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EC number: 701-293-4 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / bone marrow chromosome aberration
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Near-guideline, GLP-compliant study. Adequate for assessment.
- Justification for type of information:
- Read across justification included in Section 13
Cross-reference
- Reason / purpose for cross-reference:
- read-across: supporting information
Reference
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / bone marrow chromosome aberration
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Near-guideline, GLP-compliant study. Adequate for assessment.
- Justification for type of information:
- Read across justification included in Section 13
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 475 (Mammalian Bone Marrow Chromosome Aberration Test)
- Principles of method if other than guideline:
- The frequency of chromosomal aberrations in rat bone marrow was determined following 5 consecutive daily treatments via oral gavage.
- GLP compliance:
- yes
- Type of assay:
- chromosome aberration assay
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Charles River Breeding Laboratories
- Group housed: 4/sex/cage
- Diet (Purina Laboratory Chow) and tap water available ad libitum
- No further details - Route of administration:
- oral: gavage
- Vehicle:
- corn oil
- Details on exposure:
- 5 consecutive daily exposures, 24 hr apart, administered by oral gavage in corn oil
- Duration of treatment / exposure:
- 5 days
- Frequency of treatment:
- Daily
- Post exposure period:
- Animals sacrificed 6 hr after last treatment
- Remarks:
- Doses / Concentrations:
0.1, 0.3 and 1.0 g/Kg bw/d
Basis:
other: nominal in vehicle - No. of animals per sex per dose:
- 0.1 g/Kg/d, 0.3 g/Kg/d, positive controls, vehicle controls: 10 males and 10 females per treatment
1 g/Kg/d: 13 males and 13 females per treatment - Control animals:
- yes, concurrent vehicle
- Positive control(s):
- triethylenemelamine, 1 mg/Kg bw, administered as single i.p. injection
- Tissues and cell types examined:
- Tibial bone marrow cells
- Details of tissue and slide preparation:
- CRITERIA FOR DOSE SELECTION:
Based on results of preliminary dose range findings investigation (survival following 5 consecutive daily gavage treatments of 0, 0.5, 1.5 or 3.0 g/kg bwt)
DETAILS OF SLIDE PREPARATION:
Tibial bone marrow cells washed, isolated (centrifugation) and fixed (methanol:acetic acid); air-dried spreads stained with 5% Giemsa (pH 6.8)
METHOD OF ANALYSIS:
Slides were coded and scored blind. 50 spreads per animal were subject to microscopic examination and scored for gaps, breaks, fragments and reunion figures. At least 500 cells per spread were evaluated. - Evaluation criteria:
- Potential aberrations included: chromatid gap or break; chromosome gap or break; chromatid deletion; fragment or acentric fragment; transolcation; triradial; quadriradial; pulverised chromsome or cells; complex rearrangement; ring chromosome; dicentric chromosome; minute chromsome; greater than 10 aberrations; polyploid; hyperploid
- Statistics:
- Students T-test
- Sex:
- male/female
- Genotoxicity:
- negative
- Toxicity:
- yes
- Remarks:
- 40-60% mortality at 1.5 g/Kg bw; 30-100% mortality at 3 g/Kg bw
- Vehicle controls validity:
- valid
- Negative controls validity:
- not examined
- Positive controls validity:
- valid
- Conclusions:
- Interpretation of results (migrated information): negative
Not clastogenic to bone marrow in vivo. - Executive summary:
The clastogenic potential of catalytic cracked clarified oil towards rat bone marrow cells was invested in vivo in a near-guideline GLP-compliant investigation. Animals received 5 consecutive daily gavage treatments of 0 (corn oil), 0.1, 0.3 or 1.0 g/Kg bw/d prior to sacrifice, while positive controls were given a single i.p. injection of 0 or 1 mg/Kg triethylenemelamine. At least 500 cells from 50 Giemsa stained spreads per animal were examined microscopically and scored blind for the presence of structural and numerical aberrations.
