Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 654-333-7 | CAS number: 5471-90-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2019-2020
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Version / remarks:
- Adopted July 17, 1992
- Deviations:
- no
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Effluent freshly obtained from a municipal sewage treatment plant: 'Waterschap Aa en Maas', 's-Hertogenbosch, The Netherlands, receiving predominantly domestic sewage
- Laboratory culture: /
- Method of cultivation: /
- Storage conditions: The filtrate was kept aerated until inoculation.
- Storage length: /
- Preparation of inoculum for exposure: /
- Pretreatment: /
- Concentration of sludge: 4 mL filtrate of secondary effluent per litre of final volume.
- Initial cell/biomass concentration: /
- Water filtered: yes
- Type and size of filter used, if any: Secondary effluent was filtered through a coarse filter paper, the first 200 mL were discarded. - Duration of test (contact time):
- 28 d
- Initial conc.:
- 21 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- Test duration 28 days for the inoculum blank and test suspension
14 days for the procedure and toxicity control
Test bottles 2 litre brown coloured glass bottles
Milli-RO water Tap-water purified by reverse osmosis (Millipore Corp., Bedford, Mass., USA).
Stock solutions of mineral components
A) 8.50 g KH2PO4
21.75 g K2HPO4
67.20 g Na2HPO4.12H2O
0.50 g NH4Cl
dissolved in 1 litre Milli-RO water, pH 7.4 + 0.2
B) 22.50 g MgSO4.7H2O dissolved in 1 litre Milli-RO water.
C) 36.40 g CaCl2.2H2O dissolved in 1 litre Milli-RO water.
D) 0.25 g FeCl3.6H2O dissolved in 1 litre Milli-RO water.
Mineral medium 1 mL aliquots of solution (A) to (D) were mixed and made up to 1 litre with Milli-RO water. T
Barium hydroxide 0.0125 M Ba(OH)2 (Boom, Meppel, The Netherlands), stored in a sealed vessel to prevent absorption of CO2 from air.
Pre-incubation medium Before the start of the test (Day -1) mineral components, Milli- RO water (ca. 80 % of final volume) and inoculum were added to each bottle. This mixture was aerated with synthetic air overnight to purge the system of CO2.
Type and number of bottles Test suspension: containing test item and inoculum (2 bottles).
Inoculum blank: containing only inoculum (2 bottles)
Procedural control: containing procedural control item and inoculum (1 bottle).
Toxicity control: containing test item, procedural control item and inoculum (1 bottle).
Illumination The test bottles were protected from light.
Preparation At the start of the test (Day 0), test and procedural control item were added to bottles containing microbial organisms and mineral components.
Volumes were made up to 2 L with Milli- RO water, resulting in the mineral medium described before.
Three CO2-absorbers (bottles filled with 100 mL 0.0125 M Ba(OH)2) were connected in series to the exit aeration line of each test bottle. - Reference substance:
- other: sodium acetate
- Test performance:
- The pH values stayed between 7.4 and 7.8.
The temperature recorded in a vessel with Milli-RO water in the same room varied between 22 and 23°C, and complied with the requirements as laid down in the study plan. - Parameter:
- % degradation (CO2 evolution)
- Value:
- 23
- Sampling time:
- 21 d
- Details on results:
- Relative biodegradation values calculated from measurements performed during the test period revealed 23 % biodegradation of CH03951 (based on ThCO2) in both vessels. Thus, the criterion for ready biodegradability (at least 60 % biodegradation within a 10-day window) was not met.
In the toxicity control, 59 % biodegradation was observed. Therefore, CH03951 was found not to inhibit microbial activity at a concentration of 11 mg/L.
Functioning of the test system was checked by testing the procedural control item sodium acetate, which showed a normal biodegradation curve - Results with reference substance:
- Procedural control item was biodegraded by at least 60 % (actual result: 75 % in) within 14 days.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- In conclusion, CH03951 was designated as not readily biodegradable.
- Executive summary:
The objectiveofthe studywas to evaluate test item CH03951 for its ready biodegradability in an aerobic aqueous medium with microbial activity introduced by inoculation with activated sludge.
Procedures described in this report were in compliance with OECD guideline No. 301 B, 1992. In addition, procedures were designed to meet test methods of ISO standard 10634, 2018.
In total, two experiments were performed. In the first experiment a potentially inhibitory effect was found in the toxicity control. Therefore, a second experiment was performed at a 50 % decreased concentration.
CH03951 was an off-white powderwith a purity of approximately 96.85 w/w%. Test item was tested in duplicate at a concentration of 11 mg/L, corresponding to 6 mg TOC/L. Organic carbon content was based on the molecular formula. Theoretical CO2 production (ThCO2) of CH03951 was calculated to be 2.12 mg CO2/mg.
The study consisted of six bottles:
· 2 inoculum blanks (no test item),
· 2 test bottles (CH03951),
· 1 procedural control (sodium acetate) and
· 1 toxicity control (CH03951 plus sodium acetate).
Since CH03951 was not sufficiently soluble to allow preparation of an aqueous stock solution at a concentration of 1 g/L, weighed amounts were added to 2 L test bottles containing medium with microbial organisms and mineral components. To this end, approximately 10 mL of Milli- RO water was added to each weighing bottle containing test item. After vigorous mixing (vortex) the resulting suspension was added quantitatively to the test medium. Test solutions were continuously stirred during the test, to ensure optimal contact between test item and test organisms. Furthermore, test medium was swirled around daily, since the test item tended to float on the water surface.Test duration was28 days for inoculum blank and test item (last CO2measurement on Day 29) and 14 days for procedural and toxicity control (last CO2measurement on Day 15).
Relative biodegradation values calculated from measurements performed during the test period revealed 23 % biodegradation of CH03951 (based on ThCO2) in both vessels. Thus, the criterion for ready biodegradability (at least 60 % biodegradation within a 10-day window) was not met.
In the toxicity control, CH03951 was found not to inhibit microbial activity at a concentration of 11 mg/L.
Since all criteria for acceptability of the test were met, this study was considered to be valid.
In conclusion,CH03951 was designated as not readily biodegradable.
Reference
Description of key information
CH03951 was found to not be readily biodegradable in the OECD 301B test (CO2 evolution test).
Key value for chemical safety assessment
- Biodegradation in water:
- not biodegradable
- Type of water:
- freshwater
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.