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EC number: 249-616-3 | CAS number: 29420-49-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 20 Jan - 20 Feb2003
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-B (Determination of the "Ready" Biodegradability - Modified OECD Screening Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 E (Ready biodegradability: Modified OECD Screening Test)
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Batch No.of test material: K100
- Sample no./year: 1069024/2002
- Purity test date: 10 Oct 2002
- Purity: 98.7%
- Expiration date of the lot/batch: August 12, 2004 - Oxygen conditions:
- aerobic
- Inoculum or test system:
- other: Secondary effluent of a treatment plant receiving predominantly domestic sewage
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Secondary effluent of a domestic sewage treatment plant (Wupper area water authority) treatment plant.
- Storage length: ca. 3 days based on collection date, test interval, and duration
- Pretreatment: separation of coarse particles by filtration - Duration of test (contact time):
- 28 d
- Initial conc.:
- 20 mg/L
- Based on:
- DOC
- Parameter followed for biodegradation estimation:
- DOC removal
- Parameter followed for biodegradation estimation:
- TOC removal
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Mineral medium (per test guideline)
- Additional substrate: No
- Solubilising agent: None
- Test temperature: 22 ± 2 °C
- Inoculum concentration: 0.5mL/L
- Continuous darkness: yes
SAMPLING
- Sampling frequency: Initial time, Days 7, 14, 21, 27 and 28.
CONTROL AND BLANK SYSTEM
- Inoculum blank: Duplicate
- Procedural control: Duplicate - Reference substance:
- benzoic acid, sodium salt
- Remarks:
- 20 mg DOC/L
- Key result
- Parameter:
- % degradation (DOC removal)
- Value:
- 14
- Sampling time:
- 28 d
- Validity criteria fulfilled:
- yes
- Remarks:
- difference of replicate values <20% (2%), reference substance at pass level by day 14 (day 7)
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- PFBS K-salt is not readily biodegradable (14% DOC removal, EU method C.4-B)
- Executive summary:
Ready biodegradability of PFBSK+ was assessed according to EU method C.4-B (modified OECD screening method, equivalent to OECD guideline 301E). Sodium benzoate was used as reference. PFBSK+ degraded 14% during the test period. PFBSK+ is not readily biodegradable.
The study was conducted in accord with internationally accepted guidelines under GLP criteria. Details are lacking about the conduct of the study, in particular regarding the inoculum, however the validity criteria were met. The study is considered reliable with restriction and is suitable for use in Risk Assessment, Classification & Labelling, and PBT Analysis.
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 2008
- Reliability:
- 3 (not reliable)
- Rationale for reliability incl. deficiencies:
- significant methodological deficiencies
- Remarks:
- Stated results contradicted by Figure
- Justification for type of information:
- Study is of the free acid form of PFBS. In buffered solution, biodegradation testing on the acid is equivalent to testing on the potassium salt.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Version / remarks:
- Automated test using OxiTop
- Deviations:
- not specified
- GLP compliance:
- no
- Specific details on test material used for the study:
- PFBS standard (98%) was obtained from Sigma–Aldrich (Germany)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: Aeration tank of a sewage treatment plant in Darmstadt, Germany
- Preparation of inoculum for exposure: aeration in a secondary effluent for 7 days at the test temperature. Coarse particles were removed by filtration - Duration of test (contact time):
- 40 d
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: As per OECD 301
- Additional substrate: No
- Solubilising agent: None
- Test temperature: 20 ± 0.2 °C
- Suspended solids concentration: 10 mL sludge suspension/L
TEST SYSTEM
- Culturing apparatus: 164-mL glass bottles containing a magnetic stir-bar and filled to top with mineral medium. Bottles were closed with rubber sleeve holding NaOH pellets to absorb evolved CO2, and capped with WTW OxiTop measuring devices to record pressure. Bottles were stirred in an incubation cabinet during the experiment.
- Number of culture flasks/concentration: Duplicate
SAMPLING
- Sampling frequency: Daily
- Sampling method: Automated data logging
CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes - Reference substance:
- acetic acid, sodium salt
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- < 1
- Sampling time:
- 28 d
- Remarks on result:
- other: PFBS was observed to biodegrade <1% of the total biodegradation within the experimental duration of 40 days.