There was no treatment-related alteration in chromosomal aberrations however a satisfactory response was obtained in the positive control group. The test substance was not clastogenic in this in vivo test.
The test substance did not significantly increase the frequency of chromosomal aberrations, nor did it increase the mitotic index in male and female bone marrow cells at any dose.
The positive control group animals produced significant increases in chromosomal aberration frequencies.
Condition |
Aberrations per cell |
Percent cells with structural aberrations |
Mitotic index % |
||
Structural |
Numerical |
1 or more |
2 or more |
||
Negative control (corn oil) |
0.002 (m) 0.007 (f) |
0.006 (m) 0.002 (f) |
0.6 (m) 0.2 (f) |
0 (m) 0 (f) |
5.5 (m) 4.5 (f) |
Positive control (TEM) |
0.00 |
>4.8 (m) >2.5 (f) |
69.7 (m) 43.9 (f) |
62.6 (m) 32.8 (f) |
0.8 (m) 1.0 (f) |
0.1 g/Kg bw |
0.002 (m) 0.007 (f) |
0.006 (m) 0.016 (f) |
0.2 (m) 0.7 (f) |
0 (m) 0 (f) |
5.4 (m) 4.1 (f) |
0.3 g/Kg bw |
0.004 (m) 0.008 (f) |
0.014 (m) 0.008 (f) |
0.4 (m) 0.8 (f) |
0 (m) 0 (f) |
5.0 (m) 4.0 (f) |
1.0 g/Kg bw |
0.004 (m) 0.010 (f) |
0.002 (m) 0.004 (f) |
0.4 (m) 1.0 (f) |
0 (m) 0 (f) |
6.0 (m) 3.3 (f) |
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 985
- Report date:
- 1985
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 475 (Mammalian Bone Marrow Chromosome Aberration Test)
- Principles of method if other than guideline:
- The frequency of chromosomal aberrations in rat bone marrow was determined following 5 consecutive daily treatments via oral gavage.
- GLP compliance:
- yes
- Type of assay:
- chromosome aberration assay
Test material
- Reference substance name:
- 64741-62-4
- Cas Number:
- 64741-62-4
- IUPAC Name:
- 64741-62-4
- Test material form:
- other: Viscous hydrocarbon liquid
- Details on test material:
- Catalytic cracked clarified oil (CCCO), API 81-15, CAS No.
64741-62-4.
Dark brown viscous liquid
Data below taken from American Petroleum Institute (1985d). In-vivo sister chromatid exchange (SCE) assay. Catalytic cracked clarified oil, API Sample 81-15, CAS No. 64741-62-4. Testing laboratory: Microbiological Associates Inc., 5221 River Road, Bethesda, MD 20816, USA. Owner company: American Petroleum Institute, 2101 L Street, Northwest, Washington, DC 20037, USA. Study number: 32-32754. Report date: 1985-11-25.
