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EC number: 254-754-2 | CAS number: 40027-38-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 23-05-1995
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Non-GLP, No certificate of Analyses included. 30 minutes exposure for a sorbing substance.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- A stock suspension was used of 1 g/L.
The test was performed using the following test concentrations: Control (duplicate), 6.3, 12.5, 25, 50 and 100 mg/L - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Secondary activated sludge was obtained from the WWTP Nieuwgraaf in Duiven. The WWTP Nieuwgraaf is an actvated sludge plant treating predominantly domestic waste water. The activated sludge concentration in the incubation vessels was 1.0 g DW/L.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 30 min
- Hardness:
- Sythetic sewage feed was used: This was made by dissolving 16 peptone, 11 g meat extract, 3 g Urea, 0.7 g NaCl, 0.4 g CaCl2.2H2O, 0.2 g MgSO4.7H2O and 2.8 g K2HPO4 in one liter of deionized water.
- Test temperature:
- 21 °C
- pH:
- pH ranged from 6.7 to 7.3
- Nominal and measured concentrations:
- Control (duplicate), 6.3, 12.5, 25, 50 and 100 mg/L nominal
- Details on test conditions:
- The activated sludge respiration inhibition test was performed according to OECD test guideline 209. In this test the respiration rate of activated sludge fed with a standard amount of synthetic sewage was measured. The respiration rate of the same activated sludge in the presence of various concentrations of the test substance under otherwise identical conditions was also measured.
- Duration:
- 30 min
- Dose descriptor:
- other: EC20
- Effect conc.:
- 10.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 30 min
- Dose descriptor:
- EC50
- Effect conc.:
- 25.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: 95% conf. lim. 24.6 - 25.7
- Duration:
- 30 min
- Dose descriptor:
- other: EC80
- Effect conc.:
- 58.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- The control respiration rates are within 0% of each other
- Reported statistics and error estimates:
- The respiration rate was expressed as a percentage of the mean of two controls of the respiration rate: (1 - 2Rs/(Rc1 + Rc2))*100 = percentage inhibition where: Rs = oxygen consumption rate at tested concentration of the test substance; Rc = oxygen consumption rate of controls.
The EC values were computed from the best fitted lines (least square method) through the points given by the probit of the percentage inhibition and the logarithm of the concentration of the test substance. Confidence limits were computed based on Fieller's Theorem. - Validity criteria fulfilled:
- yes
- Conclusions:
- Activated sludge EC50 (30 min) of Duomeen O = 25.1 mg/L
- Executive summary:
In order to predict effects of chemicals in the environment and in biological waste water treatment plants, an activated sludge respiration inhibition test was performed. The toxicity to activated sludge was determined in accordance with OECD test guideline 209
The toxicity of Duomeen O to activated sludge was determined by measuring the respiration ratee after a contact time of 30 minutes at various concentrations of the test substance. The inhibitory effect of Duomeen O at a perticular concentration was expressed as a percentage of the two controls. From the results ECvalues were calcualted. The EC50 of Duomeen O for activated sludge is 25.1 mg/L with 95% CL of 24.6 and 25.7 mg/L. The EC20 and EC80 are 10.7 and 58.7 mg/L resp.
Reference
Respiration rates of the activated sludge, inhibition percentages, probit values and pH at various concentrations of test substance.
Concentration mg/L |
Activity mg O2/min.L |
Inhibition % |
Probit |
pH |
Control |
0.24 |
- |
6.7 |
|
Control |
0.24 |
- |
6.7 |
|
100.0 |
0.00 |
100 |
8.10 |
7.2 |
50.0 |
0.06 |
75 |
5.67 |
6.8 |
25.0 |
0.12 |
50 |
5.00 |
6.8 |
12.5 |
0.18 |
25 |
4.33 |
6.8 |
6.3 |
0.22 |
8 |
4.59 |
6.8 |
Description of key information
In contrary to studies with soil and sediment where sorption is mainly driven by ionic interaction, is sorption to sludge mainly driven by sorption to organic matter. In soil and sediment both mono- and di-oleates are expected to dissociate completely because through sorption of the cationic groups the dissociation equilibrium will lean strongly to the side of full dissociation but for sorption to organic matter which is mainly based on hydrophobic interaction this dissociation equilibrium lies more to the side of oleyldiamine di-oleates salt. Toxicity to micro-organisms of oleyldiamines and oleyldiamines di-oleates salts are therefore expect to be different. The toxicity of oleyldiamine mono-oleates to sludge bacteria is expected to be somewhere in between. A geometric mean of the two results can unfortunately not be used as the study with oleyldiamine which resulted in an EC50 of 25.1 mg/L is based on 30 minutes exposure and the study with oleyldiamine di-oleates which resulted in an NOEC of 1000 mg/L is based on a 3 hours exposure. As a worst-case the result of the oleyldiamine will be used for read across.
For N-(Oleyl alkyl)- 1,3 -propanediamine di-oleates a 3h NOEC of 1000 mg/L was observed and for N-(Oleyl alkyl)- 1,3 -propanediamine di-oleates are not comparable because the test solutions were prepared differently (bulk approach applied to the di-oleates), toxicity test results from studies with sorbents like soil, sediment and sludge in the terrestrial, benthic and sludge tests should be comparable and should allow read across.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 25.1 mg/L
Additional information
Oleyl diamines di-oleates toxicity to microorganisms
A respiration inhibition test with activated sludge according to OECD Guideline No. 209 was carried out with N-[(9Z)-octadec-9-en-1-yl]propane-1,3-diaminium di[(9Z)-octadec-9-enoate] (oleyldiamine di-oleates).
The test system was activated sludge of the municipal treatment plant of 31137 Hildesheim, Germany. The test was carried out under static conditions with the substance concentrations of 560 - 750 - 1000 mg/L in a geometrical series with a dilution factor of 1.33. Based on the concentration of actiev ingredien, the 3h-NOEC and 3h-EC50 were found to be 1000 and >1000 mg/L respectively (based on active ingredient).
Oleyl diamines toxicity to microorganisms
The lowest EC50 for microorganisms as observed in an OECD 209 test is 25.1 mg/L for N-9-octa-decenyl-, (Z)- propane-1,3-diamine (7173-62-8)
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