- Parameter:
- % degradation (O2 consumption)
- Value:
- < 1
- Sampling time:
- 40 d
- Details on results:
- The test was conducted on several fluorochemical surfactants as well as PFBS. The figure shows roughly equivalent %ThOD for one surfactant (Zonyl) and PFBS. Nevertheless the report states a biodegradation of 13% for the surfactant and <1% for PFBS. Both values are contradicted by the depicted biodegradation curve, in which both materials have %ThOD values in the range 5-10%. In addition, the stated % ThOD for the reference substance, 70.4%, is not supported by the biodegradation curve. It is possible that the biodegradation curve shows results for a single run whereas numeric results are means of several runs. The conclusion that PFBS shows little biodegradation is not questioned, but the value is not known with certainty.
- Validity criteria fulfilled:
- not specified
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- PFBS was biodegraded <1% in an automated test conducted according to OECD 301F. Results for the free acid are directly applicable to PFBSK+.
- Executive summary:
PFBS biodegradation was examined in an automated test conducted according to OECD 301F. Several fluorinated surfactants were examined in the same study. Sodium acetate was used as reference substance, and the test duration was extended to 40 days. PFBS was biodegraded <1% over the test interval. PFBS is not readily biodegradable. In buffered media, biodegradation testing on the free acid is equivalent to testing on the salt. Therefore, results for PFBS are directly applicable to PFBSK+.
The test was conducted according to internationally accepted guideline using an automated test system. However, limited details are available on the study method, and the depicted biodegradation-time curve contradicts numeric results. The central conclusion of lack of biodegradation is not disputed, but the numeric result is not considered reliable.
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 2008
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
- Justification for type of information:
- Study is of the free acid form of PFBS. In buffered solution, biodegradation testing on the acid is equivalent to testing on the potassium salt.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Deviations:
- not specified
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- PFBS standard (98%) was obtained from Sigma–Aldrich (Germany)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- natural water: freshwater
- Remarks:
- Rhine River water
- Details on inoculum:
- - Source of inoculum: taken at Rhine km 463.6 (Biebesheim, Germany) in spring 2008
- Duration of test (contact time):
- 28 d
- Initial conc.:
- 73 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: As per OECD 301
- Additional substrate: No
- Solubilising agent: None
- Test temperature: 20 ± 1 °C
- Continuous darkness: yes
- Number of culture flasks/concentration: Duplicate
- Inoculum blank: Yes
- Sampling frequency: Weekly - Reference substance:
- acetic acid, sodium salt
- Preliminary study:
- Total organic carbon (TOC) was measured using EPA Method 415.1. The value was 0.15 mg C/mg sample. Calculated ThOD was 0.45 mg O2/mg sample
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- < 3
- Sampling time:
- 28 d
- Details on results:
- The test was conducted on several fluorochemical surfactants as well as PFBS. The degree of biodegradation was deemed not significant by study authors, with a value <3% reported. No statistics were provided or described.
- Results with reference substance:
- While the paper indicates that sodium acetate was used as a reference substance, no results are provided. One of the fluorinated substances attained 80% degradation within 28-days, indicating a viable inoculum.
- Validity criteria fulfilled:
- not specified
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- PFBS was not biodegraded in a closed bottle test (OECD 301D) conducted with a Rhine River inoculum. Results for the free acid are directly applicable to PFBSK+.
- Executive summary:
PFBS biodegradation was examined in an closed bottle test conducted according to OECD 301D. Several fluorinated surfactants were examined in the same study. Sodium acetate was used as reference substance. PFBS was biodegraded <3% over the test interval. PFBS is not readily biodegradable. In buffered media, biodegradation testing on the free acid is equivalent to testing on the salt. Therefore, results for PFBS are directly applicable to PFBSK+.
The test was conducted according to an internationally accepted guideline. However, limited details are available on the study method, and the study was not done under GLP criteria. Therefore, the study is deemed reliable with restrictions and is suitable as supporting information for Risk Assessment, Classification & Labeling, and PBT Analysis.