Gravity API: 0.1
Specific gravity: 1.0753
Viscosity in SUS @ 210 °F: 56.1
Flash Point °F: 396
Sulfur wt %: 1.1
Pour Pt °F: 35
Asphaltenes % (MN 596): 4.2
Carbon residue wt %: 4.6
Ash wt %: 0.05
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Charles River Breeding Laboratories
- Group housed: 4/sex/cage
- Diet (Purina Laboratory Chow) and tap water available ad libitum
- No further details
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- corn oil
- Details on exposure:
- 5 consecutive daily exposures, 24 hr apart, administered by oral gavage in corn oil
- Duration of treatment / exposure:
- 5 days
- Frequency of treatment:
- Daily
- Post exposure period:
- Animals sacrificed 6 hr after last treatment
Doses / concentrations
- Remarks:
- Doses / Concentrations:
0.1, 0.3 and 1.0 g/Kg bw/d
Basis:
other: nominal in vehicle
- No. of animals per sex per dose:
- 0.1 g/Kg/d, 0.3 g/Kg/d, positive controls, vehicle controls: 10 males and 10 females per treatment
1 g/Kg/d: 13 males and 13 females per treatment - Control animals:
- yes, concurrent vehicle
- Positive control(s):
- triethylenemelamine, 1 mg/Kg bw, administered as single i.p. injection
Examinations
- Tissues and cell types examined:
- Tibial bone marrow cells
- Details of tissue and slide preparation:
- CRITERIA FOR DOSE SELECTION:
Based on results of preliminary dose range findings investigation (survival following 5 consecutive daily gavage treatments of 0, 0.5, 1.5 or 3.0 g/kg bwt)
DETAILS OF SLIDE PREPARATION:
Tibial bone marrow cells washed, isolated (centrifugation) and fixed (methanol:acetic acid); air-dried spreads stained with 5% Giemsa (pH 6.8)
METHOD OF ANALYSIS:
Slides were coded and scored blind. 50 spreads per animal were subject to microscopic examination and scored for gaps, breaks, fragments and reunion figures. At least 500 cells per spread were evaluated. - Evaluation criteria:
- Potential aberrations included: chromatid gap or break; chromosome gap or break; chromatid deletion; fragment or acentric fragment; transolcation; triradial; quadriradial; pulverised chromsome or cells; complex rearrangement; ring chromosome; dicentric chromosome; minute chromsome; greater than 10 aberrations; polyploid; hyperploid
- Statistics:
- Students T-test
Results and discussion
Test results
- Sex:
- male/female
- Genotoxicity:
- negative
- Toxicity:
- yes
- Remarks:
- 40-60% mortality at 1.5 g/Kg bw; 30-100% mortality at 3 g/Kg bw
- Vehicle controls validity:
- valid
- Negative controls validity:
- not examined
- Positive controls validity:
- valid
Any other information on results incl. tables
The test substance did not significantly increase the frequency of chromosomal aberrations, nor did it increase the mitotic index in male and female bone marrow cells at any dose.
The positive control group animals produced significant increases in chromosomal aberration frequencies.
Condition |
Aberrations per cell |
Percent cells with structural aberrations |
Mitotic index % |
||
Structural |
Numerical |
1 or more |
2 or more |
||
Negative control (corn oil) |
0.002 (m) 0.007 (f) |
0.006 (m) 0.002 (f) |
0.6 (m) 0.2 (f) |
0 (m) 0 (f) |
5.5 (m) 4.5 (f) |
Positive control (TEM) |
0.00 |
>4.8 (m) >2.5 (f) |
69.7 (m) 43.9 (f) |
62.6 (m) 32.8 (f) |
0.8 (m) 1.0 (f) |
0.1 g/Kg bw |
0.002 (m) 0.007 (f) |
0.006 (m) 0.016 (f) |
0.2 (m) 0.7 (f) |
0 (m) 0 (f) |
5.4 (m) 4.1 (f) |
0.3 g/Kg bw |
0.004 (m) 0.008 (f) |
0.014 (m) 0.008 (f) |
0.4 (m) 0.8 (f) |
0 (m) 0 (f) |
5.0 (m) 4.0 (f) |
1.0 g/Kg bw |
0.004 (m) 0.010 (f) |
0.002 (m) 0.004 (f) |
0.4 (m) 1.0 (f) |
0 (m) 0 (f) |
6.0 (m) 3.3 (f) |
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information): negative
Not clastogenic to bone marrow in vivo. - Executive summary:
The clastogenic potential of catalytic cracked clarified oil towards rat bone marrow cells was invested in vivo in a near-guideline GLP-compliant investigation. Animals received 5 consecutive daily gavage treatments of 0 (corn oil), 0.1, 0.3 or 1.0 g/Kg bw/d prior to sacrifice, while positive controls were given a single i.p. injection of 0 or 1 mg/Kg triethylenemelamine. At least 500 cells from 50 Giemsa stained spreads per animal were examined microscopically and scored blind for the presence of structural and numerical aberrations.
There was no treatment-related alteration in chromosomal aberrations however a satisfactory response was obtained in the positive control group. The test substance was not clastogenic in this in vivo test.
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