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EC number: 603-020-3 | CAS number: 12501-23-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2017-09-19 to 2017-12-07
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Principles of method if other than guideline:
- There were no deviations and no amendments to this study.
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Batch/Lot no: 160306
Purity: >99% - Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations:
- Sampling method: approximately 40 mL of each test solution and the negative control were collected in glass containers of appropriate size
- Sample storage conditions before analysis: stored refrigerated (2°C to 8°C) until analysis - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: a stock solution was prepared at 100 mg test item/L and test solutions were prepared as dilutions of this stock
- Controls: negative control (in parallel), positive control (in a separate non-GLP study)
- Evidence of undissolved material: no - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Source: Aquatic BioSystems (Fort Collins, CO, USA), in-house culture
- Age of parental stock: less than 24 hours old
- Feeding during test: no; only during culturing
- Food type: Pseudokirchneriella subcapitata and 1.0 mL of yeast-cereal-grass fermented trout chow
- Amount: 4.0 mL of Pseudokirchneriella subcapitata and 1.0 mL of yeast-cereal-grass fermented trout chow
- Frequency: three times per week
METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES:
Daphnids were randomly selected and transferred to each of the four replicates of the negative control, using a wide-bore glass pipette - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Remarks on exposure duration:
- according to guideline
- Post exposure observation period:
- no
- Hardness:
- 92 mg/L CaCO3
- Test temperature:
- 19.9 – 20.5 °C
- pH:
- 7.3 – 8.1
- Dissolved oxygen:
- 8.9 – 9.0 mg/L
- Salinity:
- not applicable
- Conductivity:
- 325 (negative control), 321 (6.25 mg/L), 320 (12.5 mg/L), 319 (25 mg/L), 318 (50 mg/L) and 314 (100 mg/L) µS/cm
- Nominal and measured concentrations:
- Nominal: 6.25, 12.5, 25, 50, and 100 mg/L
Measured mean concentrations of Tungsten in test solution: 4.1, 8.4, 16.6, 29.7 and 51.6 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: beakers
- Type: closed with Plexiglas
- Material, size, headspace, fill volume: glass, 250 mL, 200 mL test volume
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: moderately hard, reconstituted water for culturing
- Particulate matter: no
- Water quality measurements: prior to use
- Culture medium different from test medium: no
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16-h light : 8-h dark with a 30 minute transition period
- Light intensity: 609 – 679 lux
EFFECT PARAMETERS MEASURED
- Immobility
RANGE-FINDING STUDY
- Test concentrations: 0, 0.1, 1.0, 10, and 100 mg test item/L
- Results used to determine the conditions for the definitive study: yes - Reference substance (positive control):
- yes
- Remarks:
- zinc sulphate heptahydrate (CAS no. 7446-20-0)
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 70.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% confidence limits: 56.6 - 84.6 mg/L
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 50 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities: no
- Observations on body length and weight: no
- Other biological observations: no
- Mortality of control: no
- Other adverse effects control: no
- Abnormal responses: no
- Any observations that might cause a difference between measured and nominal values: no
- Effect concentrations exceeding solubility of substance in test medium: no - Results with reference substance (positive control):
- The 48-hours LC50 and 95% confidence limits for the reference item test are 0.77 mg/L (0.48, 1.60 mg/L). This value indicates that the test organisms responded normally to the reference item and confirms the suitability of the test system.
- Reported statistics and error estimates:
- The number of immobilised daphnids at the 24 ± 1hours and 48 ±1 hours time points was calculated using Microsoft Excel and graphically plotted against the negative control and nominal test item concentrations to create a concentration response curve. The mean and standard deviation for the negative control and each nominal test item concentration was calculated, where appropriate, as per the OECD 202 Test Guideline.
