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EC number: 257-885-3 | CAS number: 52372-39-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
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- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
Skin irritation
Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the skin irritation potential of the test chemical 3-(diethylamino)-7-imino-7H-chromeno[3’,2’:3,4]pyrido[1,2-a]benzimidazole-6-carbonitrile (CAS No: 52372-39-1). The studies are as mentioned below:
The peer reviewed journal conductedskin irritation study of read across substance in guinea pigs. The10 female Pirbright-White guinea pigs were treated with an emulsion prepared from FCA and physiological saline but without the test chemical and applied on a semicircular arc on the clipped or shaved shoulder area (4*6 cm) from left to right in such a way that whole emulsion was used up for the animals.1 day before the challenge exposure, ten guinea pigs were treated with test chemical by applying different concentrations of the test chemical (10%, 3% and 1%) to the right flank. The results of the challenge exposure were observed after 24 hours. A (+) reaction was considered as threshold of irritation. The mean irritation index of chemical was 0.0 after 24 hours. Hence, the test material was considered to be not irritating to guinea pigs.
Above result was further supported by dermal irritation carried out byanother peer reviewed journal for another read across substance.The test uses a single rabbit ear to indicate the Comedogenicity and irritancy of test chemical. The chemical was mixed in propylene glycol at a 9 to 1 dilution for testing unless otherwise indicated (10% concentration). A colony of New Zealand albino rabbits that have genetically good ears and free from mites were used. Three rabbits, weighing two to three kilograms, were used for each assay. Animals were housed singly in suspended cages and fed Purina Rabbit Chow and water ad libitum. Animals were maintained on a 12-hour light and 12-hour dark cycle. A dose of 1 ml of the test material was applied and spread once daily to the entire inner surface of once for five days per week for two weeks. The opposite untreated ear of each animal served as an untreated control. The irritancy produced by repeated application of the chemical on the surface epidermis in the rabbit ear is evaluated on a scale of 0 to 5. The grades are summarized as follows: 0 = No irritation; 1 = few scales, no Erythema; 2 = diffuse scaling, no Erythema; 3 = Generalized scaling with Erythema; 4 = Scaling, Erythema and Edema; 5 = Epidermal necrosis and slough. The chemical falls under Grade 0 (no irritation observed). Hence it can be concluded that the test substance was not irritating to rabbit ears.
Based on the above summarized studies for target chemical 3-(diethylamino)-7-imino-7H-chromeno[3’,2’:3,4]pyrido[1,2-a]benzimidazole-6-carbonitrile (CAS No: 52372-39-1) and its structurally similar read across substances,it can be concluded that the testchemical is not able to cause skin irritation and considered as not irritating. Comparing the above annotations with the criteria of CLP regulation, it can be classified under the category “Not Classified.
Eye irritation
Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the ocular irritation potential of the test chemical 3-(diethylamino)-7-imino-7H-chromeno[3’,2’:3,4]pyrido[1,2-a]benzimidazole-6-carbonitrile (CAS No: 52372-39-1). The studies are as mentioned below:
The ocular irritation potential of the test substance was conducted by peer reviewed journal on rabbits. When the chemical was instilled into the conjunctival sac of each rabbit, none of the treated rabbit had developed any signs of ocular lesions. Hence the test chemical was considered to be not irritating to the rabbit’ eye.
The above result was further supported by another ocular irritation study reported by authoritative database for another read across chemical. In this test, each rabbit received undiluted chemical and were observed for any ocular lesions. Since the chemical did not induce any adverse effects, the test material was considered as not irritating to the rabbits’ eye.
Based on the above summarized studies for target chemical 3-(diethylamino)-7-imino-7H-chromeno[3’,2’:3,4]pyrido[1,2-a]benzimidazole-6-carbonitrile (CAS No: 52372-39-1) and its structurally similar read across substances,it can be concluded that the testchemical is not able to cause eye irritation and considered as not irritating. Comparing the above annotations with the criteria of CLP regulation, it can be classified under the category “Not Classified”.
