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EC number: 600-872-8 | CAS number: 108419-35-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 03 May 1985 to 14 June 1985
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 985
- Report date:
- 1985
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: EPA Chemical Fate Test Guidelines, publ. No. EPA 560/6-83-003, Method CG-2000 (Aerobic Aquatic Biodegradation) 1982.
- Deviations:
- not specified
- GLP compliance:
- yes
- Remarks:
- Good Laboratory Practice Regulations set forth in 40 CFR Part 792.
Test material
- Reference substance name:
- Acetic acid, C11-14-isoalkyl esters, C13-rich
- EC Number:
- 283-740-9
- EC Name:
- Acetic acid, C11-14-isoalkyl esters, C13-rich
- Cas Number:
- 84712-50-5
- Molecular formula:
- C15H30O2
- IUPAC Name:
- Acetic acid, C11-14-isoalkyl esters, C13-rich
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Expiration date of the lot/batch: Batch I
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Stability under test conditions: The stability of the test material with regard to aquatic biodegradation is the subject of this report.
FORM AS APPLIED IN THE TEST (if different from that of starting material): colorless liquid (not different)
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- other: mixture of natural sediment (freshwater) and activated sludge (domestic)
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): sediment: pond sediment was obtained as a dredge sample from Colonial Park, East Millstone, New Jersey; activated sludge: sludge was obtained fresh from the Raritan Valley-Somerset Sewage Authority, Bridgewater, New Jersey.
- Method of cultivation: Acclimation Phase (May 3, 1985 to May 17, 1985): The acclimation medium was prepared by adding the following to a 2L Erlenmeyer flask: 1 mL each of mineral salt Solutions I, II, and III, 10.0334 g of sediment, 100 mL of activated sludge, 900 mL of glass distilled water, 25.7 mg casamino acids and 25.9 mg yeast extract. The mixture was homogenized in a Waring blender for 5 minutes and then filtered through glass wool. On days 0, 7, and 11; 9, 10 and 10 µL (specific gravity = 0.87 @ 20°C) of MRD-84-521, respectively, were added to the acclimationnmedium. The acclimation medium was shaken gently on the
gyrorotatory shaker for 13 days in the dark. The Erlenmeyer flask was stoppered with gauze which was removed only at times of test material addition.
- Storage conditions: dark
- Storage length: 13 days
- Preparation of inoculum for exposure: On day 13 the contents of the acclimation medium flask were poured into an Imhoff settling cone and were allowed to settle overnight. The resulting supernatant served as the inoculum for the biodegradation phase test systems. - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- >= 12.2 - <= 13 mg/L
- Based on:
- test mat.
- Details on study design:
- TEST CONDITIONS
- Composition of medium: 1 mL each of mineral salt Solutions, 100 mL of adapted inoculum and 900 mL of aerated glass distilled water.
- Mineral Salt Solutions: I: NH4CI (35 g/L), KNO3 (15 g/L), K2HPO4 x 3H2O (76 g/L), NaH2P04 x H20 (26 g/L); II: KCl (10 g/L), MgS04 (20 g/L), FeS04 x 7H20 (1 g/L); III: CaCl2 (5 g/L), ZnCl2 (0.05 g/L), MnCl2 x 4H20 (0.5 g/L), CuCI2 x 2H2O (0.07 g/L), CoCl2 x 6H20 (0.003 g/L), H3BO3 (0.001 g /L), MoO3 (0.0004 g/L)
- Test temperature: 21.5 - 25 °C
- pH adjusted: no
- Aeration of dilution water: yes
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: 2 L Gledhill flasks
- Number of culture flasks/concentration: 3 replicates
- Method used to create aerobic conditions: At each sampling point the flask contents were sparged with air through the sidearms for 5 minutes.
- Measuring equipment: As more carbonate is formed, the trap solution becomes more acidic so that less HCI titrant is required to reach the equivalence point.
- Test performed in closed vessels: The eight flasks were sealed.
- Details of trap for CO2 and volatile organics if used: 10 mL of 0.2N sodium hydroxide (NaOH) were added to the center reservoir of each flask.
SAMPLING
- Sampling frequency: Day 4, 7, 11, 14, 18, 21, 25 and 28.
- Sampling method: The flasks were removed from the shaker and the spent NaOH was emptied from the reservoirs, combined with 2x10 mL rinsings of distilled water into 125 mL Erlenmeyer flasks. A few drops of phenolphthalein indicator were added to the spent solutions and they were titrated against standardized 0.1 N hydrochloric acid (HCI, standardized 0.101 N HCI was also used) to a clear endpoint. The amount of HCI required to reach the endpoint was recorded. Ten mL of fresh 0.2N NaOH were added to each reservoir and the flask contents were sparged with air through the sidearms for 5 minutes. The flasks were resealed with rubber septums and they were returned to the gyrorotatory shaker and agitated in the dark.
