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Reaction mass of 3,3'-(1E,1'E)-(4,4'-(6-(bis(2-hydroxyethyl)amino)-1,3,5-triazine-2,4-diyl)bis(azanediyl)bis(3-methoxy-4,1-phenylene))bis(diazene-2,1-diyl)dibenzenesulfonic acid and disodium 3,3'-[[6-[bis(2-hydroxyethyl)amino]-1,3,5-triazine-2,4-diyl]bis[imino(3-methoxy-4,1-phenylene)azo]]bis[benzenesulphonate]
EC number: 943-704-1 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic plants other than algae
Administrative data
Link to relevant study record(s)
Description of key information
An OCED 221 study was carried out to determine the effects of the tested substance on the growth of the freshwater aquatic plant Lemna minor. After 7 days of exposure an EC50 value of > 100 mg/L (front number/biomass, growth rate), an EC10 value of 6.55 mg/L (front number, growth rate), an EC10 value of 1.01 mg/L (biomass, growth rate) and an a NOEC of 7.3 (frond number, growth rate) were determined.
Key value for chemical safety assessment
- EC50 for freshwater plants:
- 100 mg/L
- EC10 or NOEC for freshwater plants:
- 1.01 mg/L
Additional information
EC50 "> 100 mg/L"; EC50 and NOEC refer to biomass, growth rate
A growth inhibition test with the freshwater aquatic plant Lemna minor was conducted according to OECD 221 (March 2006) in order to investigate the aquatic phytotoxic effect of the tested substance. Lemna minor is a model organism for aquatic fresh water plants.
The aim of the study was to determine the effects of the tested substance on the growth of the freshwater aquatic plant Lemna minor. To achieve this, a series of test item concentrations in aqueous solutions was prepared (nominal 4.95, 10.9, 24.0, 52.7, 116 mg/L) and the plants were allowed to grow in these concentrations, as well as in controls without the test item, for a test period of 7 days under controlled conditions (temperature, illumination).
The data obtained were analysed using a regression model in order to estimate the concentration that would cause a x% growth inhibition, i.e. ECx (e.g. EC50, EC20 or EC10). As additional endpoints the No Observed Effect Concentration (NOEC) and Lowest Observed Effect Concentration (LOEC) will be reported if relevant.
Samples from the test solutions were analysed to determine actual levels of the test item at start of exposure period (time 0 days) and following 7 days of exposure. At time 0 days the measured concentrations range between 97–102 % and decrease after 7 days exposure at between 40–100 % of the nominal concentrations. Therefore, the test item concentration based on nominal concentration was not stable as it declined more than 20% throughout the exposure period.
All concentration levels were measured, and the biological results are additionally calculated and reported based on geometric mean measured concentrations at each concentration level.
A clear concentration-response relationship was observed for both biological parameters frond number and biomass evaluating growth rate and yield during the exposure period. According to the guideline OECD 221 and REACH Guidance R.7b (2017) front number is the primary measurement variable. For the measured variable 'front number growth rate' the endpoints EC50 >100 mg/L, EC10 6.55 mg/L and NOEC 7.3 mg/L were obtained based on geometric mean measured concentrations.
For the measured variable biomass growth rate the endpoints EC50 >100 mg /L, EC10 1.01 mg /L and NOEC < 2.73 mg /L were obtained based on geometric mean measured concentrations.
All validity criteria are met.This toxicity study is classified as acceptable and satisfies the guideline requirements for the growth inhibition test with Lemna minor.
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