

EC Number:

608-403-9

Cas Number:

297132-04-8

Molecular formula:

C15H16O7

IUPAC Name:

4-({[4-(acryloyloxy)butoxy]carbonyl}oxy)benzoic acid

Details on test material:

- Name of test material (as cited in study report): 4-(4-Acryloyloxy-butoxycarbonyloxy)-benzoic acid
- Physical state: white solid
- Homogeneity: homogeneous
- Storage conditions: Store at room temperature under light exclusion, keep dry

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

The concentration control analyses were performed with the nominal concentrations of 0, 12.5, 25, 50, and 100 mg/L. At the start of the test (after 0 h) and at the end of the test (after 48 h) samples from vessels without daphnids were analysed.

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION:
All test concentrations were weight in seperately in M4 medium. The test concentrations were treated in an ultrasonic bath for approx. 5 minutes and then stirred for about 20 hours at 20 +/- 2 °C. Undissolved test substance was removed by centrifugation.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Source: The clone of Daphnia magna STRAUS 1820 used was supplied by the Institut National de Recherche Chimique Appliquée, France, in 1978. From this date on this clone was cultured and bred continuously in the Laboratory Experimental Toxicology and Ecology of BASF AG in Ludwigshafen.
- Age at study initiation (mean and range, SD): 2 - 24 hours

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

2.20 - 3.20 mmol/L

Test temperature:

19.7-20.2 °C

pH:

7.5 - 8.1

Dissolved oxygen:

8.2 - 9.3 mg/L

Nominal and measured concentrations:

- Nominal concentrations: 0 (control), 12.5, 25, 50 and 100 mg/L.
- Measured concentrations: Nominal concentrations of 0, 12.5, 25, 50 and 100 mg/L were analysed at t=0 and at t= 48 h.
- t=0: n.d., 12.78, 23.1, 49.8, and 93.5 mg/L
- t=48h: n.d. 11.92, 22.1, 47.8, and 90.8 mg/L

Details on test conditions:

TEST SYSTEM:
- Test vessel: flat bottom glass tubes (20 mL)
- Fill volume: 10 mL
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS:
- Source/preparation of dilution water: M4 medium (according to ISO 10706) was prepared. This M4 medium is a synthetic fresh water, which was used for culture and test purposes. This water was prepared on the basis of an ultrapure, demineralized water with a conductivity <0.05 µS/cm. After preparation the M4 medium is aerated for approximately 24 h until saturation with oxygen is reached.

OTHER TEST CONDITIONS:
- Photoperiod: 16 : 8 hours day-night regime
- Illumination: Artificial light, type warm white (e.g. OSRAM L58 W31)
- Light intensity: 1-8 µE/(m2·s) at a wave length of 400 - 750 nm

EFFECT PARAMETERS MEASURED:
- Visual determination of mobility after 0, 24 and 48 hours

Reference substance (positive control):

yes

Remarks:

potassiumdichromate

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

The EC50(24h) of the control substance potassium dichromate was 1.34 mg/L

Reported statistics and error estimates:

Due to the results of the test no statistical evaluation of data was performed.

Any other information on results incl. tables

Number of mobile Daphnids in the different test concentrations.

Conc. (mg/L)	Number of mobile daphnids after
	24 h	48 h
0	20	20
12.5	20	19
25	20	18
50	20	20
100	20	20