Key value for chemical safety assessment
Skin irritation / corrosion
Link to relevant study records
- Endpoint:
- skin irritation: in vivo
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Remarks:
- experimental data of read across substances
- Justification for type of information:
- Data for the target chemical is summarized based on the structurally similar read across chemicals
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- other: as mentioned below
- Principles of method if other than guideline:
- WoE report is based on 2 skin irritation studies as- WoE-2 and WoE-3.
Skin irritation study of test chemical was conducted on guinea pigs and rabbits to assess its skin irritating effects. - GLP compliance:
- not specified
- Specific details on test material used for the study:
- - Name of test material : 3-(diethylamino)-7-imino-7H-[1]benzopyrano[3',2':3,4]pyrido[1,2-a]benzimidazole-6-carbonitrile
- Molecular formula : C23H19N5O
- Molecular weight : 381.437 g/mol
- Smiles notation : c12cc3c4nc5ccccc5n4c(=N)c(C#N)c3oc1cc(N(CC)CC)cc2
- InChl : 1S/C23H19N5O/c1-3-27(4-2)15-10-9-14-11-16-21(29-20(14)12-15)17(13-24)22(25)28-19-8-6-5-7-18(19)26-23(16)28/h5-12,25H,3-4H2,1-2H3
- Substance type : Organic
- Physical state : Solid - Species:
- other: 1. guinea pig 2.Rabbit
- Strain:
- other: 1.Pirbright- White 2.New Zealand White
- Details on test animals or test system and environmental conditions:
- 1.TEST ANIMALS
- Sex: female
- Age at study initiation: No Data available
- Weight at study initiation:280-350 g
- Housing: animals were kept 3 to a cage
- Diet (e.g. ad libitum):altromin ad libitum
- Water (e.g. ad libitum): water ad libitum
- Acclimation period: No Data available
ENVIRONMENTAL CONDITIONS
- Temperature (°C):22-24⁰C
- Humidity (%):50-55%
- Air changes (per hr): No Data available
- Photoperiod (hrs dark / hrs light): Under artificial illumination for 10 hours a day
2.TEST ANIMALS
Source: No data
Age at study initiation: No data
Weight at study initiation: 3 kg
Fasting period before study: No data
Housing: singly housed, in suspended cages
Diet (e.g. ad libitum): Purina Rabbit Chow, ad libitum
Water (e.g. ad libitum): water, ad libitum
Acclimation period: No data
ENVIRONMENTAL CONDITIONS
Temperature (°C): No data
Humidity (%):No data
Air changes (per hr): No data
Photoperiod (hrs dark / hrs light): 12 hour light and 12 hr dark cycle
IN-LIFE DATES: From: No data To: No data - Type of coverage:
- other: 1. open 2.Entire inner surface of one ear
- Preparation of test site:
- other: 1.clipped or shaved 2.A colony of rabbits that had genetically good ears and which were free from mites were used for the assay
- Vehicle:
- other: 1.FCA and Physiological saline 2.propylene glycol
- Controls:
- not specified
- Amount / concentration applied:
- 1.10%, 3% and 1 %
2.Dose of 1ml of the test material was applied once daily for five days per week for 2 weeks. - Duration of treatment / exposure:
- 1.24 hours
2.2 weeks - Observation period:
- 1.24 hours
2.2 weeks - Number of animals:
- 1.10
2.3 rabbits - Details on study design:
- 1.TEST SITE
- Area of exposure: clipped or shaved shoulder area (4*6 cm)
OBSERVATION TIME POINTS
(indicate if minutes, hours or days) : The results were read 24 hours after challenge exposure with the test chemical
SCORING SYSTEM:
- Method of calculation:A (+) reaction was considered as threshold of irritation
2.TEST SITE
Area of exposure: Ear
% coverage: A dose of 1ml of the test chemical is applied and spread once daily to the entire inner surface of one ear five days per week for 2 weeks.
