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Diss Factsheets
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EC number: 834-791-6 | CAS number: 1310681-27-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- other information
- Study period:
- 2018-04-20 to 2019-01-31
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Cross-reference
- Reason / purpose for cross-reference:
- read-across source
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2019
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Version / remarks:
- (1992)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- [2,2,6,6-tetramethyl-4-(trimethylazaniumyl)piperidin-1-yl]oxidanyl chloride
- EC Number:
- 814-375-0
- Cas Number:
- 67036-09-3
- Molecular formula:
- C12H26N2OCl
- IUPAC Name:
- [2,2,6,6-tetramethyl-4-(trimethylazaniumyl)piperidin-1-yl]oxidanyl chloride
- Test material form:
- solid
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Activated sludge from a municipal waste water treatment plant in Germany, which predominantely treats domestic sewage. The activated sludge is collected from aeration tank.
- Storage conditions: kept under aerobic conditions between sampling and inoculation.
- Preparation of inoculum for exposure: not pre-adapted or altered prior to testing.
- Concentration of sludge: Concentration of 30 mg/L in the mineral media - Duration of test (contact time):
- 28 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 14 other: mg carbon/L
- Based on:
- ThCO2
- Remarks:
- nominal concentration
- Initial conc.:
- 24 mg/L
- Based on:
- test mat.
- Initial conc.:
- 35.9 other: mg/test vessel
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: mineral salts medium
- Additional substrate: no
- Solubilising agent: no
- Test temperature: 21.95 - 25.78 °C
- pH: 7.4 +/- 0.2
- pH adjusted: yes
- Aeration of dilution water: Vessels will be sealed and aerated with CO2-free air overnight before beginning of the study.
- Suspended solids concentration: 30 mg/L in the test vessels
- Continuous darkness: diffuse light conditions of low intensity.
- Other: Test vessels were permanently stirred by magnetic stirres during study period.
TEST SYSTEM
- Culturing apparatus: Test vessel 2 L glass bottles. Test volume 1500 mL.
- Number of culture flasks/concentration: two replicates per group (Test item group, Reference control group, Inoculum control group), toxicity control group one replicate
- Method used to create aerobic conditions: sparged with CO2-free air.
- Measuring equipment: trap vessel with barium hydroxide
- Test performed in closed vessels: sealed vessels
- Details of trap for CO2 and volatile organics if used: Trap vessels with barium hydroxide. At intervals the first trap was disconnected for titration. The other trap vessels moved up one position.
SAMPLING
- Sampling frequency: CO2 measurment intervals not longer than 5 days. 11 sampling time points during the study period.
- Sampling method: CO2 trap vessels
CONTROL AND BLANK SYSTEM
- Inoculum blank: only inoculum
- Reference control: reference item and inoculum
- Toxicity control: test item, reference item and inoculum
Reference substance
- Reference substance:
- benzoic acid, sodium salt
- Remarks:
- 25 mg/L of the test item/test vessel (50 mg/L, corresponds to 10 mg carbon/L).
Results and discussion
% Degradation
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 0
- Sampling time:
- 28 d
- Details on results:
- The CO2 concentrations in the test vessels were measured at time intervals throughout the 28 day testing period. All treatments were corrected by the mean value of the inoculum controls and the biochemical carbon dioxide yield was calculated. The percent degradation was derived from the ThCO2, divided by the theoretical production.
The test item showed a degradation of 0% while the reference item amounted to a degradation of 115 % after 28 days. In the toxicity control, biodegradation amounted to ≥ 34 % within 14 days. Thus, according to the test guidelines, the test item had no significant inhibitory effect on activated sludge microorganisms at the tested concentration due to a biodegradation > 25 %. Measured pH values were in the range between 7.39 and 8.03.
BOD5 / COD results
- Results with reference substance:
- Sodium benzote: Biodegradation after 28 days: 115%
Any other information on results incl. tables
Percentage of degradation
Degradation1[%] |
|||||
Time [days] |
Test Item |
Reference Control |
ToxicityControl |
||
Replicate 1 |
Replicate 2 |
Replicate 1 |
Replicate 2 |
- |
|
1 |
0 |
0 |
12 |
13 |
6 |
4 |
0 |
0 |
33 |
45 |
18 |
6 |
0 |
0 |
52 |
63 |
25 |
8 |
0 |
0 |
64 |
73 |
27 |
11 |
0 |
0 |
83 |
90 |
34 |
15 |
0 |
0 |
91 |
96 |
37 |
18 |
0 |
0 |
98 |
102 |
39 |
22 |
0 |
0 |
103 |
104 |
40 |
25 |
0 |
0 |
110 |
112 |
43 |
28 |
0 |
0 |
114 |
116 |
44 |
29 |
0 |
0 |
114 |
117 |
45 |
29 |
0 |
0 |
113 |
115 |
44 |
29 |
0 |
0 |
113 |
116 |
44 |
|
|
|
|
||
Mean (28 days) |
0 |
115 |
- |
1Corrected for the mean oxygen uptake of the inoculum controls
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- Validity criteria (replicate variability >20%, reference compound reach 60% after 14 d, inoculum blank <70 mg/L after 28 d, pH at 7.4) were met.
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The ready biodegradability of TMA-TEMPO was assessed with the CO2 Evolution Test according to OECD Guideline 301 B. TMA-TEMPO was tested at a nominal concentration of 14 mg Carbon/L. The following biodegradation was determined at the end of the 28-d period: TMA-TEMPO: 0 % and Sodium benzoate: 115 %. Since the pass value of > 60 % was not reached within 28 days TMA-TEMPO cannot be considered to be readily biodegradable under the tested conditions according to OECD guideline 301 B. TMA-TEMPO had no significant inhibitory effect on activated sludge microorganisms at the tested concentrations.
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