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Diss Factsheets
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EC number: 947-894-7 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- from 2018-05-08 to 2018-07-10
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 2004
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- (2-hydroxy-1,1-dimethylethyl)ammonium chloride
- EC Number:
- 221-713-5
- EC Name:
- (2-hydroxy-1,1-dimethylethyl)ammonium chloride
- Cas Number:
- 3207-12-3
- Molecular formula:
- C4H11NO.ClH
- IUPAC Name:
- 1-hydroxy-2-methylpropan-2-aminium chloride
- Test material form:
- liquid
1
Sampling and analysis
- Analytical monitoring:
- no
- Details on sampling:
- Due to complexity of test item, test concentration analysis was not possible.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Stock solution was freshly prepared prior to exposure by weighing required quantity of test item in a beaker. A small volume of reconstituted water was added and mixed well using a glass rod. After the complete solubility of the test item, the stock solution was transferred into a measuring cylinder. A magnetic stirrer was used to maintain the homogeneity of the stock solution. The required test concentrations were prepared by dilution of stock solution with reconstituted water.
- Controls: Positive control performed with potassium dichromate and blank control performed with the test solutions without test substance.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Waterflea
- Strain: Straus
- Source: Environmental and Molecular Toxicology Division, Department of Zoology, Karnataka Uni
versity, Karnataka, India
- Feeding during test: no
ACCLIMATION
- Acclimation period: 48 hours prior to test
- Acclimation conditions: same as test conditions
- Type and amount of food: live algal cells (Pseudokirchneriella subcapitata)
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Remarks on exposure duration:
- None
- Post exposure observation period:
- Not performed
Test conditions
- Hardness:
- 140 to 250 mg/L (as CaCO3)
- Test temperature:
- 18 to 22 °C
- pH:
- 6.0 to 9.0
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- - Nominal concentrations: of 0.6, 1.3, 2.8, 6.1, 13.4, 29.5 and 65 mg/L
- Measured concentrations: No concentrations were measured - Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass beakers
- Type: Open
- Volume: 50 mL
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dilution water prepared according with OECD guideline
- Culture medium different from test medium: No
- Intervals of water quality measurement: Total hardness of the reconstituted water was analyzed prior to its use for exposure. Temperature, pH and dissolved oxygen was recorded at the beginning and at the end of the test in controls and in all the test substance concentrations.
OTHER TEST CONDITIONS
- Adjustment of pH:No
- Photoperiod: 16 hours of light and 8 hours dark cycle
EFFECT PARAMETERS MEASURED: Immobilisation rate of daphnids by exposing them to the test item for 48 h.
VEHICLE CONTROL PERFORMED: No
RANGE-FINDING STUDY
- Test concentrations: - Nominal concentrations of 0.0131, 0.131, 1.31, 13.10, 65.5 and 131 mg/L
- Results used to determine the conditions for the definitive study: During dose range finding study, no immobility was observed in control and in the tested concentrations of 0.013 mg/L during the 48 hour observation period. % immobility of 10, 20, 50, 80 and 100% was observed in the tested concentrations of 0.131, 1.31, 13.1, 65.5 and 131.0 mg/L (test concentrations calculated using the correction factor of 1.31) during the 48 hour observation period.
Based on results of the dose range finding study, the main study will be conducted at the concentrations of 0.6, 1.3, 2.8, 6.1, 13.4, 29.5 and 65 mg/L. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrations
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 17.31 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% confidence intervals: 9.156 to 32.737 mg/L
- Details on results:
- Based on the results of the experiment, it can be concluded that the 48 hour EC50 of the test item on Daphnia magna is determined to be 17.313 mg/L.
- Results with reference substance (positive control):
- The 48-hour EC50 of the reference substance potassium dichromate on Daphnia magna was determined to be 0.57 mg/L. The 48 hour EC50 of the reference substance was within the acceptance range of the guideline (0.50 - 2.12 mg/L) confirming the suitability of the test system.
- Reported statistics and error estimates:
- EC10, EC20 and EC50 was calculated, using the percent immobility data of 0, 15, 25, 30, 50 and 100 % obtained for respective test concentrations of 0.6, 1.3, 2.8, 6.1, 13.4, 29.5 and 65 mg/L. The statistical analysis was carried out using ToxRatPro Version 3.2.1, software.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on the results of the experiment, it can be concluded that the 48 hour EC50 of the test item on Daphnia magna is determined to be 17.313 mg/L.
- Executive summary:
In a static immobilization test Daphnia magna (< 24 h old) were exposed to the test item for 48 hours according the OECD Guideline No. 202 (1992).
Daphnids were exposed to the test item by aqueous exposure (treatment) under static condition. Daphnids were observed at 24 and 48 hours for signs of toxicity and immobility. Based on the results of the range finding study, the main study was conducted at the nominal test concentrations of 0.6, 1.3, 2.8, 6.1, 13.4, 29.5 and 65 mg/L. Due to the complexity of the test item, test concentration analysis was not possible. The 48-hour EC50 of the reference substance potassium dichromate on Daphnia magna was determined to be 0.57 mg/L. Thus, the 48 hour EC50 of the reference substance was within the acceptance range of the guideline (0.50 - 2.12 mg/L) confirming the suitability of the test system. In addition, dissolved oxygen concentration at the end of the test was 6.27 mg/L and the immobilization in the control group was 0 % at the termination of the test during main study, therefore the validity criteria were met. Based on the results of the experiment, the 48h-EC50 was determined to be 17.313 mg/L.
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