Type of wrap if used: No data
REMOVAL OF TEST SUBSTANCE
Washing (if done): No data
Time after start of exposure: No data
SCORING SYSTEM: The irritancy produced by repeated application of a chemical or skin care product on the surface epidermis in the rabbit ear is evaluated on a scale of 0 to 5. The grades are summarized as follows:
0 = No irritation
1 = few scales, no Erythema
2 = diffuse scaling, no Erythema
3 = Generalized scaling with Erythema
4 = Scaling, Erythema and Edema
5 = Epidermal necrosis and slough - Irritation parameter:
- overall irritation score
- Basis:
- mean
- Time point:
- 24 h
- Score:
- 0
- Reversibility:
- not specified
- Remarks on result:
- no indication of irritation
- Irritation parameter:
- overall irritation score
- Basis:
- mean
- Time point:
- 14 d
- Score:
- 0
- Max. score:
- 5
- Reversibility:
- not specified
- Remarks on result:
- no indication of irritation
- Irritant / corrosive response data:
- 1.No skin irritation observed
2.No erythema observed - Interpretation of results:
- other: not irritating
- Conclusions:
- The test chemical 3-(diethylamino)-7-imino-7H-chromeno[3’,2’:3,4]pyrido[1,2-a]benzimidazole-6-carbonitrile (CAS No: 52372-39-1) was considered as not irritating to the skin.
- Executive summary:
Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the skin irritation potential of the test chemical 3-(diethylamino)-7-imino-7H-chromeno[3’,2’:3,4]pyrido[1,2-a]benzimidazole-6-carbonitrile (CAS No: 52372-39-1). The studies are as mentioned below:
The peer reviewed journal conductedskin irritation study of read across substance in guinea pigs. The10 female Pirbright-White guinea pigs were treated with an emulsion prepared from FCA and physiological saline but without the test chemical and applied on a semicircular arc on the clipped or shaved shoulder area (4*6 cm) from left to right in such a way that whole emulsion was used up for the animals.1 day before the challenge exposure, ten guinea pigs were treated with test chemical by applying different concentrations of the test chemical (10%, 3% and 1%) to the right flank. The results of the challenge exposure were observed after 24 hours. A (+) reaction was considered as threshold of irritation. The mean irritation index of chemical was 0.0 after 24 hours. Hence, the test material was considered to be not irritating to guinea pigs.
Above results were further supported by dermal irritation carried out byanother peer reviewed journal for another read across substance.The test uses a single rabbit ear to indicate the Comedogenicity and irritancy of test chemical. The chemical was mixed in propylene glycol at a 9 to 1 dilution for testing unless otherwise indicated (10% concentration). A colony of New Zealand albino rabbits that have genetically good ears and free from mites were used. Three rabbits, weighing two to three kilograms, were used for each assay. Animals were housed singly in suspended cages and fed Purina Rabbit Chow and water ad libitum. Animals were maintained on a 12-hour light and 12-hour dark cycle. A dose of 1 ml of the test material was applied and spread once daily to the entire inner surface of once for five days per week for two weeks. The opposite untreated ear of each animal served as an untreated control. The irritancy produced by repeated application of the chemical on the surface epidermis in the rabbit ear is evaluated on a scale of 0 to 5. The grades are summarized as follows: 0 = No irritation; 1 = few scales, no Erythema; 2 = diffuse scaling, no Erythema; 3 = Generalized scaling with Erythema; 4 = Scaling, Erythema and Edema; 5 = Epidermal necrosis and slough. The chemical falls under Grade 0 (no irritation observed). Hence it can be concluded that the test substance was not irritating to rabbit ears.
Based on the above summarized studies for target chemical 3-(diethylamino)-7-imino-7H-chromeno[3’,2’:3,4]pyrido[1,2-a]benzimidazole-6-carbonitrile (CAS No: 52372-39-1) and its structurally similar read across substances,it can be concluded that the testchemical is not able to cause skin irritation and considered as not irritating. Comparing the above annotations with the criteria of CLP regulation, it can be classified under the category “Not Classified.
Reference
1.Table 1: Results of the study
Test chemical |
Concentration |
Irritation index |
Test chemical |
10% |
0.0 |
The mean irritation index is the average of 3 readings
2.
