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EC number: 807-159-2
CAS number: 69701-99-1
Results after 10 Minutes Incubation Time
Opacity value = Difference (t130-t0) of Opacity
Permeability at 490 nm (OD490)
Proposedin vitroIrritancy Score
* corrected values
This in vitro study was performed to assess the corneal damage potential
of MEDOL-10 by means of the BCOP assay using fresh bovine corneae. After
a first opacity measurement of the fresh bovine corneae (t0), the neat
test item, the positive, and the negative controls were applied to
corneae fixed in an incubation chamber in horizontal position for 10
minutes at 32 ± 1 °C. The posterior chamber contained incubation medium.
After the incubation phase the test item, the positive, and the negative
controls were each rinsed from the corneae. Further, the corneae were
incubated for another 120 minutes at 32 ± 1 °C in a vertical position,
while the anterior chamber contain incubation medium as well.
Afterwards, opacity was measured a second time (t130). After the opacity
measurements permeability of the corneae was determined by measuring
spectrophotometrically the transfer of sodium fluorescein after
incubation in a horizontal position for 90 minutes at 32 ± 1 °C. With
the negative control (0.9% (w/v) NaCl solution in deionised water)
neither an increase of opacity nor permeability of the corneae could be
observed. The positive control (2-Ethoxyethanol) showed clear opacity
and distinctive permeability of the corneae corresponding to a
classification as serious eye damaging (CLP/EPA/GHS (Cat 1)).
Relative to the negative control, the test item MEDOL-10 did not cause
an increase of the corneal opacity or permeability. The calculated mean
in vitro irritancy score was 2.03. According to OECD 437 the test item
is not categorized (GHS).
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