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EC number: 947-754-5 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Key value for chemical safety assessment
Genetic toxicity in vitro
Description of key information
No effects were detectable in S. typhimurium tester strains following sublethal pH decrease (read-across).
Link to relevant study records
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- pH changes on bacterial reversion rate was evaluated by adopting 2 modifications of the standard plate incorporation assay
- GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
- Target gene:
- Histidine locus
- Species / strain / cell type:
- S. typhimurium TA 97
- Species / strain / cell type:
- S. typhimurium TA 98
- Species / strain / cell type:
- S. typhimurium TA 100
- Species / strain / cell type:
- S. typhimurium TA 102
- Species / strain / cell type:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor 1254 induced rat liver S9 was included but the study did not specify if it was used for this test
- Test concentrations with justification for top dose:
- up to toxic level, different pH’s (ranging from 4 to 9)
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- Positive controls:
- yes
- Positive control substance:
- methylmethanesulfonate
- Positive controls:
- yes
- Positive control substance:
- other: daunomycin
- Species / strain:
- S. typhimurium TA 97
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- The acidification of incubation mixture to pH 5.0 produced toxic effects on bacteria as the appearance of survivors suggested; at lower pH values, complete bacterial death was observed. Values between 5.5 and 9.0 do not exert significant effects.
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- The acidification of incubation mixture to pH 5.0 produced toxic effects on bacteria as the appearance of survivors suggested; at lower pH values, complete bacterial death was observed. Values between 5.5 and 9.0 do not exert significant effects.
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- The acidification of incubation mixture to pH 5.0 produced toxic effects on bacteria as the appearance of survivors suggested; at lower pH values, complete bacterial death was observed. Values between 5.5 and 9.0 do not exert significant effects.
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 102
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- The acidification of incubation mixture to pH 5.0 produced toxic effects on bacteria as the appearance of survivors suggested; at lower pH values, complete bacterial death was observed. Values between 5.5 and 9.0 do not exert significant effects.
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- The acidification of incubation mixture to pH 5.0 produced toxic effects on bacteria as the appearance of survivors suggested; at lower pH values, complete bacterial death was observed. Values between 5.5 and 9.0 do not exert significant effects.
- Positive controls validity:
- valid
- Additional information on results:
- The acidification of incubation mixture to pH 5.0 produced toxic effects on bacteria as the appearance of survivors suggested; at lower pH values, complete bacterial death was observed. Values between 5.5 and 9.0 do not exert significant effects.
The reversion properties and specificity of each strain were confirmed by testing MMS, daunomycin, and sodium azide in the standard plate-incorporation assay. - Conclusions:
- No effects were detectable in S. typhimurium tester strains following sublethal pH decrease.
- Executive summary:
The incubation of S. typhimurium tester strains with different buffer solutions at pH ranging from 5.5 to 9 had no effect on the bacterial reversion rates. The acidification of incubation mixture to pH 5.0 produced toxic effects on bacteria as the appearance of survivors suggested; at lower pH values, complete bacterial death was observed.
The same lack of effects was obtained by using the base agar plates at different pH values.
The ineffectiveness of pH decrease was invariably unchanged by the addition of S9 fraction, however no detailed results were reported.
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- This read-across is based on the hypothesis that source and target substances have similar properties because of structural similarity and that the target chemical (Amorphous condensation products of orthophosphoric acid and sodium carbonate (3-6 : 1)) hydrolyse to orthophosphoric acid and the sodium dihydrogen phosphate. The sodium dihydrogen phosphate has no identified hazardous properties. The orthophosphoric acid is source substance and is identical to the hydrolysis product of constituent A (metaphosphoric acid) of the target substance.
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- pH changes on bacterial reversion rate was evaluated by adopting 2 modifications of the standard plate incorporation assay
- GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
- Target gene:
- Histidine locus
- Species / strain / cell type:
- S. typhimurium TA 97
- Species / strain / cell type:
- S. typhimurium TA 98
- Species / strain / cell type:
- S. typhimurium TA 100
- Species / strain / cell type:
- S. typhimurium TA 102
- Species / strain / cell type:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor 1254 induced rat liver S9 was included but the study did not specify if it was used for this test
- Test concentrations with justification for top dose:
- up to toxic level, different pH’s (ranging from 4 to 9)
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- Positive controls:
- yes
- Positive control substance:
- methylmethanesulfonate
- Positive controls:
- yes
- Positive control substance:
- other: daunomycin
- Species / strain:
- S. typhimurium TA 97
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- The acidification of incubation mixture to pH 5.0 produced toxic effects on bacteria as the appearance of survivors suggested; at lower pH values, complete bacterial death was observed. Values between 5.5 and 9.0 do not exert significant effects.
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- The acidification of incubation mixture to pH 5.0 produced toxic effects on bacteria as the appearance of survivors suggested; at lower pH values, complete bacterial death was observed. Values between 5.5 and 9.0 do not exert significant effects.
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- The acidification of incubation mixture to pH 5.0 produced toxic effects on bacteria as the appearance of survivors suggested; at lower pH values, complete bacterial death was observed. Values between 5.5 and 9.0 do not exert significant effects.
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 102
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- The acidification of incubation mixture to pH 5.0 produced toxic effects on bacteria as the appearance of survivors suggested; at lower pH values, complete bacterial death was observed. Values between 5.5 and 9.0 do not exert significant effects.
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- The acidification of incubation mixture to pH 5.0 produced toxic effects on bacteria as the appearance of survivors suggested; at lower pH values, complete bacterial death was observed. Values between 5.5 and 9.0 do not exert significant effects.
- Positive controls validity:
- valid
- Additional information on results:
- The acidification of incubation mixture to pH 5.0 produced toxic effects on bacteria as the appearance of survivors suggested; at lower pH values, complete bacterial death was observed. Values between 5.5 and 9.0 do not exert significant effects.
The reversion properties and specificity of each strain were confirmed by testing MMS, daunomycin, and sodium azide in the standard plate-incorporation assay. - Conclusions:
- No effects were detectable in S. typhimurium tester strains following sublethal pH decrease (read-across).
- Executive summary:
The incubation of S. typhimurium tester strains with different buffer solutions at pH ranging from 5.5 to 9 had no effect on the bacterial reversion rates. The acidification of incubation mixture to pH 5.0 produced toxic effects on bacteria as the appearance of survivors suggested; at lower pH values, complete bacterial death was observed.
The same lack of effects was obtained by using the base agar plates at different pH values.
The ineffectiveness of pH decrease was invariably unchanged by the addition of S9 fraction, however no detailed results were reported.
Referenceopen allclose all
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (negative)
Additional information
Justification for classification or non-classification
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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