Table 1: Ingredients and their Comedogenicity and Irritancy
Test chemical |
Comedogenicity* [Grade 0-5] |
Irritancy** [Grade 0-5] |
Test chemical |
2 |
0 |
*Comedogenicity or the ability of test substance to produce follicular hyperkeratosis
** Irritancy or ability of test substance to produce surface epithelial irritation
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (not irritating)
Eye irritation
Link to relevant study records
- Endpoint:
- eye irritation: in vivo
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Remarks:
- experimental data of read across substances
- Justification for type of information:
- Data for the target chemical is summarized based on the structurally similar read across chemicals
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- other: as mentioned below
- Principles of method if other than guideline:
- WoE report is based on 2 eye irritation studies as- WoE-2 and WoE-3.
An eye irritation study of test chemical was conducted on rabbits to assess its eye irritating effects. - Specific details on test material used for the study:
- - Name of test material : 3-(diethylamino)-7-imino-7H-[1]benzopyrano[3',2':3,4]pyrido[1,2-a]benzimidazole-6-carbonitrile
- Molecular formula : C23H19N5O
- Molecular weight : 381.437 g/mol
- Smiles notation : c12cc3c4nc5ccccc5n4c(=N)c(C#N)c3oc1cc(N(CC)CC)cc2
- InChl : 1S/C23H19N5O/c1-3-27(4-2)15-10-9-14-11-16-21(29-20(14)12-15)17(13-24)22(25)28-19-8-6-5-7-18(19)26-23(16)28/h5-12,25H,3-4H2,1-2H3
- Substance type : Organic
- Physical state : Solid - Species:
- rabbit
- Strain:
- not specified
- Details on test animals or tissues and environmental conditions:
- not specified
- Vehicle:
- other: 1. not specified 2.unchanged
- Controls:
- not specified
- Amount / concentration applied:
- 1.not specified
2. 100% (undiluted) - Duration of treatment / exposure:
- not specified
- Observation period (in vivo):
- not specified
- Duration of post- treatment incubation (in vitro):
- not specified
- Number of animals or in vitro replicates:
- not specified
- Details on study design:
- not specified
- Irritation parameter:
- overall irritation score
- Basis:
- mean
- Reversibility:
- not specified
- Remarks on result:
- no indication of irritation
- Irritant / corrosive response data:
- No eye irritation was observed in treated group.
- Interpretation of results:
- other: not irritating
- Conclusions:
- The test material 3-(diethylamino)-7-imino-7H-chromeno[3’,2’:3,4]pyrido[1,2-a]benzimidazole-6-carbonitrile (CAS No: 52372-39-1) was considered to be not irritating to the rabbits' eye.
- Executive summary:
Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the ocular irritation potential of the test chemical 3-(diethylamino)-7-imino-7H-chromeno[3’,2’:3,4]pyrido[1,2-a]benzimidazole-6-carbonitrile (CAS No: 52372-39-1). The studies are as mentioned below:
The ocular irritation potential of the test substance was conducted by peer reviewed journal on rabbits. When the chemical was instilled into the conjunctival sac of each rabbit, none of the treated rabbit had developed any signs of ocular lesions. Hence the test chemical was considered to be not irritating to the rabbit’ eye.
The above result was further supported by another ocular irritation study reported by authoritative database for another read across chemical. In this test, each rabbit received undiluted chemical and were observed for any ocular lesions. Since the chemical did not induce any adverse effects, the test material was considered as not irritating to the rabbits’ eye.
Based on the above summarized studies for target chemical 3-(diethylamino)-7-imino-7H-chromeno[3’,2’:3,4]pyrido[1,2-a]benzimidazole-6-carbonitrile (CAS No: 52372-39-1) and its structurally similar read across substances,it can be concluded that the testchemical is not able to cause eye irritation and considered as not irritating. Comparing the above annotations with the criteria of CLP regulation, it can be classified under the category “Not Classified”.
Reference
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (not irritating)
Respiratory irritation
Endpoint conclusion
- Endpoint conclusion:
- no study available
Additional information
Skin irritation
Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the skin irritation potential of the test chemical 3-(diethylamino)-7-imino-7H-chromeno[3’,2’:3,4]pyrido[1,2-a]benzimidazole-6-carbonitrile (CAS No: 52372-39-1). The studies are as mentioned below:
The peer reviewed journal conductedskin irritation study of read across substance in guinea pigs. The10 female Pirbright-White guinea pigs were treated with an emulsion prepared from FCA and physiological saline but without the test chemical and applied on a semicircular arc on the clipped or shaved shoulder area (4*6 cm) from left to right in such a way that whole emulsion was used up for the animals.1 day before the challenge exposure, ten guinea pigs were treated with test chemical by applying different concentrations of the test chemical (10%, 3% and 1%) to the right flank. The results of the challenge exposure were observed after 24 hours. A (+) reaction was considered as threshold of irritation. The mean irritation index of chemical was 0.0 after 24 hours. Hence, the test material was considered to be not irritating to guinea pigs.