Day 28: The flask contents were acidified to a pH of less than 3 with concentrated H2SO4 and agitated on the shaker for four more hours. Ten mL TOC sample aliquots were removed from the flask contents and analyzed for total organic carbon (TOC). The flasks were then removed from the shaker and the reservoir contents were treated as on day 4, 7, 11, ... etc.
- Sample storage before analysis:
- Other:
CONTROL AND BLANK SYSTEM
- Inoculum blank: 3 replicates
- Toxicity control: Toxicity control: Positive control Phthalic acid: 103.8 mg/L and negative Control HgCl2: 51 g/L
STATISTICAL METHODS:
Reference substance
- Reference substance:
- other: phthalic acid
Results and discussion
- Preliminary study:
- no
% Degradation
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 31
- Sampling time:
- 28 d
- Details on results:
- The CO2 evolution rate for test material mineralization was found to be 1.5 x 10^-2/day, which corresponds to a biodegradation half-life (t1/2) of 46.2 days.
BOD5 / COD results
- Results with reference substance:
- Loss of phthalic acid from the positive control flask: 43.4 % based on CO2 evolution; 100.7 % based on TOC removal (28 days)
The results based on CO2 evolution are lower than those based on TOC removal for the positive control. This difference is probably a result of the incorporation of carbon into biomass. This theory is substantiated by findings that mineralization is clearly favored under the conditions of a high inoculum and this procedure incorporates a fairly low inoculum.
The data for the negative control flask indicates that the presence of HgCl2 inhibited degradation.
Any other information on results incl. tables
Table 1: Amount (mg) of Carbon evolved as Carbon Dioxide (1)
Test system |
Day 4 |
Day 7 |
Day 11 |
Day 14 |
Day 18 |
Day 21 |
Day 25 |
Day 28 |
Mean – Test (2) |
0.12 |
0.52 |
0.72 |
0.41 |
0.61 |
0.20 |
0.25 |
0.14 |
Cumulative Test |
0.12 |
0.64 |
1.36 |
1.77 |
2.38 |
2.58 |
2.83 |
2.97 |
Positive Control – Phthalic acid |
1.32 |
0.68 |
0.15 |
- (3) |
0.15 |
0.04 |
0.01 |
0.04 |
Cumulative Positive Control |
1.32 |
2.00 |
2.15 |
2.15 |
2.30 |
2.34 |
2.35 |
2.39 |
Negative Control – HgCl2 inhibited |
- |
- |
- |
- |
- |
- |
- |
- |
Cumulative negative Control |
- |
- |
- |
- |
- |
- |
- |
- |
Temperature °C |
25.0 |
22.0 |
23.0 |
22.0 |
22.0 |
21.5 |
21.5 |
22.5 |
(1) Calculated using the mean daily control values (Appendix II)
(2) Mean from Test A, B, and C daily values
(3) CO2 evolved was less than the mean daily control values
Table 2: Total carbon, total carbon dioxide evolution, percent material degradation, and percent TOC removal
Flask Solutions |
TOC day 1 (mg/L) |
TOC day 28 (mg/L) |
Cumulative carbon evolved (mg) as CO2 day 28 (Table 1) |
Percent material degraded day 28 |
Percent TOC removal day 28 |
Positive Control – Phthalic acid |
6.436 |
0.714 |
2.39 |
43.4 % (b) |
107 % |
Negative Control – HgCl2 inhibited |
6.545 |
5.737 |
0.0 |
0.0 % (b) |
11.3 % |
Mean Control |
0.924 |
0.750 |
----- |
|
|
|
Test substance added (mg) |
Carbon added (mg) (a) |
|
||
Mean – Test |
13.0 |
9.6 |
2.97 |
31 % (c) |
|
(a) Calculated as: Test substance added (mg) x 0.74 = carbon added (mg)
(b) Eq. 1
(c) Eq. 1a
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not specified
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- These results suggest that the biodegradation of the test substance was moderate under the conditions of the test. The substabce is not considered as rapidly biodegradable according to Regulation (EC) No. 1272/2008.
- Executive summary:
Based on the test results (31 % based on CO2 evolution) for ready biodegradation, the substance was determined moderately biodegradable after 28 d. The OECD criteria for ready biodegradation are not fulfilled.
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