The 48-hour EC50 with 95% confidence limits (based on nominal concentrations) and the NOEC and LOEC values for immobilisation were calculated using a computerised statistical package, CETIS™ (Version 1.9.2.4), a Comprehensive Environmental Toxicity Information System, recommended by Environment Canada. All reported values were rounded according to the Work Instruction for Significant Figures in Data Reporting (COR WI-00114). - Validity criteria fulfilled:
- yes
Reference
Table 1: Number and percentage of Daphnia immobilised after 24-h exposure
Nominal Concentration of Test Item |
Mean |
Replicate |
Replicate |
Replicate |
Replicate |
Negative Control (0) |
0 ± 0 |
0 / 0 |
0 / 0 |
0 / 0 |
0 / 0 |
6.25 |
0 ± 0 |
0 / 0 |
0 / 0 |
0 / 0 |
0 / 0 |
12.5 |
0 ± 0 |
0 / 0 |
0 / 0 |
0 / 0 |
0 / 0 |
25 |
0 ± 0 |
0 / 0 |
0 / 0 |
0 / 0 |
0 / 0 |
50 |
0 ± 0 |
0 / 0 |
0 / 0 |
0 / 0 |
0 / 0 |
100 |
0 ± 0 |
0 / 0 |
0 / 0 |
0 / 0 |
0 / 0 |
Table 2: Number and percentage ofDaphnia immobilised after 48-h exposure
Nominal Concentration of Test Item |
Mean |
Replicate |
Replicate |
Replicate |
Replicate |
Negative Control (0) |
0 ± 0 |
0 / 0 |
0 / 0 |
0 / 0 |
0 / 0 |
6.25 |
0 ± 0 |
0 / 0 |
0 / 0 |
0 / 0 |
0 / 0 |
12.5 |
5 ± 10 |
0 / 0 |
0 / 0 |
0 / 0 |
1 / 20 |
25 |
5 ± 10 |
0 / 0 |
0 / 0 |
1 / 20 |
0 / 0 |
50 |
20 ± 23 |
2 / 40 |
0 / 0 |
2 / 40 |
0 / 0 |
100 |
80 ± 16 |
5 / 100 |
4 / 80 |
3 / 60 |
4 / 80 |
Table 3: Measured concentrations of Tungsten in test solutions
Nominal concentration of test item [mg/L] | Tungsten [mg/L] | |||
Initial | Final | Mean | SD | |
Negative control (0) | BQL | BQL | N/A | N/A |
6.25 | 4.1 | 4.1 | 4.1 | 0.0 |
12.5 | 8.2 | 8.6 | 8.4 | 0.3 |
25 | 15.4 | 17.8 | 16.6 | 1.7 |
50 | 25.5 | 33.9 | 29.7 | 5.9 |
100 | 43.2 | 60.1 | 51.6 | 12.0 |
BQL: Below Quantitation (<0.002 mg/L)
N/A: Not Applicable
Description of key information
The 48-hour EC50 of the test item was determined to be 70.8 mg/L (based on nominal concentrations).
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 70.8 mg/L
Additional information
The toxicity to aquatic invertebrates was assessed in Acute Immobilisation Test with Daphnia magna according to OECD Guideline 202 and in compliance with GLP principles. This static aquatic test was conducted under controlled conditions using less than 24 hours old Daphnia magna neonates over a 48-hour exposure period and was carried out using the following nominal concentrations: 6.25, 12.5, 25, 50, and 100 mg test Item/L, along with a negative control (0 mg/L). At test completion, there were no immobilised or dead daphnids in the negative control or in the 6.25 mg/L test item solutions. The remaining test item solutions had 5% - 80% immobilised or dead daphnids. For confirmation of test item concentrations, Tungsten was analysed as a proxy for the test item. Concentration of Tungsten in test solutions was consistent between test initiation and test completion, with values within ± 20% of the mean Tungsten concentration for each nominal test item concentration. Therefore, the biological test endpoint was calculated using the nominal concentrations of the test item. Based on the nominal concentrations of the test item, the 48-hour EC50 was 70.8 mg/L (95% confidence limits: 56.6 - 84.6 mg/L). The 48-hour NOEC and LOEC was determined to be 50 mg/L and 100 mg/L, respectively. All validity criteria of the guideline were fulfilled.
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