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Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Guideline study in compliance with GLP-criteria.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2003
Report date:
2003

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
4-({[4-(acryloyloxy)butoxy]carbonyl}oxy)benzoic acid
EC Number:
608-403-9
Cas Number:
297132-04-8
Molecular formula:
C15H16O7
IUPAC Name:
4-({[4-(acryloyloxy)butoxy]carbonyl}oxy)benzoic acid
Details on test material:
- Name of test material (as cited in study report): 4-(4-Acryloyloxy-butoxycarbonyloxy)-benzoic acid
- Physical state: white solid
- Homogeneity: homogeneous
- Storage conditions: Store at room temperature under light exclusion, keep dry

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
The concentration control analyses were performed with the nominal concentrations of 0, 12.5, 25, 50, and 100 mg/L. At the start of the test (after 0 h) and at the end of the test (after 48 h) samples from vessels without daphnids were analysed.

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION:
All test concentrations were weight in seperately in M4 medium. The test concentrations were treated in an ultrasonic bath for approx. 5 minutes and then stirred for about 20 hours at 20 +/- 2 °C. Undissolved test substance was removed by centrifugation.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Source: The clone of Daphnia magna STRAUS 1820 used was supplied by the Institut National de Recherche Chimique Appliquée, France, in 1978. From this date on this clone was cultured and bred continuously in the Laboratory Experimental Toxicology and Ecology of BASF AG in Ludwigshafen.
- Age at study initiation (mean and range, SD): 2 - 24 hours

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
2.20 - 3.20 mmol/L
Test temperature:
19.7-20.2 °C
pH:
7.5 - 8.1
Dissolved oxygen:
8.2 - 9.3 mg/L
Nominal and measured concentrations:
- Nominal concentrations: 0 (control), 12.5, 25, 50 and 100 mg/L.
- Measured concentrations: Nominal concentrations of 0, 12.5, 25, 50 and 100 mg/L were analysed at t=0 and at t= 48 h.
- t=0: n.d., 12.78, 23.1, 49.8, and 93.5 mg/L
- t=48h: n.d. 11.92, 22.1, 47.8, and 90.8 mg/L
Details on test conditions:
TEST SYSTEM:
- Test vessel: flat bottom glass tubes (20 mL)
- Fill volume: 10 mL
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS:
- Source/preparation of dilution water: M4 medium (according to ISO 10706) was prepared. This M4 medium is a synthetic fresh water, which was used for culture and test purposes. This water was prepared on the basis of an ultrapure, demineralized water with a conductivity <0.05 µS/cm. After preparation the M4 medium is aerated for approximately 24 h until saturation with oxygen is reached.

OTHER TEST CONDITIONS:
- Photoperiod: 16 : 8 hours day-night regime
- Illumination: Artificial light, type warm white (e.g. OSRAM L58 W31)
- Light intensity: 1-8 µE/(m2·s) at a wave length of 400 - 750 nm

EFFECT PARAMETERS MEASURED:
- Visual determination of mobility after 0, 24 and 48 hours
Reference substance (positive control):
yes
Remarks:
potassiumdichromate

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Results with reference substance (positive control):
The EC50(24h) of the control substance potassium dichromate was 1.34 mg/L
Reported statistics and error estimates:
Due to the results of the test no statistical evaluation of data was performed.

Any other information on results incl. tables

Number of mobile Daphnids in the different test concentrations.

Conc. (mg/L)

Number of mobile daphnids after

24 h

48 h

0

20

20

12.5

20

19

25

20

18

50

20

20

100

20

20

Applicant's summary and conclusion