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EC number: 204-634-0 | CAS number: 123-54-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in mammalian cells
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Meets generally accepted scientific standards, well documented and acceptable for assessment.
Data source
Referenceopen allclose all
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 986
- Report date:
- 1985
- Reference Type:
- secondary source
- Title:
- Unnamed
- Year:
- 2 001
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 476 (In Vitro Mammalian Cell Gene Mutation Test)
- Principles of method if other than guideline:
- Preliminary trials were performed to determine an appropriate range of test concentration in which the highest concentration would kill no more than 90 % of the treated CHO cells. In this preliminary experiment concentrations above 2.0 mg/mL in the presence of S9 and 3.0 mg/mL in the absence of S9 virtually killed all cells. All incubations were run in duplicate. Cells were exposed to at least five concentrations which allowed sufficient cell survival for assessment of survival and quantification of mutants. Cells were exposed for 5 h in tests both with and without metabolic activation. The mutant fraction was determined after a 9 to 12 day subculturing period to allow expression of the mutant phenotype. Metabolic activation was applied by S9 liver homogenate, prepared from Aroclor 1254-induced, Sprague-Dawley male rats.
- GLP compliance:
- yes
- Type of assay:
- mammalian cell gene mutation assay
Test material
- Details on test material:
- Purity: 99.2%
Lot No.: S-050082
Constituent 1
Method
- Target gene:
- HPRT
Species / strain
- Species / strain / cell type:
- Chinese hamster Ovary (CHO)
- Details on mammalian cell type (if applicable):
- CHO-K1-BH4 (subclone D1)
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9-Mix from livers of Sprague-Dawley rats pretreated with Aroclor 1254
- Test concentrations with justification for top dose:
- Without S9-mix: 0.005-1.5 mg/mL
Wit S9-mix: 0.005-1.0 mg/mL
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: ethylenemethanesulfonate (200 µg/mL)
- Details on test system and experimental conditions:
- DURATION
- Exposure duration: 5 h
- Expression time (cells in growth medium): 9-12 days
NUMBER OF REPLICATIONS: incubations were run in duplicates
SURVIVING FRACTION:
Determination 18-24 hrs after removal of the test chemical using 4 plates/culture and 100 cells per plate.
MUTANT FRACTION:
200000 cells per plate in 5 plates per dose in selective medium.
Plating efficiency in non-selective medium: 4 plates/dosed culture with 100 cells/plate.
DETERMINATION OF CYTOTOXICITY
- Method:
other: Preliminary test where number of live cells was investigated (no data about applied method given) - Statistics:
- After tranformation of the mutation frequencies (MF) according to the conversion method of Box and Cox (1964), CHO data were analyzed according to Snee and Irr (Snee, R .D. und J .D . Irr, Mutation Research . 85 (1981), 77-93).
Results and discussion
Test results
- Species / strain:
- Chinese hamster Ovary (CHO)
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- other: Only data from a preliminary test are available. The concentrations used should give at least 10% survival at the highest concentration used.
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Additional information on results:
- 2,4-Pentanedione did not produce any reproducible or statistically significant increases in the incidences of mutations of CHO cells at concentrations between 0.005 to 1.5 mg/mL in tests without an S9 metabolic activation system or from 0.005 to 1.0 mg/mL with S9-mix. Random cultures with increased mutant values were within the typical range of variability for this test in the investigating laboratory and the increases were not reproducible in the duplicate cultures/dose level. 2,4-Pentanedione was not considered to be an active gene mutagen under the conditions of the CHO test system.
Applicant's summary and conclusion
- Conclusions:
- Under the evaluated conditions no increased mutation frequency was observed. The result was evaluated to be negative.
- Executive summary:
2,4-Pentanedione did not produce any reproducible or statistically significant increases in the incidences of mutations of CHO cells at concentrations between 0.005 to 1.5 mg/mL in tests without an S9 metabolic activation system or from 0.005 to 1.0 mg/mL with S9-mix. Random cultures with increased mutant values were within the typical range of variability for this test in the investigating laboratory and the increases were not reproducible in the duplicate cultures/dose level. 2,4-Pentanedione was not considered to be an active gene mutagen under the conditions of the CHO test system.
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