Referenceopen allclose all
Table 1, DOC measurements during the biodegradation test
Condition |
Replicate |
Measurement |
DOC (mg/L), Day 0 |
Day 7 |
Day 14 |
Day 21 |
Day 27 |
Day 28 |
Test item plus inoculum |
A |
1 |
22.0 |
22.0 |
20.0 |
20.0 |
19.0 |
19.0 |
2 |
23.0 |
22.0 |
21.0 |
21.0 |
20.0 |
20.0 |
||
mean |
22.5 |
22.0 |
20.5 |
20.5 |
19.5 |
19.5 |
||
B |
1 |
22.0 |
22.0 |
30.0 |
19.0 |
19.0 |
19.0 |
|
2 |
22.0 |
23.0 |
21.0 |
20.0 |
20.0 |
20.0 |
||
mean |
22.0 |
22.5 |
25.5 |
19.5 |
19.5 |
19.5 |
||
Blank inoculum |
A |
1 |
3.0 |
2.0 |
1.0 |
2.0 |
2.0 |
3.0 |
2 |
3.0 |
2.0 |
1.0 |
2.0 |
2.0 |
3.0 |
||
mean |
3.0 |
2.0 |
1.0 |
2.0 |
2.0 |
3.0 |
||
B |
1 |
3.0 |
2.0 |
1.0 |
2.0 |
2.0 |
3.0 |
|
2 |
3.0 |
2.0 |
1.0 |
2.0 |
2.0 |
3.0 |
||
mean |
3.0 |
2.0 |
1.0 |
2.0 |
2.0 |
3.0 |
||
mean of blanks |
|
3.0 |
2.0 |
1.0 |
2.0 |
2.0 |
3.0 |
|
Reference substance plus inoculum |
A |
1 |
23.0 |
3.0 |
3.0 |
3.0 |
3.0 |
3.0 |
2 |
23.0 |
3.0 |
3.0 |
3.0 |
3.0 |
3.0 |
||
mean |
23.0 |
3.0 |
3.0 |
3.0 |
3.0 |
3.0 |
||
B |
1 |
23.0 |
3.0 |
3.0 |
3.0 |
3.0 |
2.0 |
|
2 |
24.0 |
3.0 |
3.0 |
3.0 |
3.0 |
3.0 |
||
mean |
23.5 |
3.0 |
3.0 |
3.0 |
3.0 |
2.5 |
Table 2, percent biodegradation
Condition |
Replicate |
% biodeg, Day 0 |
Day 7 |
Day 14 |
Day 21 |
Day 27 |
Day 28 |
Test item plus inoculum |
A |
0 |
0 |
0 |
5 |
10 |
15 |
B |
0 |
0 |
0 |
8 |
8 |
13 |
|
mean |
0 |
0 |
0 |
7 |
9 |
14 |
|
Reference substance plus inoculum |
A |
0 |
95 |
90 |
95 |
95 |
100 |
B |
0 |
95 |
90 |
95 |
95 |
100 |
|
mean |
0 |
95 |
90 |
95 |
95 |
100 |
Description of key information
PFBSK+ is not readily biodegradable.
Key value for chemical safety assessment
Additional information
Ready biodegradability of PFBSK+ was examined under three test guidelines. The key study was done under EU method C.4-B (modified OECD screening method, equivalent to OECD guideline 301E) and showed 14% biodegradation during the test period. The study was GLP-compliant and is considered reliable with restrictions. The other two studies were done on the free acid (PFBS) rather than the potassium salt. However, because the test materials were tested in standard mineral medium buffered to pH ca. 7.4, testing on the free acid is equivalent to testing on the potassium salt. Both supporting studies were published in a scientific journal are not GLP-compliant and are considered reliable with restrictions. PFBS was not biodegraded (<3%) in a closed bottle test (OECD 301D) conducted using a Rhine River inoculum. Also, PFBS was not biodegraded in an automated test conducted according to OECD 301F, although the depicted biodegradation-time curve contradicts the reported numeric results. The central conclusion of lack of biodegradation is not disputed for the 301F study, but its numeric result is not considered reliable.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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