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EC number: 247-361-2 | CAS number: 25952-53-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Hydrolysis
Administrative data
Link to relevant study record(s)
- Endpoint:
- hydrolysis
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 13 September 2017 to 07 December 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 111 (Hydrolysis as a Function of pH)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.7 (Degradation: Abiotic Degradation: Hydrolysis as a Function of pH)
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 835.2120 (Hydrolysis of Parent and Degradates as a Function of pH at 25°C)
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- - Source and lot/batch No.of test material: Sponsor and AAN0680
- Expiration date of the lot/batch: 30 August 2018
- Purity test date: 09 September 2016 - Radiolabelling:
- no
- Analytical monitoring:
- no
- Buffers:
- Acetate buffer pH 4, 0.01 M Solution of 16.7% 0.01 M sodium acetate in water and 83.3% 0.01 M acetic acid in water. Buffer contained 0.0009% (w/v) sodium azide.
Phosphate buffer pH 7, 0.01 M Solution of 0.01 M potassium di-hydrogen-phosphate in water adjusted to pH 7 using 1N sodium hydroxide. Buffer contained 0.0009% (w/v) sodium azide.
Borate buffer pH 9, 0.01 M Solution of 0.01 M boric acid in water and 0.01 M potassium chloride in water adjusted to pH 9 using 1N sodium hydroxide. Buffer contained 0.0009% (w/v) sodium azide. - Details on test conditions:
- pH code Temperature I Temperature II Temperature III
pH 7 19.7°C ± 0.1°C 40.1°C ± 0.1°C 49.5°C ± 0.1°C
pH 9 23.2°C ± 4.4°C 40.1°C ± 0.1°C 49.5°C ± 0.1°C - Preliminary study:
- During method validation it was decided to prepare calibration curves in the buffers; however due to extreme hydrolysis it was not possible to create the calibration in buffers during the preliminary test. Therefore it was decided to proceed with the main study by preparation of the calibration solutions in water. Preliminary test is archived in raw data.
- Test performance:
- pH 4
Due to instantaneous hydrolysis it was technically not possible to perform the study at pH 4. Results are archived in raw data.
pH 7
The analytical results of the main study are given in Table 6 to Table 9 (see attached)
Small responses at the retention time of the test item were detected in the chromatograms in the blank buffer solutions. The concentrations were low and therefore considered not to have significant effect on the results of the hydrolysis.
The mean recoveries of the test item containing buffer solutions at t=0 fell within the criterion range of 70-110%. It demonstrated that the analytical method was adequate to support the hydrolysis study on the test item.
For testing of pseudo-first order kinetics the mean logarithms of the relative concentrations between 10% and 90% were plotted against time. At all temperatures linear relationships were obtained.
The rate constant (kobs) and half-life time of the test item at each temperature was obtained and the Arrhenius equation was used to determine the rate constant and half-life time at 25°C
pH 9
Small responses at the retention time of the test item were detected in the chromatograms in the blank buffer solutions. The concentrations were low and therefore considered not to have significant effect on the results of the hydrolysis.
(i.e. <70%). This had no impact on the hydrolysis parameters, since they are based on the difference in concentration. It demonstrated that the analytical method was adequate to support the hydrolysis study on the test item.
For testing of pseudo-first order kinetics the mean logarithms of the relative concentrations between 10% and 90% were plotted against time.
Nevertheless, when using those results hydrolysis appears to be faster at 40°C than at 50°C. This indicates that hydrolysis is not the only reaction that happens. - Transformation products:
- not specified
- pH:
- 7
- Temp.:
- 20 °C
- DT50:
- 1.5 h
- pH:
- 7
- Temp.:
- 25 °C
- DT50:
- 1.2 h
- pH:
- 7
- Temp.:
- 40 °C
- DT50:
- 0.61 h
- pH:
- 7
- Temp.:
- 50 °C
- DT50:
- 0.36 h
- pH:
- 9
- Temp.:
- 20 °C
- DT50:
- 91 h
- pH:
- 9
- Temp.:
- 25 °C
- DT50:
- 9 h
- pH:
- 9
- Temp.:
- 40 °C
- DT50:
- 0.007 h
- pH:
- 9
- Temp.:
- 50 °C
- DT50:
- 4.2 h
- Details on results:
- The preliminary test (Tier 1) and main study (Tier 2) were performed for the determination of the rate of hydrolysis of 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride at pH values normally found in the environment (pH 7-9). Half life at pH7 at 25 °C was 1.2 hours and 9hrs for pH 9.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The preliminary test (Tier 1) and main study (Tier 2) were performed for the determination of the rate of hydrolysis of 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride at pH values normally found in the environment (pH 7-9).
Reference
pH 7 |
pH 9 |
||
Temperature |
t½ |
Temperature |
t½ |
20 |
1.5 hours |
20 |
91 hours |
25 |
1.2 hours |
25 |
9.0 hours |
40 |
0.61 hours |
40 |
0.0074 hours |
50 |
0.36 hours |
50 |
4.2 hours |
Description of key information
The preliminary test (Tier 1) and main study (Tier 2) were performed for the determination of the rate of hydrolysis of 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride at pH values normally found in the environment (pH 7-9).
The half-life times of the test item were as follows:
pH 7 |
pH 9 |
||
Temperature |
t½ |
Temperature |
t½ |
20 |
1.5 hours |
20 |
91 hours |
25 |
1.2 hours |
25 |
9.0 hours |
40 |
0.61 hours |
40 |
0.0074 hours |
50 |
0.36 hours |
50 |
4.2 hours |
Key value for chemical safety assessment
- Half-life for hydrolysis:
- 1.5 h
- at the temperature of:
- 20 °C
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.