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EC number: 218-259-5 | CAS number: 2095-06-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: inherent biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 05 March 2013 - 02 April 2013
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: This study has been performed according to OECD and/or EC guidelines and according to GLP principles.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 013
- Report date:
- 2013
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.9 (Biodegradation: Zahn-Wellens Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: ISO 9888
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- m-(2,3-epoxypropoxy)-N,N-bis(2,3-epoxypropyl)aniline
- EC Number:
- 275-662-9
- EC Name:
- m-(2,3-epoxypropoxy)-N,N-bis(2,3-epoxypropyl)aniline
- Cas Number:
- 71604-74-5
- Molecular formula:
- C15H19NO4
- IUPAC Name:
- 3-(oxiran-2-ylmethoxy)-N,N-bis(oxiran-2-ylmethyl)aniline
- Test material form:
- liquid: viscous
- Details on test material:
- - Description : Clear slightly yellow very viscous liquid
- Test substance storage : In refrigerator (2-8°C) in the dark
- Stability under storage conditions : Stable
- Molecular formula : C15H19NO4
- Molecular weight : 277.3
- CAS Number : 71604-74-5
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source : The source of test organisms was activated sludge freshly obtained from a municipal sewage treatment plant: 'Waterschap Aa en Maas', Heeswijk-Dinther, The Netherlands, receiving predominantly domestic sewage.
- Preparation of the sludge : The sludge was coarsely sieved and washed with mineral medium. The batch of sludge was used within 6 hours of sampling and aerated until required. A small amount of the sludge was weighed and dried at ca. 105°C. From this result the amount of suspended solids (SS) was calculated and adjusted to 3.0 g/l. - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 224.3 mg/L
- Based on:
- DOC
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- TEST CONDITIONS
- Composition of medium: 1 litre mineral medium contains: 10 ml of solution (A), 1 ml of solutions (B) to (D) and Milli-RO water.
Stock solutions of mineral components
A) 8.50 g KH2PO4; 21.75 g K2HPO4; 67.20 g Na2HPO4.12H2O; 0.50 g NH4Cl; dissolved in Milli-Q water and made up to 1 litre, pH 7.4 ± 0.2
B) 22.50 g MgSO4.7H2O dissolved in Milli-Q water and made up to 1 litre.
C) 36.40 g CaCl2.2H2O dissolved in Milli-Q water and made up to 1 litre.
D) 0.25 g FeCl3.6H2O dissolved in Milli-Q water and made up to 1 litre.
- Test temperature: between 20 and 22°C.
- pH: between 6.5 and 7.9, except for the reference substance on day 7, 10 and 17. On day 7 the pH was 8.2 (adjusted to 7.5), on day 10 the pH was 8.2 (adjusted to 7.3) and on day 17 the pH was 6.3 (adjusted to 7.1).
- Oxygen concentrations: between 7.4 and 9.2 mg/l.
- Aeration of dilution water: the test is aerated continuoisly.
- Suspended solids concentration in the test mixtures: 0.75 g SS/l.
- The ratio of inoculum and m-(2,3-epoxypropoxy)-N,N-bis(2,3-epoxypropyl)aniline or reference substance (as DOC) was 3:1.
- Continuous darkness: yes, except during sampling and checking of the test system.
TEST SYSTEM
- Culturing apparatus: 2 litre cylindrical glass vessels.
- Number of culture flasks/concentration: 1
- Method used to create aerobic conditions: aeration with humidifed air.
- Measuring equipment: Shimadzu TOC-VCPH total organic carbon analyzer combined with a Shimadzu ASI-V autosampler (Shimadzu, Kyoto, Japan).
- Test performed in open system.
SAMPLING
- Sampling frequency: At the start of the test (0 h) and 3h after addition of the test substance in order to estimate any adsorption of m-(2,3-epoxypropoxy)-N,N-bis(2,3-epoxypropyl)aniline by the activated sludge; and on day 1, 3, 7, 14, 21, 27 and 28.
- Sampling method: Prior to sampling the volume of the vessels was checked for evaporation and if necessary Milli-Q water was added. The medium was mixed thoroughly and a sample of 100 ml was taken. This sample was passed through a rough paper filter (S&S 604), and thereafter through a 0.45 µm filter (S&S FP 030/0.45). At least, the first 5 ml was discarded. 40 ml was used for DOC analysis. Another 40 ml was stored in a deep-freeze for possible analysis until delivery of the final report with a maximum of three months.
- Sample storage before analysis: Not applicable.
