Aluminum, 2-(1,3-dihydro-1,3-dioxo-2H-inden-2-ylidene)-1,2-dihydro-6,7-quinolinedisulfonate complexes

Endpoint summary

Description of key information

Short term toxicity to aquatic invertebrates:

1.Test material when subjected to D. magna for 48 hrs the LC50 value was observed to be in the range 3.6-6.0 mg/l by chemical exposure.

2. The effect concentration of test material against Daphnia magna after 48 hrs was observed to be 4.271 mg/l

Thus based on the overall studies for the structurally and functionally similar read across chemical for Aluminum, 2-(1,3-dihydro-1,3-dioxo-2H-inden-2-ylidene)- 1,2-dihydro-6,7- quinolinedisulfonate complexes (94891-32-4), it can be concluded that the test chemical was toxic and classifies as aquatic chronic 2 as per the CLP classification criteria.

Toxicity to aquatic algae and cyanobacteria:

1. After 72 hours of exposure to test item to various nominal test concentrations, EC50 was determine to be 2.079 mg/l graphically and through probit analysis.

2. The effect concentration of test material on green algae on Raphidocelis subcapitata for growth rate after 72 h  was observed to be 1.20 mg/l

Based on the above study chemical was consider as toxic and classified as aquatic chronic 2 as per the CLP classification criteria.

Additional information

Summarized result for the determination of nature of chemical Aluminum, 2-(1,3-dihydro-1,3-dioxo-2H-inden-2-ylidene)-1,2-dihydro-6,7-quinolinedisulfonate complexes (94891 -32 -4) on the growth and other biological and physical activity of aquatic invertebrates, algae and cyanobacteria when chemical comes in contact with test organisms, by considering the data for structurally and functionally similar read across chemicals from various sources which are as follows:  

Short term toxicity to aquatic invertebrates:

Short term toxicity of Aluminum, 2-(1,3-dihydro-1,3-dioxo-2H-inden-2-ylidene)-1,2-dihydro-6,7-quinolinedisulfonate complexes (94891 -32 -4) on the growth and other biological activity of aquatic invertebrates is predicted on the basis of it structurally and functionally similar read across chemicals. The studies are as mentioned below:

 

Short term toxicity study was performed to determine the toxic effect of the structurally and functionally similar read across chemicals on the invertebrates from journal. Daphnia sp. Test material when used as a test material to evaluate its aquatic toxicity on Daphnia magna. The test animal was maintained. Twenty-four hours before the test, adult daphnids (gravid females) were sorted and the young one (neonates) produced from these adults were used in the experiment. Initially, a 24-h preliminary test was carried out at exposure concentrations of 100, 10, and 1.0 mg/LThe selected concentration for the test was 100, 80, 60, 40, 20, and 10 mg/L. After the exposure of test chemical for 24 hrs, the LC50 value was determine to be 6 mg/l and the 48hrs LC50 was determine in the range of 3.6 -6 mg/l. Based on the LC50, chemical was consider as toxic and classified as aquatic chronic 2 as per the CLP classification criteria.

 

Similar study was performed for another structurally and functionally similar read across chemicals. The acute aquatic toxicity was investigated with the 48 h daphnid immobilization assay for the test chemical. 24 h old neonatal Daphnia magna were used for the test. Test conducted under the static system for 48 hrs. The effect concentration was observed to be 4.271 mg/l after 48 h and the confidence interval C.I. was 3.680-4.957 mg/l. Based on the LC50, chemical was consider as toxic and can be consider to be classified as aquatic chronic 2 as per the CLP classification criteria.

 

Thus based on the overall studies from peer reviewed journal for the structurally and functionally similar read across chemical for Aluminum, 2-(1,3-dihydro-1,3-dioxo-2H-inden-2-ylidene)- 1,2-dihydro-6,7- quinolinedisulfonate complexes (94891-32-4), it can be concluded that the test chemical was toxic and classifies as aquatic chronic 2 as per the CLP classification criteria.

Toxicity to aquatic algae and cyanobacteria:

Short term toxicity of Aluminum, 2-(1,3-dihydro-1,3-dioxo-2H-inden-2-ylidene)-1,2-dihydro-6,7-quinolinedisulfonate complexes (94891 -32 -4) on the growth and other biological activity of aquatic algae is predicted on the basis of it structurally and functionally similar read across chemicals. The studies are as mentioned below:

 

Short term toxicity study was performed to determine the toxic effect of the structurally and functionally similar read across chemicals on the aquatic algae and cyanobacteria from study report. The effect of test item was studied on the growth of fresh water green alga Chlorella vulgaris. The study was conducted following OECD guideline 201- Alga, growth inhibition test. The test concentration chosen for the study were 6.25mg/l, 12.5mg/l, 25mg/l, 50mg/l, 100mg/l and 200mg/l. The test concentrations were prepared using stock solution of the test item using mineral media. The green alga was exposed to the test concentration for a period of 72 hours to observe average specific growth rate and % growth inhibition under the effect of the test item. EC50 calculated graphically through probit analysis was observed to be 2.079 mg/l. Based on the EC50, chemical was consider as toxic and classified as aquatic chronic 2 as per the CLP classification criteria.

 

Similar study was performed for another structurally and functionally similar read across chemicals from peer reviewed journal. The 72 h algal growth inhibition test with Raphidocelis subcapitata was done according to OECD 201 (2011) for the test chemical. Algae were routinely cultured at 25±1 °C with light in Cyanobacteria BG-11 Freshwater Solution. The culture was split twice a week, adding fresh medium. Algae were exposed to the treatment in sterile 24-well plates with 2 mL of treatment solution to the respective microplate well, for three days. The effect concentration of test material on green algae on Raphidocelis subcapitata for growth rate after 72 h was observed to be 1.20 mg/l.

 

Thus based on the overall studies for the structurally and functionally similar read across chemical for Aluminum, 2-(1,3-dihydro-1,3-dioxo-2H-inden-2-ylidene)- 1,2-dihydro-6,7- quinolinedisulfonate complexes (94891-32-4), it can be concluded that the test chemical was toxic and classifies as aquatic chronic 2 as per the CLP classification criteria.

Based on the overall studies chemical was consider as toxic and classified as aquatic chronic 2 as per the CLP classification criteria.