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Diss Factsheets
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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Deviations:
- no
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- not applicable
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Remarks:
- HPLC/MS/MS analysis
- Details on sampling:
- Sampling schedule:
- Control: at 24 hours (2018-03-02)
- Test concentrations: on 2018-02-26, 2018-02-27, 2018-02-28, and 2018-03-01 freshly prepared media
and on 2018-02-27, 2018-02-28, 2018-03-01 and 2018-03-02 in the media after 24 hours of exposure - Vehicle:
- no
- Remarks:
- Auxiliaries: ultra sonic bath, magnet stirrer folded filter and antiseptic filter
- Details on test solutions:
- Pre-treatment of test item and preparation of test item concentrations:
- 500 mg of the test item were added to 5 litres of dilution water, treated for 60 seconds at 8000 rpm with an ultra turrax and stirred for 24 h on a magnetic stirrer
- Undissolved particles of the test item were removed by filtration using a folded filter with a pore size of 7-12 µm and an aseptic filter Sartobran sterile capsules (pore size of 0.45 µm + 0.2 µm) - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- - Source: Interaquaristik.de Shop (Germany)
- Date supplied: 2018-01-23
- Acclimatisation: Stock held since 2018-01-23 and acclimatised to the test conditions since then.
- Temperature: 20 - 24 °C
- Dissolved oxygen: > 5 mg/L
- Feeding: Commercial fish food, daily. Feeding discontinued 24 h prior to test start.
- Mortalities during acclimatisation period: < 5 %
- Medication: none
- Mean standard length (n = 14): 3.34 cm (S.D. = 0.12 cm)
- Holding and dilution water: Reconstituted water prepared according to the recommendations of ISO 7346 (ISO water) - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- 14.0 °dH (= 250 mg/L CaCO3)
- Test temperature:
- 22.3 °C (after 96 hours)
- pH:
- 7.7 (after 96 hours)
- Dissolved oxygen:
- 8.1 mg/L (after 96 hours)
- Salinity:
- n.a.
- Conductivity:
- No data
- Nominal and measured concentrations:
- - Test concentration (nominal): 100 mg/L
- Test concentration after 96 hours (HPLC/MS/MS values): 90.749 µg/L - Details on test conditions:
- EXPOSURE CONDITIONS:
- Test vessels: glass aquaria holding 5 L of test media covered by glass plates
- Experimental design: 1 test concentration plus 1 control
- Number of test organism: 7 fish per test concentration
- Feeding: none during the exposure period
- Method of initiation: fish were placed in prepared media
- Loading: 0.69 g body weight (wet weight) per litre
- Photoperiod: 16 h light : 8 h dark
- Temperature: 21.7 to 22.3 °C
- Aeration: gentle aeration via narrow glass tubes
- Test item concentration/s: 100 mg/L
- Method of administration: direct weighing
- Medium renewal: daily
- Criteria of effects: The criterion of death used in this study was the absence of response to physical stimulation; in addition to observations on mortality at 2, 24, 48, 72 and 96 hours, type and incidence of sub-lethal effects compared with control fish were observed - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- >= 72.061 µg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 72.061 µg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- > 72.061 µg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- All results are expressed in terms of nominal and geometric mean measured concentrations. Effective concentrations ranged from 0.047 % to 0.136 % of nominal values in the freshly prepared medium and from 0.021 % to 0.091 % of nominal values in the medium after 24 hours of exposure.
- Validity criteria fulfilled:
- yes
- Remarks:
- Mortality in the controls < 10 % by the end of the test; Dissolved oxygen concentration >60 % of the air-saturation value throughout the exposure period; pH did not vary by more than 1 unit
- Conclusions:
- No toxic effects against fish were observed at the limit of water solubility under exposure conditions. All results are expressed in terms of nominal and geometric mean measured concentrations. Effective concentrations ranged from 0.047 % to 0.136 % of nominal values in the freshly prepared medium and from 0.021 % to 0.091 % of nominal values in the medium after 24 hours of exposure.
- Executive summary:
According to the method described in the Council Regulation (EC) No 440/2008, Method C.1 'Acute toxicity for Fish' (2008) which is in most parts equivalent to the OECD Guideline for Testing of Chemicals No. 203 'Fish, Acute Toxicity Test' (1992) the acute toxic effects (mortality) of various concentrations of a test item to a freshwater fish species were assessed. A range finding test preceded the main test. It provided information about the range of concentrations which were used in the main test. The following nominal concentrations of the test item were tested in the range-finding test: 10 and 100 mg/L. Because of the anticipated low toxicity of the test item, fish were exposed in the main test to a limit test concentration of nominally 100 mg/L for a period of 96 hours. As no toxic effects against fish were observed, no statistical analysis was required to determine the LC 50. Additionally any abnormal behaviour or appearance of the fish was reported every 24 hours. The main test was conducted as semi-static test with daily renewal of test solutions. During the test a temperature range of 20 - 24 °C was maintained in the test vessels, with a maximum temperature fluctuation of +/- 0.6 °C in the test. The temperature, the pH and the oxygen values were measured at the beginning of the test and every 24 hours thereafter. The temperature, the pH and the oxygen values were measured every day in the freshly prepared media and in the media after 24 hours of exposure. The validity criteria of the test were met. No toxic effects against fish were observed at the limit of water solubility under exposure conditions. All results are expressed in terms of nominal and geometric mean measured concentrations. Effective concentrations ranged from 0.047 % to 0.136 % of nominal values in the freshly prepared medium and from 0.021 % to 0.091 % of nominal values in the medium after 24 hours of exposure. This toxicity study is classified as acceptable and satisfies the guideline requirements for the toxicity study to fish.
Reference
Description of key information
No toxic effects against fish were observed at the limit of water solubility under exposure conditions (for details see also chapter 4.8). All results are expressed in terms of nominal and geometric mean measured concentrations. Effective concentrations ranged from 0.047 % to 0.136 % of nominal values in the freshly prepared medium and from 0.021 % to 0.091 % of nominal values in the medium after 24 hours of exposure (Woelz, 2018).
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 72.061 µg/L
Additional information
"Should read: 96 h-LC50 > 72.061 µg/L"
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