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EC number: 272-823-5 | CAS number: 68916-18-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 21 May to 21 June 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- yes
- Remarks:
- no impact on study validity
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: CF02
- Expiration date of the lot/batch: 31.01.2019
- Purity test date: not available
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: stored cold at 4°C in the dark
- Stability under test conditions: stable
- Solubility and stability of the test substance in the solvent/vehicle: water solubility 2.85 mg/L
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium: not available
TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: Due to the low aqueous solubility and complex nature of the test item, for the purposes of the test, the test medium was prepared as a Water Accommodated Fraction (WAF). A 23-hour stirring period followed by a 1-hour standing period was considered sufficient to ensure equilibration between the test item and aqueous phase.
- Preliminary purification step (if any): none
- Final dilution of a dissolved solid, stock liquid or gel: none
- Final preparation of a solid: not applicable
OTHER SPECIFICS: none - Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: control and 100 mg/L loading rate WAF test group
- Sampling method: not available
- Sample storage conditions before analysis: not available - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Due to the low aqueous solubility and complex nature of the test item, the test medium was prepared as a Water Accommodated Fraction (WAF).
- Eluate: not specified
- Differential loading: In the range-finding test Daphnia magna were exposed to a series of nominal loading rates of 1.0, 10 and 100 mg/L. Based on the results of the range-finding test a "limit test" was conducted at a loading rate of 100 mg/L.
- Controls: control group: reconstituted water (Elendt M7), positive control: potassium dichromate at concentrations of 0.32, 0.56, 1.0, 1.8 and 3.2 mg/L.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): none
- Concentration of vehicle in test medium (stock solution and final test solution(s) or suspension(s) including control(s)): not applicable
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): none - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Strain/clone: not specified
- Justification for species other than prescribed by test guideline: not applicable
- Source: in-house laboratory cultures
- Age of parental stock (mean and range, SD): not available
- Feeding during test: no
- Food type: not applicable
- Amount: not applicable
- Frequency: not applicable
ACCLIMATION
- Acclimation period: not applicable
- Acclimation conditions (same as test or not): not applicable
- Type and amount of food: not applicable
- Feeding frequency: not applicable
- Health during acclimation (any mortality observed): not applicable
QUARANTINE (wild caught)
- Duration: none
- Health/mortality: none - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Test temperature:
- 21- 22°C
- pH:
- 7.9 ± 0.3
- Dissolved oxygen:
- = 3 mg/L
- Nominal and measured concentrations:
- Range finding test: 1.0, 10, 100 mg/L nominal loading rate
Definitive test: 100 mg/L nominal loading rate - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass beakers
- Material, size, headspace, fill volume: glass, 150 mL
- Aeration: none
- Type of flow-through (e.g. peristaltic or proportional diluter): none
- Renewal rate of test solution (frequency/flow rate): none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): none
- Biomass loading rate: not available
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water (Elendt M7)
- Total organic carbon: 18.53C/L at 0 hours
- Particulate matter: none
- Metals: not available
- Pesticides: not available
- Chlorine: not available
- Alkalinity: 7.9 ± 0.3
- Ca/Mg ratio: not available
- Conductivity: not available
- Salinity: not available
- Culture medium different from test medium: not applicable
- Intervals of water quality measurement: not applicable
OTHER TEST CONDITIONS
- Adjustment of pH: none
- Photoperiod: 16 hours light and 8 hours darkness cycle with 20-minute dawn and dusk transition periods
- Light intensity: not specified
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): any immobilization or adverse reactions to exposure were recorded at 24 and 48 hours after the start of exposure.
VEHICLE CONTROL PERFORMED: no
RANGE-FINDING STUDY
- Test concentrations: 1.0, 10 and 100 mg/L nominal loading rate
- Results used to determine the conditions for the definitive study: cumulative immobilization and sub-lethal effects - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Key result
- Duration:
- 48 h
- Dose descriptor:
- other: NOEL
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: WAF
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EL50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: WAF
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities: in the range-finding test in one of the control vessels two of the five Daphnia were immobilized. No sub-lethal effects of exposure were observed throughout the test.
