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EC number: 202-259-7 | CAS number: 93-58-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 29 July 1988
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: This test is comparable to guideline study and was conducted according to OECD-Guidlines for testing of chemicals, 203 Fish, Acute Toxicity Test as adopted on 04 April 1984.
Data source
Reference
- Reference Type:
- other company data
- Title:
- Unnamed
- Year:
- 1 988
- Report date:
- 1988
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Methyl benzoate
- EC Number:
- 202-259-7
- EC Name:
- Methyl benzoate
- Cas Number:
- 93-58-3
- Molecular formula:
- C8H8O2
- IUPAC Name:
- methyl benzoate
- Test material form:
- other: liquid
- Details on test material:
- - Name of test material (as cited in study report): Methyl Benzoate (Benzoesauremethylester)
- Analytical purity: 98%
- Impurities (identity): 4-methylbenzaldehyde Acetophenon <2%, methyl p-toluate < 2%
Constituent 1
Sampling and analysis
- Analytical monitoring:
- not specified
Test solutions
- Details on test solutions:
- As dilution water was reconstituted water used according to ISO/DIS 7346/1 composition. The treatment was carried out in a system consisting of two steel basin with Hostalen® lining and a capacity of 700 liters each. The dilution water solution prepared was vented to the oxygen saturation. The experiment was carried out in a static system, the calibrated to 10 liters test container consisted of glass (30 cm long, 22 cm wide, 24 cm high) and were in a water bath made ¿¿of Hostalit Zr with a visible disk of plexiglass. The test substance was mixed with dilution water with the ultra-turrax and introduced ultrasonic homogenizing and stirring with a glass rod in the experimental basin.
Test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- The test organs were from the West Aquarium 3422 Bad Lauterberg.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
Test conditions
- Test temperature:
- 21,6 - 23.0 oC for exposed fish and 21.0 - 22.4 oC for controls
- pH:
- 6.9 -8.2 for exposed fish and 7.4 - 8.0 for controls
- Dissolved oxygen:
- 5.9 - 9.4 mg/L for exposed fish and 7.1 9.3 for controls
- Salinity:
- freshwater
- Nominal and measured concentrations:
- 0 (control), 5, 10, 25, 35, and 50 mg/l
- Details on test conditions:
- TEST SYSTEM
The temperature was measured with oxygen meter (OXI 196 mte) and oxygen electrode (EO 196 1 1.5). Oxygen content was measured with electronic measuring oxygen (meter oxi 196). The pH was measured with an pH meter( ph 763) electronic measurement. The test substance was mixed with dilution with ultra turrax and ultrasonic homogenizing and stir with a glass rod placed in the experimental tank. The testing solution with the exception of the 10 mg / L group, had a slight turbidity at 25 and 35 mg / L.
On presentation of the proof concentrations and subsequent measurement of water parameters per Pruf and control pool of 10 fish were used randomly. Throughout the experimental period, the fish were not fed.
After 2 -4, 24, 48, 72 and 96 hours is an inspection of the fish was the lethality and cause visible changes in the appearance and behavior were recorded and dead fish were removed from the pool. The fish was removed if no respiratory movements were more visible and he no longer even on slightly mechanical irritant response. On the same date the water parameters were measured and logged as well.
Since the factor of the square root of concentration levels 2 was no other concentrations were gepruft. Was given the experimentally determined value of the LC50 .
The holder was over a period of at least 14 days before trial begins in evaporating water planning under the following conditions.
Immediately before commencing trial was representative of the batch of fish used jeweil 10 fishing korperlange determined. these animals were not used for the experiment.
oxygen content: >80%
Temperature: 22 + or - 1 oC
light duration 12 hours day.
Stock density: < or equal to 1 gram fish/liter water
Feeding: ad Libitum - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- >= 35 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 35 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 48 h
- Dose descriptor:
- LC100
- Effect conc.:
- 50 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 50 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
At 5 mg /L there was protruding gill. In the concentration range of 10 to 50 mg /L fish showed the following symptoms: increased respiratory rate and respiratory movements. Activity decreased to elevated terrible reaction swim or stand at the waser upper flat and stagger on pelvic floor isolated in the side or supine position gyroscopes. The dead fish were lightened to dark discolored.- Results with reference substance (positive control):
- no data
- Reported statistics and error estimates:
- probit analysis was not possible due to the present mortality
Any other information on results incl. tables
- Sublethal observations / clinical signs:
- Under the described experimental conditions BME had the following effects after 48and96 hours: Concentration 48 hours 48 hours 96 hours 96 hours (mg/l) absolut relative (%) absolute relative (%)
0 0/20 0 0/20 0 5 0/10 0 0/10 0 10 0/10 0 0/10 0 25 0/10 0 0/10 0 35 4/10 40 5/10 50 50 10/10 100 10/10 100
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- 96-hr LC50 of BME is 35 mg/l.
- Executive summary:
The acute toxicity of BME of 98% purity was tested on Zebra fish (Danio rerio) for 96 hours according to OECD 203 testing guidelines. A stock solution was prepared in water and pH-adjusted to 6.9 - 8.2. The LC50 was 35 mg/L. Probit analysis was not possible due to the present mortality and the study is considered as valid with restrictions
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