Glycine, N-coco acyl derivs., sodium salts

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

17.12.1998 to 03.02.1999

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1999

Report date:

1999

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

other: Kanpogyou No. 5, Yakuhatsu No. 615 and 49 Kikyoku No. 392, 1974

Test guideline 2

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))

GLP compliance:

yes

Remarks:

Yokohama Laboratory, Mitsubishi Chemical Safety Institue Ltd. (M.S.I.), 1000 Kamshida-cho, Aoba-ku, Yokohama, Japan

Test material

Test material information

Constituent 1

Reference substance name:

Glycine, N-coco acyl derivs., sodium salts

EC Number:

291-350-5

EC Name:

Glycine, N-coco acyl derivs., sodium salts

Cas Number:

90387-74-9

Molecular formula:

Molecular formula for this UVCB is not available.

IUPAC Name:

Glycine, N-coco acyl derivs., sodium salts

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Ajinomoto Co., Inc., Tokyo (Japan)//lot No.: 980119
- Expiration date of the lot/batch: not reported (Date of receipt: 10.11.1998)
- Purity test date: not reported

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material:not reported (storage stability of test item over the exposure period of the test was proven by IR spectrum)

TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing:no
- Preliminary purification step (if any): no

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Activated sludge was obtained from the Chemical Biotesting Center, Chemical Inspection and Testing Institute, Japan
- Laboratory culture: yes
- Method of cultivation: not reported
- Storage conditions: not reported
- Storage length: not reported
- Preparation of inoculum for exposure: not reported
- Pretreatment: no
- Concentration of sludge: 30 mg/L

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

100 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
- Composition of medium: not specified
- Additional substrate: no
- Test temperature: 25 +/- 1°C
- pH: at day 28 in test vessels with test item: 7.1-7.3 (pH 6.2 in abiotic control of test item; aniline: pH 7.9)
- pH adjusted: not reported
- Aeration of dilution water: not reported
- Continuous darkness: not reported
- Other: amount of activated sludge in test vessels: 30 mg/L

TEST SYSTEM
- Culturing apparatus: Closed system oxygen consumption measuring apparatus OM-2001 (respirometer)
- Number of culture flasks/concentration: 6
- Method used to create aerobic conditions: not reported
- Measuring equipment: pH meter; TOC analyzer TOC-5000 (Shimadzu Co.); HPLC for measuring the test item concentration in the test bottles at the end of the exposure period
- Test performed in closed vessels: yes

SAMPLING
- Sampling frequency: at day 7, 14, 21, and 28
- Other: after termination of the test, DOC and the residual concentration of test substance in the test solution were determined

CONTROL AND BLANK SYSTEM
- Inoculum blank: performed
- Abiotic sterile control: performed
- Toxicity control: not performed
- Other: activity control performed with reference substance aniline

Reference substance

Reference substance:

aniline

Remarks:

100 mg/L

Results and discussion

% Degradation

% Degradation 1

Key result

Parameter:

% degradation (O2 consumption)

Value:

ca. 72

Sampling time:

7 d

% Degradation 2

Key result

Parameter:

% degradation (O2 consumption)

Value:

ca. 80

Sampling time:

28 d

% Degradation 3

Key result

Parameter:

% degradation (DOC removal)

Value:

99

Sampling time:

28 d

% Degradation 4

Key result

Parameter:

% degradation (test mat. analysis)

Value:

> 98

Sampling time:

28 d

BOD5 / COD results

Results with reference substance:

Based on BOD measurements, the reference substance aniline was biodegraded by 67% after 7 d.

Applicant's summary and conclusion

Interpretation of results:

readily biodegradable

Conclusions:

The test item proved to be readily biodegradable: The mean biodegradation of the test item after 28 d was determined to be 80% based on BOD, 99% based on DOC, and >98% based on residual concentration in the test vessels.

Executive summary:

The ready biodegradation of the the test item was investigated in a study conducted in accordance with the Test Method Relating to New Chemical Substances (Kanpogyou No. 5, Yakuhatsu No. 615 and 49 Kikyoku No. 392, 1974) as required by the Chemical Substances Control Law of Japan. The test procedure followed is equivalent or similar to OECD Guideline 301C (Ready Biodegradability: Modified MITI Test (I)). The test item (100 mg/L) was inoculated with activated sludge (30 mg/L) for 28 d and the degradation of the test item was determined by measurement of oxygen consumption BOD, DOC and residual test item concentration in the test vessels at the end of the test. Inoculum blank, abiotic control and procedural/functional control with the reference substance aniline (100 mg/L) were performed.

 

This study is regarded as reliable with restrictions due to missing informations in respect to e.g. origin of activated sludge, composition of basal medium etc..

 

The test item proved to be readily biodegradable ( 80% based on BOD, 99% based on DOC, and >98% based on residual concentration in the test vessels after 28 d of incubation at test substance concentrations of 100 mg/L). The functional control aniline reached the pass level > 60% after 7 d.