- Observations on body length and weight: not applicable
- Other biological observations: none
- Mortality of control: none
- Other adverse effects control: none
- Abnormal responses: none
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: none
- Effect concentrations exceeding solubility of substance in test medium: none - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: NOEC 0.56 mg/L at 24 and 48 hours, LOEC 1 mg/L at 24 and 48 hours
- Limit test: not applicable
- Dose-response test: not available
- ECx: EC50 0.79 at 24 and 48 hours
- Other: none - Reported statistics and error estimates:
- The 24 and 48-Hour EL50 values were determined by visual inspection of the data. All statistical analysis was carried out using the ToxRat Professional computer software package
- Validity criteria fulfilled:
- yes
- Conclusions:
- In an acute immobilization test performed on Daphnia magna, and conducted according to OECD test guideline 202 and to GLP, the No Observed Effect Level value was determined at 100 mg/L loading rate as a Water Accommodated Fraction (WAF) after 24 and 48 hours of exposure to Coffee, bean, roasted, ext. The effective loading causing 50% response (EL50) was determined at >100 mg/L loading rate WAF.
- Executive summary:
The acute immobilization test conducted according to OECD test guideline 202 and to GLP was performed to assess the acute toxicity of Coffee, bean, roasted, ext. in Daphnia magna.
Due to the low aqueous solubility and complex nature of the test item, the test medium was prepared as a Water Accommodated Fraction (WAF).
Following a preliminary range-finding test, 20 daphnids (4 replicates of 5 animals) were exposed to a WAF of the test item at a nominal loading rate of 100 mg/L for 48 hours at a temperature of 21°C to 22°C under static test conditions. Immobilization and any adverse reactions to exposure were recorded after 24 and 48 hours.
Total Organic Carbon (TOC) analysis of the 100 mg/L loading rate WAF test preparation were performed at 0 and 48 hours. A measured concentration of 18.53 mg C/L was obtained at 0 hours whilst 11.53 mg C/L was obtained at 48 hours.
There was no immobilization in 20 daphnids exposed to a 100 mg/L loading rate WAF for a period of 48 hours and no sub-lethal effects were observed during the study period.
The No Observed Effect Level after 24 and 48 hours of exposure was determined at 100 mg/L loading rate WAF. In addition, the effective loading causing 50% response (EL50) was determined at >100 mg/L loading rate WAF.
Reference
See attached additional information on results.
Description of key information
NOEL: 100 mg/L loading rate WAF (24 and 48h)
EL50: >100 mg/L loading rate WAF (24 and 48h)
Key value for chemical safety assessment
Additional information
The acute immobilization test conducted according to OECD test guideline 202 and to GLP was performed to assess the acute toxicity of Coffee, bean, roasted, ext. in Daphnia magna.
Due to the low aqueous solubility and complex nature of the test item, the test medium was prepared as a Water Accommodated Fraction (WAF).
Following a preliminary range-finding test, 20 daphnids (4 replicates of 5 animals) were exposed to a WAF of the test item at a nominal loading rate of 100 mg/L for 48 hours at a temperature of 21°C to 22°C under static test conditions. Immobilization and any adverse reactions to exposure were recorded after 24 and 48 hours.
Total Organic Carbon (TOC) analysis of the 100 mg/L loading rate WAF test preparation were performed at 0 and 48 hours. A measured concentration of 18.53 mg C/L was obtained at 0 hours whilst 11.53 mg C/L was obtained at 48 hours.
There was no immobilization in 20 daphnids exposed to a 100 mg/L loading rate WAF for a period of 48 hours and no sub-lethal effects were observed during the study period.
The NOEL after 24 and 48 hours of exposure was determined at 100 mg/L loading rate WAF. In addition, the effective loading causing 50% response (EL50) was determined at >100 mg/L loading rate WAF.
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