Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 257-775-5 | CAS number: 52238-69-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- July from 17 to 20, 2006
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with generally accepted scientific standards and described in sufficient detail
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- 1984
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- GLP compliance:
- yes (incl. QA statement)
- Details on test solutions:
- Based on the results of a pre-experiment (without GLP), no stock solution could be prepared due to the limited water solubiliiy of the test item under the pre-test conditions. Therefore, 504.9 mg of the test item was directly weighed by means of an analytical balance and transferred to the test vessel with 284 ml- of tap water. The test item was mixed into the tap water by ultrasonic treatment for 15 minutes and intense stirring for three hours at room temperature in the dark, to dissolved a minimum amount of the test item and/or disperse it as homogeneously as possible. No emulsifers or solvents were used.
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Laboratory culture:
- Source: ARA Ergolz II, Füllinsdorf, Switzerland.
- Pretreatment:the sludge was washed three times with tap water by centrifugation and the supernatant liquid phase was decanted.
- Method of cultivation: an aliquot of washed sludge was suspended in tap water to obtain a concentration equivalent to 3 g dry material per liter. During the holding peiod of three days prior to use, the sludge was fed daily with 50 ml synthetic wastewater per liter and was kept at room temperature under continuous aeration until use.
- Initial biomass concentration: before use, the dry weight of the activated sludge was measured again in the inoculum used for the test and diluted to 4 g dry material per liter with tap water.
- PH: the pH of the activated sludge inoculum was measured to be 6.7. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Test temperature:
- 20 °C
- pH:
- Control: 7.3 - 8.1
Test vessel: 7.4 - 7.9 - Dissolved oxygen:
- Control: 8.2 - 7.7 mg O2/l
Test vessel: 8.3 - 8.5 mg O2/l - Nominal and measured concentrations:
- 1000 mg/l, nominal
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 2000-ml glass beakers.
- Aeration: all test media and controls were continuosly aerated by iintense stirring on magnetic stirrers to avoid possible foaming and/or stipping of the test item. The concentration of the dissolved oxygen did not drop below 2.5 mg/l during the incubation period and, just prior to measurement of the respiration rates, the dissolved oxygen were at least 8.5 mg/l.
- No. of vessels per concentration: 1 replicates.
- No. of vessels per control: 2 replicates, containing only tap water, synthetic wastewater and inoculum.
- No. of vessels per positive control: 1 replicate per concentration.
- Sludge amount: 200 ml.
- Sludge concentration: 4.0 g/l dry weight (corresponding to 1.6 g dry material per liter test medium). - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- IC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- At the limit test concentration of 1000 mg/l, test item was not completely dissolved in the test medium. Thus, the limit test concentration was above the water solubility limit of test item under the test conditions.
The test item had no significant inhibitory effect (ca -4.5 %;) on the respiration rate of activated sludge after the incubation period of 3 hours at the limit test concentration of 1000 mg/l.
Based on the physical appearance of the test suspension, the saturation concentration (water solubility limit of the substance under the test conditions) was lower than 1000 mg/l and resulted in ca -4.5 % inhibition after 3 hours contact time. Thus, the 3-hour NOEC is at the limit of water solubility of test item under the test conditions, or at least 1000 mg/l. The 3-hour EC20, EC50 and EC80 could not be calculated, but are clearly higher than the limit of water solubility of test item under the test conditions, or higher than 1000 mg/l.
The oxygen consumption rates of the two controls (run at the start and at the end of the test) differed only by 1 % (guideline-recommended maximum variation of 15 %). - Results with reference substance (positive control):
- The 3-hour EC50 of the reference item 3,5 dichlorophenol (positive control) was calculated to be 14 mg/l (the 95 % confidence limits were not calculable). The 3-hour EC50 is within the guideline-recommended range of 5 - 30 mg/l, thus confirming suitability of the activated sludge used.
- Conclusions:
- IC50 (3h) > 1000 mg/l (nominal)
NOEC (3h) ≥ 1000 mg/l (nominal) - Executive summary:
The inhibitory effect of the test item on the respiration rate of aerobic wastewater microorganisms of activated sludge was investigated in a 3-hour respiration inhibition test according to the EU Commission Directive 88/302/EEC, Part C.11 and the OECD Guideline for Testing of Chemicals, No 209.
A limit test with a nominal test concentration of 1000 mg/l was performed to demonstrate that the test item has no toxic effect on activated sludge microorganisms up to and including this concentration. In addition, two controls and three different concentrations of the reference item 3,5 -dichlorophenol (5, 16, and 50 mg/l) were tested in parallel. The results of these treatments confirmed suitability of the activated sludge and the method used.
The test item had no significant inhibitory effect (ca -4.5 %) on the respiration rate of activated sludge after the incubation period of three hours at the limit test concentration of 1000 mg/l. Based on the physical appearance of the test suspension, the saturation concentration (water solubility limit of test item under the test conditions) was lower than 1000 mg/l and resulted in less than contact time. Thus, the 3-hour NOEC of test item to activated sludge microorganisms is at the limit of water solubility under test conditions, or at least 1000 mg/l. The 3-hour EC20, EC50 and EC80 could not be calculated, but are clearly higher than the limit of water solubility of test teim.
Conclusion
IC50 (3h) > 1000 mg/l (nominal)
NOEC (3h) ≥ 1000 mg/l (nominal)
Reference
Oxygen consumption of activated sludge
Vessel | Oxygen consumption rate (mg O2/l/min) | Inhibition (%) | pH |
Oxygen concentration (mg O2/l) |
||
Start |
End |
Start |
End |
|||
Control |
1.483 | - | 7.4 | 8.0 | 8.2 | 8.7 |
Control | 1.463 | - | 7.3 | 8.1 | 8.2 | 8.7 |
Mean | 1.473 | |||||
Deviation (%) | 1.3 | |||||
Test item 1000 mg/l | 1.54 | -4.5 | 7.4 | 7.9 | 8.3 | 8.5 |
Positive control 5 mg/l | 1.355 | 8 | 7.3 | 8.1 | 8.4 | 8.7 |
Positive control 16 mg/l | 0.541 | 63.3 | 7.3 | 8.1 | 8.6 | 8.5 |
Positive control 50 mg/l | 0.125 | 91.5 | 7.2 | 8.0 | 8.9 | 8.6 |
Description of key information
No harmful/toxic to microorganisms (NOEC (3h) ≥ 1000 mg/l (nominal))
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 1 000 mg/L
Additional information
The inhibitory effect of the test item on the respiration rate of aerobic wastewater microorganisms of activated sludge was investigated in a 3-hour respiration inhibition test, according to the OECD Guideline 209. A limit test with a nominal test concentration of 1000 mg/l was performed.
The test item had no significant inhibitory effect (ca -4.5 %) on the respiration rate of activated sludge after the incubation period of three hours at the limit test concentration of 1000 mg/l. Based on the physical appearance of the test suspension, the saturation concentration (water solubility limit of test item under the test conditions) was lower than 1000 mg/l and resulted in less than contact time. Thus, the 3-hour NOEC of test item to activated sludge microorganisms is at the limit of water solubility under test conditions, or at least 1000 mg/l.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.