Above result was further supported by dermal irritation carried out byanother peer reviewed journal for another read across substance.The test uses a single rabbit ear to indicate the Comedogenicity and irritancy of test chemical. The chemical was mixed in propylene glycol at a 9 to 1 dilution for testing unless otherwise indicated (10% concentration). A colony of New Zealand albino rabbits that have genetically good ears and free from mites were used. Three rabbits, weighing two to three kilograms, were used for each assay. Animals were housed singly in suspended cages and fed Purina Rabbit Chow and water ad libitum. Animals were maintained on a 12-hour light and 12-hour dark cycle. A dose of 1 ml of the test material was applied and spread once daily to the entire inner surface of once for five days per week for two weeks. The opposite untreated ear of each animal served as an untreated control. The irritancy produced by repeated application of the chemical on the surface epidermis in the rabbit ear is evaluated on a scale of 0 to 5. The grades are summarized as follows: 0 = No irritation; 1 = few scales, no Erythema; 2 = diffuse scaling, no Erythema; 3 = Generalized scaling with Erythema; 4 = Scaling, Erythema and Edema; 5 = Epidermal necrosis and slough. The chemical falls under Grade 0 (no irritation observed). Hence it can be concluded that the test substance was not irritating to rabbit ears.
Based on the above summarized studies for target chemical 3-(diethylamino)-7-imino-7H-chromeno[3’,2’:3,4]pyrido[1,2-a]benzimidazole-6-carbonitrile (CAS No: 52372-39-1) and its structurally similar read across substances,it can be concluded that the testchemical is not able to cause skin irritation and considered as not irritating. Comparing the above annotations with the criteria of CLP regulation, it can be classified under the category “Not Classified.
Eye irritation
Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the ocular irritation potential of the test chemical 3-(diethylamino)-7-imino-7H-chromeno[3’,2’:3,4]pyrido[1,2-a]benzimidazole-6-carbonitrile (CAS No: 52372-39-1). The studies are as mentioned below:
The ocular irritation potential of the test substance was conducted by peer reviewed journal on rabbits. When the chemical was instilled into the conjunctival sac of each rabbit, none of the treated rabbit had developed any signs of ocular lesions. Hence the test chemical was considered to be not irritating to the rabbit’ eye.
The above result was further supported by another ocular irritation study reported by authoritative database for another read across chemical. In this test, each rabbit received undiluted chemical and were observed for any ocular lesions. Since the chemical did not induce any adverse effects, the test material was considered as not irritating to the rabbits’ eye.
Based on the above summarized studies for target chemical 3-(diethylamino)-7-imino-7H-chromeno[3’,2’:3,4]pyrido[1,2-a]benzimidazole-6-carbonitrile (CAS No: 52372-39-1) and its structurally similar read across substances,it can be concluded that the testchemical is not able to cause eye irritation and considered as not irritating. Comparing the above annotations with the criteria of CLP regulation, it can be classified under the category “Not Classified”.
Justification for classification or non-classification
The skin and eye irritation potential of test chemical 3-(diethylamino)-7-imino-7H-chromeno[3’,2’:3,4]pyrido[1,2-a]benzimidazole-6-carbonitrile (CAS No: 52372-39-1) and its structurally similar read across substanceswere observed in various studies. The results obtained from these studies indicate that the chemical is unlikely to cause skin and eye irritation. Hence 3-(diethylamino)-7-imino-7H-chromeno[3’,2’:3,4]pyrido[1,2-a]benzimidazole-6-carbonitrile (CAS No: 52372-39-1) can be classified under the category “Not Classified” for skin and eye as per CLP.
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