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EC number: 835-183-3 | CAS number: 83420-16-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch number of test material: 190701
- Expiration date of the lot/batch: 24 July 2021
- Purity: >90%
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Room Temperature
- Stability under storage conditions: Stable - Analytical monitoring:
- yes
- Remarks:
- HPLC method
- Vehicle:
- yes
- Remarks:
- using an acidified water (phosphoric acid 0.1%)
- Details on test solutions:
- As dilution water, reconstituted water has been used; it has been prepared dissolving thefollowing salts in 1 litre of deionised water:
N. SOLUTION REAGENTS FORMULA QUANTITY(g/L)
A Bihydrated calcium chloride CaCl2 2H2O 11.76
B Eptahydrated magnesium sulphate MgSO, 7H20 4.93
C Acid sodium bicarbonate NaHCO3 2.59
D Potassium chloride KCl 0.23
25 mL of solutions+ A, B, C and D have been taken to 1000 mL volume with deionised water.
Medium has been prepared according to the same proportion and reported on internal logbook. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Test organism: Daphnia magna straus, Crustacean, Cladocero
Age: 6-24 hours
Origin: Breederin Eurofins Biolab Test Facility
N. of animals: 20 for each treated — 20 for control — 20 for control acidified
N. of animals/beaker: 5
N. of replications: 4 for control and 4 for each treated tested concentration
N. of concentrations: 5 for range finding test and ECs, test - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- between 140 and 250 mg CaCO3/L
- Test temperature:
- 20°C + 2°C
- pH:
- Group of control acidiied: 7.75-7.89
Group of control: 8.04-8.09
Group of treated: 7.83-7.95 - Dissolved oxygen:
- > 3 mg/L
- Nominal and measured concentrations:
- nominal concentration:1.00mg/L, 0.45mg/L,0.21mg/L, 0.09mg/L, 0.04mg/L, 0.00mg/L, 0.00mg/L(acidified).
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass Beaker (500 ml).
- Type of flow-through (e.g. peristaltic or proportional diluter): static
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): 4
OTHER TEST CONDITIONS
- Light intensity: None during the course of the test.
VEHICLE CONTROL PERFORMED: yes
RANGE-FINDING STUDY
- Test concentrations: 1000 mg/L, 100 mg/L, 10 mg/L, 1 mg/L and 0.1 mg/L
- Results used to determine the conditions for the definitive study:
The test item in the solutions was not well solubilized and analytical chemical results (EXC LV - 20AA6307) confirmed. A new stock solution (20 mg/L) has been prepared using an acidified water (phosphoric acid 0.1%). Then 5 dilutions have been performed with culture medium. Two controls have been prepared: one with culture medium and onewith culture medium acidified.
The test has been performed on the diluted nominal solutions: 0.04, 0.09, 0.21, 0.45, 1.00 mg/L. - Reference substance (positive control):
- no
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.042 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- The sample is considered not stable because the measured concentration of the test item at the end of the test has not been maintained within + 20 % to the concentration at the measured concentrations at the
beginning of the test.
The test item resulted not stable during the test, therefore the statistical analysis (EC50) has been performed usingtheinitial measured test concentration as required by the guideline OECD 202.
For the two lower concentrations a protective factor has been used. The lower percentage recovery (55.6%) related to analytical chemical results has been used.
Validity criteria have been satisfied:
% of immobilized Daphnie in control: 0. - Validity criteria fulfilled:
- yes
- Executive summary:
The aim of the study is to determine the ecotoxicological effects of the test item “N4,N4’-HEXANE-1,6 - DIYLBIS[N-BUTYL-6-CHLORO-N,N’-BIS(2,2,6,6-TETRAMETHYLPIPERIDIN-4-YL)- 1,3,5-TRIAZINE- 2,4-DIAMINE]”on biotic systems: in particular aquatic organism such as Daphnia magna was used as
test system in order to calculate the EC50 value, according to OECD guideline N. 202.
After a range finding test, the organisms have been exposed to 5 differentdilutions of the test item for a total period of 48 hours.
Daphnia have been kept under observations for a period of 48 hours and the number of immobilised organisms and/or possible abnormal behaviours both in control and in treatment group vessel have been observed.
At the beginning and at the end of the test (48 hours) dissolved oxygen and pH have been measuredin the highest concentration and in control group. Temperature of the assay environment have been recorded at the beginning and at the end of the test.
Stability of the test item in the definitive test has been performed using HPLC technique equipped with UV detector
The test item has been measured at the beginning and at the end of the test in the control and in the treated samples.
The obtained results, in compliance with test acceptability criteria, EC50 of the test item at T= 48 h (measured concentration) = 0.042 mg/L
Reference
Description of key information
The obtained results of OECD 202, in compliance with test acceptability criteria, EC50 of the test item at T= 48 h (measured concentration) = 0.042 mg/L
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 0.042 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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