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EC number: 237-377-8 | CAS number: 13767-32-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 11 October 2018 - 19 December 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- The concentration of the test item were measured at each test concentration at the beginning and end of the test in triple determination.
The samples were preserved with acid (HNO3, 65 %) and cool storage. - Vehicle:
- no
- Details on test solutions:
- A stock solution of the test item of 32 mg/l was prepared in dilution water. The solution was stirred overnight at room temperature afterwards. The next day the stock solution was filtered, so that only the dissolved proportion was tested. The test solutions were prepared by adding dilution water (ISO Test water) to the stock solution of the test item.
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Strain/clone: Straus
- Source: In-house culture
- Age of parental stock (mean and range, SD): Less than 24 hours old.
- Feeding during test: No.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Test temperature:
- 19.5-20.5 °C
- pH:
- 7.47-7.87
- Dissolved oxygen:
- 8.8-8.9 mg/l
- Nominal and measured concentrations:
- Nominal concentrations: 0, 1, 2, 4, 8, 16, 32 mg/L
Measured concentrations (initial): 0.907, 1.90, 3.68, 7.69, 15.1, 30.5 mg/L
Measured concentrations (final): 0.813, 1.79, 3.45, 7.39, 14.9, 29.5 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 50 mL glass beakers
- Type (delete if not applicable): coveed with watch glass dishes
- Material, size, headspace, fill volume: glass, 50 mL, - , 20 mL
- Aeration: No.
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: ISO Test water
- Culture medium different from test medium: No.
OTHER TEST CONDITIONS
- Adjustment of pH: No.
- Photoperiod: Dark.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Mobility
RANGE-FINDING STUDY
- Test concentrations: 0, 0.16, 0.8, 4.0, 20, 100 mg/L (static, 48 hours).
- Results used to determine the conditions for the definitive study: 0, 0, 10, 90 and 100 % immobilization. The expected EC50-value (48 hours) was between 4 and 20 mg/L: - Reference substance (positive control):
- yes
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities: No.
- Mortality of control: No immobilization was observed in control.
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: No.
- Effect concentrations exceeding solubility of substance in test medium: No. - Results with reference substance (positive control):
- The reliability of the test conditions was tested with the reference item (positive control) potassium dichromate (K2Cr2O7) in a separate study (R18-1001-008) at 25/26.07.2018. The determined EC50-24h of 1.9 mg/l lies within the range from the inter laboratory test to ISO 6341. So the test conditions were reliable.
- Reported statistics and error estimates:
- From the determined effects the EC50-, EC20- and EC10-values of the test item after 24 and 48 hours were calculated by non-linear regression. In addition, the NOEC/LOEC of the test item were determined. In both cases the calculation was carried out with the software SigmaPlot 13.
- Validity criteria fulfilled:
- yes
- Remarks:
- (in the control <10% of immobilization observed, dissolved oxygen concentration at the end of the test >3mg/L)
- Conclusions:
- The EC50 was determined to be 8.0 mg/L after 48 hours in Daphnia magna.
- Executive summary:
An acute Daphnia immobilization test was performed according to the OECD Guideline 202 (GLP study). Based on preliminary tests, 20 Daphnia magna per dose were (in 4 replicates) were exposed to 0 (control), 1, 2, 4, 8, 16 and 32 mg/L test item for 48 hours under static conditions. The recovery of the test item was normally between 81 and 96 %. In the measurement the recovery was within the range of ± 20 % of the nominal concentration. Thus it can be assumed that the test item was stable over the test duration. Because the difference between the nominal and the measured concentration was smaller than 20 %, the results below were calculated with the nominal concentrations at the beginning of the test. No immobilised daphnids were observed in the control group. The dissolved oxygen concentration at the end of the test was ≥ 8 mg/l in each test concentration. The observed percentage of immobilization at the end of the test was 0% (control), 0% (1 mg/L), 0% (2 mg/L), 0% (4 m/L), 55% (8 mg/L), 85% (16 mg/L) and 95% (32 mg/L). The 48h-EC50 of the test item was found to be 8.0 mg/L.
