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Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2006
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2006
Report date:
2006

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
Deviations:
no
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
Deviations:
no
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
2-({(5S)-2-Oxo-3-[4-(3-oxomorpholin-4-yl)phenyl]-1,3-oxazolidin-5-yl}methyl)-1H-isoindole-1,3(2H)-dione
EC Number:
610-201-0
Cas Number:
446292-08-6
Molecular formula:
C22 H19 N3 O6
IUPAC Name:
2-({(5S)-2-Oxo-3-[4-(3-oxomorpholin-4-yl)phenyl]-1,3-oxazolidin-5-yl}methyl)-1H-isoindole-1,3(2H)-dione

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Details on inoculum:
Test Organisms
Type: mixed population of aquatic microorganisms (activated sludge)
Origin: aeration tank of a wastewater plant treating predominantly domestic sewage (Wupper area water authority, STP Odenthal)
Date of collection: 06 Mar 2006
Pre-treatment: none
Concentration of inoculum: 30 mg/L ss
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
100 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
O2 consumption
Details on study design:
A measured volume of inoculated mineral medium, containing a known concentration of the test chemical (usually 100 mg/Litre), to give at least 50-100 mg ThOD/L as the nominal sole source of organic carbon, is stirred in a closed flask at a constant temperature
(22 ± 2°C) for up to 28 days. The consumption of oxygen is determined by measuring the quantity of oxygen (produced electrolytically) required to maintain constant gas volume in the respirometer flask. Evolved carbon dioxide is absorbed in a solution of potassium hydroxide. The amount of oxygen taken up by the test chemical (corrected for uptake by blank inoculum, run in parallel) is expressed as a percentage of theoretical oxygen demand (ThOD) or chemical oxygen demand (COD).

Exposure conditions
-Test volume: 250 mL
-Test apparatus: Oxi Top System (WTW)
-Mixing : 1magnetic stirrer per test vessel
-28 days incubation at 22 +/- 1°C
Reference substance
Reference substance:
benzoic acid, sodium salt
Remarks:
purity 99%

Results and discussion

% Degradation
Key result
Parameter:
% degradation (O2 consumption)
Value:
3
Sampling time:
28 d

BOD5 / COD results

Results with reference substance:
A degradation (mean) of 91% after 28 days was determined for the reference compound.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Remarks:
The reference compound has reached the level for ready biodegradability by 14 days. No toxicity of the test item has been observed in the toxicity control. The oxygen uptake of the inoculum blank is <= 60 mg/L.
Interpretation of results:
not readily biodegradable
Conclusions:
Not Readily Biodegradable: 3% after 28 d
Executive summary:













The biodegradation of Hydroxyaminophthalimid has been investigated according to OECD 301F. Activated sludge from a domestic sewage treatment plant was used. After an exposure of 28 d a biodegradation of 3% was observed. Thus, the test item is not readily biodegradable according to the OECD criteria. No inhibition of the activated sludge was recorded in the toxicity control. Thus, the test is considered valid.