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EC number: 208-144-8 | CAS number: 512-56-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Repeated dose toxicity: oral
Administrative data
- Endpoint:
- repeated dose toxicity: oral, other
- Remarks:
- Males: 42 days including 14 days before mating. Females: from 14 days before mating to day 3 of lactation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 994
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 422 (Combined Repeated Dose Toxicity Study with the Reproduction / Developmental Toxicity Screening Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- Trimethyl phosphate
- EC Number:
- 208-144-8
- EC Name:
- Trimethyl phosphate
- Cas Number:
- 512-56-1
- Molecular formula:
- C3H9O4P
- IUPAC Name:
- trimethyl phosphate
- Test material form:
- liquid
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Crj: CD(SD)
- Sex:
- male/female
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- water
- Duration of treatment / exposure:
- Males: 42 days
Females: from 14 days prior to mating to Day 3 of lactation - Frequency of treatment:
- 7 days/week
Doses / concentrationsopen allclose all
- Dose / conc.:
- 0 mg/kg bw/day (nominal)
- Dose / conc.:
- 40 mg/kg bw/day (nominal)
- Dose / conc.:
- 100 mg/kg bw/day (nominal)
- Dose / conc.:
- 250 mg/kg bw/day (nominal)
- No. of animals per sex per dose:
- 13 animals/group
- Control animals:
- yes, concurrent vehicle
Results and discussion
Results of examinations
- Mortality:
- mortality observed, treatment-related
- Description (incidence):
- Twelve males and one female given 250 mg/kg died during the 4th to 6th week of the dosing period.
- Body weight and weight changes:
- effects observed, treatment-related
- Description (incidence and severity):
- Body weight gain of the males and females of the 250 mg/kg dose groups were significantly reduced.
Twelve and 2 pregnant females given 40 and 100 mg/kg, respectively, demonstrated significant decrease in body weight gain during mid and late pregnancy. - Food consumption and compound intake (if feeding study):
- effects observed, treatment-related
- Description (incidence and severity):
- Food consumption of the males of the 250 mg/kg dose group was significantly reduced.
- Haematological findings:
- effects observed, treatment-related
- Description (incidence and severity):
- In the males of the 100 mg/kg dose group, significantly decreased erythrocyte counts, hemoglobin concentration, hematocrit and A/G ratio, and increased platelet count, percentage of segmented neutrophils, cholinesterase activity, total cholesterol and calcium levels were noted. Similar alteration in hematological and clinical chemistry parameters was observed in one surviving male of the 250 mg/kg dose group.
- Organ weight findings including organ / body weight ratios:
- effects observed, treatment-related
- Description (incidence and severity):
- At terminal necropsy, compound-related alterations in organ weights included significant increases in the kidney weight of males of the 40 and 100 mg/kg dose groups and in the thymus weight of males of the 100 mg/kg dose group and of females of the 40 mg/kg dose group, and a significant decreases in the epididymal weight of males of 100 mg/kg dose group.
- Neuropathological findings:
- effects observed, treatment-related
- Description (incidence and severity):
- Progressive development of a paralytic gait and decreased motor activity in twelve males and one female.
- Histopathological findings: non-neoplastic:
- effects observed, treatment-related
- Description (incidence and severity):
- On histopathological examination, major lesions noted in males and females given 100 mg/kg or more included nephropathy characterized by tubular and papillary alteration such as increased eosinophilic droplets in tubular epithelium, increased regeneration of tubules and papillary necrosis, atrophy of the thymus, liver and testis, increased atretic follicles in the ovary (250 mg/kg female only), and degeneration of nerve fiber in the spinal cord or the peripheral nerves (e.g., sciatic nerve). The incidence and severity of these lesions increased with dose and were greater in males than females.
Effect levels
open allclose all
- Key result
- Dose descriptor:
- NOEL
- Effect level:
- < 40 mg/kg bw/day (nominal)
- Based on:
- test mat.
- Sex:
- male/female
- Basis for effect level:
- body weight and weight gain
- Key result
- Dose descriptor:
- LOEL
- Effect level:
- 40 mg/kg bw/day (nominal)
- Based on:
- test mat.
- Sex:
- male/female
- Basis for effect level:
- body weight and weight gain
Target system / organ toxicity
- Critical effects observed:
- no
Applicant's summary and conclusion
- Conclusions:
- The NOELs for repeat dose toxicity are considered to be less than 40 mg/kg/day for both sexes.
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