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EC number: 222-775-6 | CAS number: 3609-96-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- Materials and methods are well-documented in the publication. The study meets generally accepted scientific principles, but was not conducted in compliance with GLP and no analytical monitoring was carried out.
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- The test material was dissolved in water. The stock solution was added to the test chambers to archieve the desired nominal concentrations.
- Test organisms (species):
- Scenedesmus quadricauda
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 8 d
- Test temperature:
- 27 °C
- pH:
- 7 (pH of the water used for preparing the stock solution and diluting)
Adjustment of pH: not clear, it is stated that the solution of the test is neutralised unless the effect of the pH of the hazardous water pollutant solution to be studies is part of the test. - Details on test conditions:
- vessels per concentration (replicates): 3
vessels per control (replicates): 3 - Reference substance (positive control):
- not specified
- Key result
- Duration:
- 8 d
- Dose descriptor:
- other: Toxicity Threshold
- Effect conc.:
- 640 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Remarks:
- inhibition of cell division of the alga
- Key result
- Duration:
- 8 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 425 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Remarks:
- inhibition of cell division of the alga
- Remarks on result:
- other: derived from the toxicity threshold
- Key result
- Duration:
- 8 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 850 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Remarks:
- inhibition of cell division of the alga
- Remarks on result:
- other:
- Remarks:
- inhibition of cell division of the alga
- Details on results:
- Values were reported as toxicity threshold.
The inhibition of cell division caused by water ingredients is determined. The concentration of the algal suspension is measured turbidimetrically (while diffused light is screened off) and expressed by the extinction of the primary light of the monochromatic radiation at 578 nm for a layer of 10 mm thickness. The concentration at which the inhibitory action of a pollutant begins is present in that dilution from a series of dilutions of a pollutant having, at the end of the test period, a mean extinction value that is >= 3% below the mean value of the extinction value for non-toxic dilutions of the test cultures.
The mean value of the extinction is calculated for all test cultures that are free from both toxic influence and stimulation of growth except for those having extinction values outside a standard deviation of <3% and also, the mean value of the extinction for those test cultures having the lowest toxic pollutant concentration within the dilution series. - Validity criteria fulfilled:
- not specified
- Conclusions:
- For the algea Scenedesmus quadricauda an 8 day toxicity threshold value of 640 mg/L, a NOEC value of 425 mg/L and a LOEC value of 850 mg/L can be stated.
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Justification for type of information:
- Potassium citrate salts are known to show a very high solubility in water and dissociate rapidly.
Potassium in known to be non-toxic. The toxicity is determined by the citrate moiety of the substance.
Because of that a read-across to citric acid is suitable for the determination of the toxicity. - Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- Materials and methods are well-documented in the publication. The study meets generally accepted scientific principles, but was not conducted in compliance with GLP and no analytical monitoring was carried out.
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- The test material was dissolved in water. The stock solution was added to the test chambers to archieve the desired nominal concentrations.
- Test organisms (species):
- Scenedesmus quadricauda
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 8 d
- Test temperature:
- 27 °C
- pH:
- 7 (pH of the water used for preparing the stock solution and diluting)
Adjustment of pH: not clear, it is stated that the solution of the test is neutralised unless the effect of the pH of the hazardous water pollutant solution to be studies is part of the test. - Details on test conditions:
- vessels per concentration (replicates): 3
vessels per control (replicates): 3 - Reference substance (positive control):
- not specified
- Key result
- Duration:
- 8 d
- Dose descriptor:
- other: Toxicity Threshold
- Effect conc.:
- 640 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Remarks:
- inhibition of cell division of the alga
- Key result
- Duration:
- 8 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 425 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Remarks:
- inhibition of cell division of the alga
- Remarks on result:
- other: derived from the toxicity threshold
- Key result
- Duration:
- 8 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 850 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Remarks:
- inhibition of cell division of the alga
- Remarks on result:
- other:
- Remarks:
- inhibition of cell division of the alga
- Details on results:
- Values were reported as toxicity threshold.
The inhibition of cell division caused by water ingredients is determined. The concentration of the algal suspension is measured turbidimetrically (while diffused light is screened off) and expressed by the extinction of the primary light of the monochromatic radiation at 578 nm for a layer of 10 mm thickness. The concentration at which the inhibitory action of a pollutant begins is present in that dilution from a series of dilutions of a pollutant having, at the end of the test period, a mean extinction value that is >= 3% below the mean value of the extinction value for non-toxic dilutions of the test cultures.
The mean value of the extinction is calculated for all test cultures that are free from both toxic influence and stimulation of growth except for those having extinction values outside a standard deviation of <3% and also, the mean value of the extinction for those test cultures having the lowest toxic pollutant concentration within the dilution series. - Validity criteria fulfilled:
- not specified
- Conclusions:
- For the algea Scenedesmus quadricauda an 8 day toxicity threshold value of 640 mg/L, a NOEC value of 425 mg/L and a LOEC value of 850 mg/L can be stated.
Referenceopen allclose all
The study reports the result as a toxicity threshold (a value between the NOEC and the LOEC).
In order to derive the NOEC the toxicity threshold value is divided by 1.5, knowing that the spacing factor between exposure levels is 2.
In order to derive the LOEC the toxicity threshold value is multiplied by 1.5.
=> 640/1.5 = 425 mg/L (rounded to two significant figures) = NOEC
=> 640*1.5 = 850 mg/L (rounded to two significant figures) = LOEC
The study reports the result as a toxicity threshold (a value between the NOEC and the LOEC).
In order to derive the NOEC the toxicity threshold value is divided by 1.5, knowing that the spacing factor between exposure levels is 2.
In order to derive the LOEC the toxicity threshold value is multiplied by 1.5.
=> 640/1.5 = 425 mg/L (rounded to two significant figures) = NOEC
=> 640*1.5 = 850 mg/L (rounded to two significant figures) = LOEC
Description of key information
For the algea Scenedesmus quadricauda an 8 day toxicity threshold value of 640 mg/L, a NOEC value of 425 mg/L and a LOEC value of 850 mg/L can be stated.
Key value for chemical safety assessment
- EC10 or NOEC for freshwater algae:
- 425 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.