CONTROL AND BLANK SYSTEM
- Inoculum blank: 1
- Reference substance (aniline): 1
Reference substance
- Reference substance:
- aniline
Results and discussion
- Preliminary study:
- Preliminary information: In Project 496991, 203278/A, the flask method applied for the determination of the water solubility of m-(2,3-epoxypropoxy)-N,N-bis(2,3-epoxypropyl)aniline. The water solubility of the test substance at 20°C was 1.78 g/l. The pH of the aqueous samples was 7.5. The preparation method for the Zahn-Wellens test was comparable to the preparation method in the water solubility test.
- Test performance:
- Preparation of test vessels:
1) Test substance mixture (1 vessel):
500 ml mineral medium
500 ml activated sludge (final concentration in test mixture: 0.75 g SS/l)
330 ml of the 10 g/l m-(2,3-epoxypropoxy)-N,N-bis(2,3-epoxypropyl)aniline filtered stock solution.
Made up to 2 litres with mineral medium. DOC of the m-(2,3-epoxypropoxy)-N,N-bis(2,3-epoxypropyl)aniline test solution was 224.3 mg/l (t=0h).
The ratio of inoculum and m-(2,3-epoxypropoxy)-N,N-bis(2,3-epoxypropyl)aniline (as DOC) was 3:1.
2) Blank control mixture (1 vessel):
500 ml mineral medium
500 ml activated sludge (final concentration in test mixture: 0.75 g SS/l)
Made up to 2 litres with mineral medium.
3) Reference substance (1 vessel):
500 ml mineral medium
500 ml activated sludge (final concentration in test mixture: 0.75 g SS/l)
702.6 mg aniline.
Made up to 2 litres with mineral medium.
DOC of this solution (315.2 mg/l) was 253.3 mg/l (t=0h).
The ratio of inoculum and aniline (as DOC) was 3:1.
Prior to sampling the volume of the vessels was checked for evaporation and if necessary Milli-Q water was added. The medium was mixed thoroughly and a sample of 100 ml was taken. This sample was passed through a rough paper filter (S&S 604), and thereafter through a 0.45 µm filter (S&S FP 030/0.45). At least, the first 5 ml was discarded. 40 ml was used for DOC analysis. Another 40 ml was stored in a deep-freeze for possible analysis until delivery of the final report with a maximum of three months.
Frequency of sampling
- At the start of the test (0 h) and 3h after addition of the test substance in order to estimate any adsorption of m-(2,3-epoxypropoxy)-N,N-bis(2,3-epoxypropyl)aniline by the activated sludge;
- Day 1, 3, 7, 14, 21, 27 and 28.
Sample pretreatment
- Not applicable, samples were analysed on the day of sampling. The reserve samples were not analysed for this test.
% Degradation
- Parameter:
- % degradation (DOC removal)
- Value:
- 8
- Sampling time:
- 28 d
- Details on results:
- The results obtained during the test period showed no significant DOC removal in the first 3 hours of incubation in either of the test vessels indicating no or only limited adsorption to sludge during this period.
The biodegradation values calculated from the DOC measurements performed during the 28-day test period revealed no significant biodegradation of m-(2,3-epoxypropoxy)-N,N-bis(2,3-epoxypropyl)aniline. Thus, the biodegradation values did not reveal more than 20% biodegradation of m-(2,3-epoxypropoxy)-N,N-bis(2,3-epoxypropyl)aniline during the test period.
BOD5 / COD results
- Results with reference substance:
- The removal of aniline was at least 70% within 14 days (98%).
Any other information on results incl. tables
2nd Acceptability criterion:
The criterion for gradual removal of test substance during the test was not applicable as a maximum of 8% removal was observed in the test solution.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not inherently biodegradable
- Conclusions:
- m-(2,3-epoxypropoxy)-N,N-bis(2,3-epoxypropyl)aniline was not inherently biodegradable under the conditions of the 'Zahn-Wellens / EMPA Test' presently performed.
- Executive summary:
The inherent biodegradabilty of m-(2,3-epoxypropoxy)-N,N-bis(2,3-epoxypropyl)aniline under the conditions of the Zahn-Wellens/EMPA test was investigated according to OECD guideline 302 B and GLP principles. A single test concentration of 224,3 mg/L (as DOC) was tested during 28 days. Based on the obtained results, m-(2,3-epoxypropoxy)-N,N-bis(2,3-epoxypropyl)aniline was not inherently biodegradable. The study is considered to be reliable without restrictions.
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