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EC number: 689-137-0 | CAS number: 247569-56-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- January 2011 - October 2012
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: This study has been performed according to OECD and/or EC guidelines and according to GLP principles.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 012
- Report date:
- 2012
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 2-fluoro-5-methanesulfonylbenzoic acid
- EC Number:
- 689-137-0
- Cas Number:
- 247569-56-8
- Molecular formula:
- C8 H7 F O4 S
- IUPAC Name:
- 2-fluoro-5-methanesulfonylbenzoic acid
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
- Details on test material:
- Description White solid
Purity 99.5%
Batch number BS11020465
BMG Substance number M1204-02810-01
CAS number 247569-56-8
Date received 18 June 2012
Expiry date 21 May 2013
Storage conditions Room temperature in the dark
Constituent 1
Method
- Target gene:
Salmonella typhimurium
Strains Genotype
TA1537 his c 3076; rra-; uvra-:
Type of mutations indicated
frame shift mutations
TA98
TA1535
TA100
Escherichia coli
his D 3052; rfa-; uvrB-;R-factor his G 46; rfa-; uvra-:
his G 46; rra-; uvra-; R-factor
base-pair substitutions
Strain Genotype
WP2uvrA trp-; uvrA:
Type of mutations indicated
base-pair substitution
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- E. coli WP2 uvr A
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- N-ethyl-N-nitro-N-nitrosoguanidine
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- not determined
- Cytotoxicity / choice of top concentrations:
- not determined
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
The test item, 5-Methylsulfonyl-2-fluorobenzoic acid R04638493, was considered to be non-mutagenic under the conditions of this test. - Executive summary:
Introduction
The test method was designed to be compatible with the guidelines for
bacterial mutagenicity testing published by the major Japanese Regulatory Authorities including
METI, MHLW and MAFF, the OECD Guidelines for Testing of Chemicals No.
471 "Bacterial Reverse Mutation Test", Method 813/14 of Commission Regulation (EC) number 440/2008
of 30 May 2008 and the USA, EPA (TSCA) OPPTS harmonised guidelines.
Methods
Salmonella typhimurium strains TA1535, TA1537, TA98, TA100 and Escherichia coli strain
WP2uvrA were treated with the test item, 5-Methylsulfonyl-2- fluorobenzoic acid R04638493, using
both the Ames plate incorporation and pre incubation methods at up to six dose levels, in
triplicate, both with and without the addition of a rat liver homogenate metabolising system (10%
liver S9 in standard co factors). The dose range for the first experiment was determined in a
preliminary toxicity assay and was 50 to 5000 microg/plate. The experiment was repeated on a
separate day (pre-incubation method) using an amended dose range (15 to 5000 microg/plate}, fresh
cultures of the bacterial strains and fresh test item formulations.
An additional dose level and an expanded dose range were selected in Experiment 2 in order to
achieve both four non-toxic dose levels and the potential toxic limit of the test item following
the change in test methodology.
Results
The vehicle (dimethyl sulphoxide) control plates gave counts of revertant colonies
generally within the normal range. All of the positive controls used in the test induced marked
increases in the frequency of revertant colonies, both with or without metabolic activation. Thus,
the sensitivity of the assay and the efficacy of the 59-mix were validated.
In the first experiment (plate incorporation method), the test item caused no visible reduction in
the growth of the bacterial background lawns of any of the tester strains except for Salmonella
strain TA1537 which exhibited weakened lawns at 5000 microg/plate in the absence of S9-mix only. In
Experiment 2 (pre-incubation method) there was no evidence of toxicity to any tester strain at any
test item dose in either the absence or presence of S9-mix. The test item was tested up to the
maximum recommended dose level of 5000 microg/plate. No test item precipitate was observed on the plates at any of the doses
tested in either the presence or absence of S9.
No significant increases in the frequency of revertant colonies were recorded for any of the
bacterial strains, with any dose of the test item, either with or without metabolic activation or
exposure method.
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