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Diss Factsheets
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EC number: 946-448-9 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2013-12-12 until 2014-01-02 and 2014-01-16 until 2014-02-27
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: The data are derived from work involving two non-GLP OECD 301F studies with the aim to minimize inhibition of the inoculum by means of testing several concentrations of the test item and using silica gel to enable biodegradation.
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- other: OECD 301F
- GLP compliance:
- no
- Remarks:
- The laboratory complies the general requirements for the competence of testing and calibration laboratories.
- Analytical monitoring:
- not required
- Vehicle:
- no
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- - Source of activated sludge: Escatawpa Wastewater Treatment Plant (ESC), Mississippi. secondary biological wastewater treatment system utilizing the activated sludge process.
- Laboratory culture: not applicable
- Method of cultivation: not applicable
- Storage conditions: not applicable
- Storage length: not applicable (settling immediately started)
- Preparation of inoculum for exposure:
The sludge after arrival is settled and decanted with mineral water added back. This settling procedure is repeated twice more and aerated over night prior to use. The day of the test, the air is turned off and inoculate settled in an Imhoff cone. The settled concentrate is drained into beaker and solids concentration determined.
- Concentration of sludge: 30mg/L of suspended solids (SS) - Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 1 d
- Test temperature:
- 22±1 °C
- Nominal and measured concentrations:
- 10, 20 and 40 mg/L
- Details on test conditions:
- TEST CONDITIONS
- Composition of medium: according to OECD 301F guideline
- Additional substrate: no (test 1December 2013) and yes (silica gel in test 2 January 2014).
- Solubilising agent (type and concentration if used): In test 2, approximately 500 mg of silica gel (Aldrich, Merck grade 9385) with a mesh size of 230-400 angstrom was weighed out and added to all test bottles (including blanks, reference compound and test material) after addition of the test compound and before the inoculate concentrate is added.
- Test temperature: 22±1 °C
- pH: not specified
- Suspended solids concentration: 30 mg/L
- Continuous darkness: not specified
TEST SYSTEM
- Culturing apparatus: 1-liter vessels
- Number of culture flasks/concentration: triplicates for test 1 and quadruplicates in test 2
- Measuring equipment: Coordinated Environmental Services (CES) Ltd (Cornwell, UK) aerobic electrolytic respirometer unit consisting of 20 one-liter
vessels fitted with heads containing O2 and CO2 sensors.
- Measurement frequency: CO2 generation and O2 uptake data is recorded by the instrument every six hours during the 28-day test.
- Other: Stirring is adjusted to 300-400rpm.
CONTROL AND BLANK SYSTEM
- Inoculum blank: Blank 1-liter vessels are prepared identical to the samples (i.e. test medium, inoculate, and silica gel when present).
- Reference substance and control: Sodium benzoate, silica gel when present is also included in the reference 1-liter vessels.
- Abiotic sterile control: not applicable
- Toxicity control: no - Reference substance (positive control):
- yes
- Remarks:
- benzoic acid, sodium sal
- Key result
- Duration:
- 6 h
- Dose descriptor:
- IC50
- Effect conc.:
- 10.97 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Key result
- Duration:
- 12 h
- Dose descriptor:
- IC50
- Effect conc.:
- 11.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Key result
- Duration:
- 18 h
- Dose descriptor:
- IC50
- Effect conc.:
- 13.28 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Key result
- Duration:
- 24 h
- Dose descriptor:
- IC50
- Effect conc.:
- 14.54 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Conclusions:
- The concentrations of Benzenesulfonic acid, 4-C15-16-sec-alkyl derivs. required to inhibit oxygen uptake by 50% were 10.97 mg/L, 11.70 mg/L, 13.28 mg/L and 14.54 mg/L at 6, 12, 18 and 24 hours, respectively. The mean IC50 for the 24 hour time period was 12.62 mg/L.
- Executive summary:
As an add-on to an OECD 301F ready biodegradability study, the respiratory inhibition potential of Benzenesulfonic acid, 4-C15-16-sec-alkyl derivs. was measured. All concentrations of the substance tested produced some inhibition of oxygen uptake. The concentrations of Benzenesulfonic acid, 4-C15-16-sec-alkyl derivs. required to inhibit oxygen update by 50% were 10.97 mg/L, 11.70 mg/L, 13.28 mg/L and 14.54 mg/L at 6, 12, 18 and 24 hours, respectively.
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline Study without detailed documentation
- Principles of method if other than guideline:
- Method: other
- GLP compliance:
- not specified
- Analytical monitoring:
- not specified
- Test organisms (species):
- activated sludge
- Duration:
- 30 min
- Dose descriptor:
- EC50
- Effect conc.:
- > 261 mg/L
- Conclusions:
- The 30 minute EC50 of MIPA to activated sludge respiration was greater than 261 mg/L.
Referenceopen allclose all
Test item |
respiration |
% of blank |
[mg/L] |
[mg O2/g TS] |
|
1.3 |
12 |
115 |
3.9 |
12 |
115 |
13 |
13 |
125 |
39 |
16 |
154 |
130 |
18 |
173 |
261 |
11 |
106 |
The test item does not inhibit respiration
Description of key information
The concentrations of the read across substance Benzenesulfonic acid, 4-C15-16-sec-alkyl derivs. required to inhibit oxygen uptake by 50% were 10.97 mg/L, 11.70 mg/L, 13.28 mg/L and 14.54 mg/L at 6, 12, 18 and 24 hours, respectively.
The mean IC50 for the 24 hour time period was 12.62 mg/L.
For the second read across substance (MIPA), the 30 minute EC50 to activated sludge respiration was greater than 261 mg/L.
As the toxicity to microorganisms value for Benzenesulfonic acid, 4 -C15 -16 -sec-alkyl derivs. was significantly lower than that for MIPA, it will be used for Benzenesulfonic acid, 4 -C15 -16 -sec-alkyl derivs. MIPA-salt.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 12.62 mg/L
Additional information
Due to suspected microorganism toxicity, the respiratory inhibition potential of Benzenesulfonic acid, 4-C15-16-sec-alkyl derivs. was measured as an add-on to an OECD 301F ready biodegradability study. All concentrations of the substance tested produced some inhibition of oxygen uptake. The concentrations of Benzenesulfonic acid, 4-C15-16-sec-alkyl derivs. required to inhibit oxygen update by 50% were 10.97 mg/L, 11.70 mg/L, 13.28 mg/L and 14.54 mg/L at 6, 12, 18 and 24 hours, respectively. The mean IC50 for the 24 hour time period was 12.62 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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