Reference
Nominal and measured concentrations:
Nominal (mg/L) |
Correspondingdetermined |
Average of the |
||
at 0 hours (mg/L) |
at 48 hours (mg/L) |
at 0 hours (mg/L) |
at 48 hours (mg/L) |
|
0 |
<0.24 |
<0.24 |
<0,07 |
<0,07 |
1 |
0.907 |
0.813 |
0.263 |
0.236 |
2 |
1.90 |
1.79 |
0.551 |
0.521 |
4 |
3.68 |
3.45 |
1.068 |
1.001 |
8 |
7.69 |
7.39 |
2.231 |
2.144 |
16 |
15.1 |
14.9 |
4.387 |
4.330 |
32 |
30.5 |
29.5 |
8.847 |
8.559 |
Recovery of the nominal concentrations:
Nominal (mg/L) |
Recoveryof ZnMoO4 |
|
at 0 hours (%) |
at 48 hours (%) |
|
1 |
90.7 |
81.3 |
2 |
94.9 |
89.7 |
4 |
92.1 |
86.3 |
8 |
96.1 |
92.4 |
16 |
94.5 |
93.3 |
32 |
95.3 |
92.2 |
The recovery of the test item was normally between 81 and 96 %. In the measurement the recovery was within the range of ± 20 % of the nominal concentration. Thus it can be assumed that the test item was stable over the test duration. Because the difference between the nominal and the measured concentration was smaller than 20 %, the results below were calculated with the nominal concentrations at the beginning of the test.
Test results:
Test concentration (mg/L) |
Number of daphnids |
Number of immobilised |
Percentage immobilised |
Percentage immobilised |
|||
24 h |
48 h |
24 h |
48 h |
24 h |
48 h |
||
0 |
5 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
5 |
0 |
0 |
0 |
0 |
||
0 |
5 |
0 |
0 |
0 |
0 |
||
0 |
5 |
0 |
0 |
0 |
0 |
||
1 |
5 |
0 |
0 |
0 |
0 |
0 |
0 |
1 |
5 |
0 |
0 |
0 |
0 |
||
1 |
5 |
0 |
0 |
0 |
0 |
||
1 |
5 |
0 |
0 |
0 |
0 |
||
2 |
5 |
0 |
0 |
0 |
0 |
0 |
0 |
2 |
5 |
0 |
0 |
0 |
0 |
||
2 |
5 |
0 |
0 |
0 |
0 |
||
2 |
5 |
0 |
0 |
0 |
0 |
||
4 |
5 |
0 |
0 |
0 |
0 |
0 |
0 |
4 |
5 |
0 |
0 |
0 |
0 |
||
4 |
5 |
0 |
0 |
0 |
0 |
||
4 |
5 |
0 |
0 |
0 |
0 |
||
8 |
5 |
0 |
3 |
0 |
60 |
10 |
55 |
8 |
5 |
1 |
2 |
20 |
40 |
||
8 |
5 |
0 |
3 |
0 |
60 |
||
8 |
5 |
1 |
3 |
20 |
60 |
||
16 |
5 |
0 |
4 |
0 |
80 |
25 |
85 |
16 |
5 |
1 |
3 |
20 |
60 |
||
16 |
5 |
2 |
5 |
40 |
100 |
||
16 |
5 |
2 |
5 |
40 |
100 |
||
32 |
5 |
1 |
5 |
20 |
100 |
25 |
95 |
32 |
5 |
2 |
5 |
40 |
100 |
||
32 |
5 |
1 |
5 |
20 |
100 |
||
32 |
5 |
1 |
4 |
20 |
80 |
Calculated effect concentrations after 24 and 48 hours:
|
Results after 24 hours |
Results after 48 hours |
||||
Concentration (mg/L) |
95% Conf. Limit |
Concentration (mg/L) |
95% Conf. Limit |
|||
Lower |
Upper |
Lower |
Uppper |
|||
EC10 |
8.3 |
3.6 |
14.5 |
4.3 |
3.4 |
5.5 |
EC20 |
19 |
12.4 |
>32 |
5.4 |
4.4 |
6.4 |
EC50 |
>32 |
- |
- |
8.0 |
7.1 |
8.9 |
NOEC |
16 |
- |
- |
4 |
- |
- |
LOEC |
32 |
- |
- |
8 |
- |
- |
pH and dissolved oxygen at the beginning and at the end of the test:
Test |
pH-value |
O2-conc. (mg/L) |
||
0 h |
48 h |
0 h |
48 h |
|
0 |
7.87 |
7.85 |
8.8 |
8.9 |
1 |
7.78 |
7.76 |
8.8 |
8.9 |
2 |
7.73 |
7.75 |
8.9 |
8.9 |
4 |
7.67 |
7.73 |
8.9 |
8.9 |
8 |
7.61 |
7.69 |
8.9 |
8.9 |
16 |
7.58 |
7.66 |
8.9 |
8.9 |
32 |
7.47 |
7.66 |
8.8 |
8.8 |
Description of key information
Short-term toxicity to aquatic invertebrates: Key study: Test method OECD 202. GLP study. The EC50 was determined to be 8.0 mg/L after 48 hours in Daphnia magna.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 8 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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